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Characterization of infectious bronc...

University of Delaware., Department of Animal and Food Sciences.

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Characterization of infectious bronchitis virus isolates discovered during the 2004 avian influenza outbreak of the Delmarva Peninsula.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of infectious bronchitis virus isolates discovered during the 2004 avian influenza outbreak of the Delmarva Peninsula./
Author:	Foltz, Jeffrey Andrew.
Description:	128 p.
Notes:	Adviser: Jack Gelb.
Contained By:	Masters Abstracts International47-02.
Subject:	Agriculture, Animal Pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1457117
ISBN:	9780549753568

Characterization of infectious bronchitis virus isolates discovered during the 2004 avian influenza outbreak of the Delmarva Peninsula.
Foltz, Jeffrey Andrew.

Characterization of infectious bronchitis virus isolates discovered during the 2004 avian influenza outbreak of the Delmarva Peninsula. - 128 p.

Adviser: Jack Gelb.

Thesis (M.S.)--University of Delaware, 2008.

Numerous field isolates of avian infectious bronchitis virus (IBV) were recovered from flocks during a limited avian influenza outbreak on the Delmarva Peninsula during the winter of 2004. A number of these isolates came from flocks with elevated mortality rates. Ten isolates recovered from farms with mortality of 4 per 1000 per day to 7.5 per 1000 per day were characterized.

ISBN: 9780549753568Subjects--Topical Terms:

1021764
Agriculture, Animal Pathology.

Characterization of infectious bronchitis virus isolates discovered during the 2004 avian influenza outbreak of the Delmarva Peninsula.
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020 $a 9780549753568
035 $a (UMI)AAI1457117
035 $a AAI1457117
040 $a UMI $c UMI
100 1 $a Foltz, Jeffrey Andrew. $3 1031184
245 1 0 $a Characterization of infectious bronchitis virus isolates discovered during the 2004 avian influenza outbreak of the Delmarva Peninsula.
300 $a 128 p.
500 $a Adviser: Jack Gelb.
500 $a Source: Masters Abstracts International, Volume: 47-02, page: .
502 $a Thesis (M.S.)--University of Delaware, 2008.
520 $a Numerous field isolates of avian infectious bronchitis virus (IBV) were recovered from flocks during a limited avian influenza outbreak on the Delmarva Peninsula during the winter of 2004. A number of these isolates came from flocks with elevated mortality rates. Ten isolates recovered from farms with mortality of 4 per 1000 per day to 7.5 per 1000 per day were characterized.
520 $a Tracheal swab samples from flocks being tested for the avian influenza virus were sent to the Allen Laboratory of the University of Delaware for virus isolation attempts performed in specific pathogen free (SPF) embryonated eggs. Many of the inoculated embryos showed stunting and curling; lesions indicative of possible infection with IBV. The presence of IBV in suspected isolates was verified by reverse transcription-polymerase chain reaction (RT-PCR).
520 $a Identification of the IBV genotype was determined. A fragment of the S1 subunit of the spike glycoprotein of isolates positive for IBV was amplified and sequenced. An alignment was performed between these S1 gene sequences and sequences of reference strains of IBV (Arkansas, Connecticut, Massachusetts, and Delaware 072) and commercial vaccines for IBV used along the peninsula.
520 $a A total of 101 isolates were successfully sequenced. Eighty two of the isolates were of the Arkansas genotype (92.6% to 100% amino acid similarity). Four of the isolates were found to be the Connecticut genotype (97.1% to 98.9% amino acid similarity). Fourteen of the isolates had an amino acid similarity closest to the Massachusetts genotype (94.6% to 100% amino acid similarity). The remaining IBV isolate was identified as Delaware 072 (90% amino acid similarity).
520 $a Additional characterization was conducted on a group of ten isolates. All of the isolates came from flocks experiencing mortality of at least 4 birds per 1000 per day. Non-vaccinated broiler chicks were inoculated with the ten isolates under study in order to determine pathogenicity. A vaccination-challenge of immunity study of specific pathogen free (SPF) chickens was performed to determine the effectiveness of vaccination against challenge of isolates of the same genotype. The presence of chicken anemia virus (CAV) in isolates was determined by a nested PCR.
590 $a School code: 0060.
650 4 $a Agriculture, Animal Pathology. $3 1021764
650 4 $a Biology, Virology. $3 1019068
690 $a 0476
690 $a 0720
710 2 $a University of Delaware. $b Department of Animal and Food Sciences. $3 1029904
773 0 $t Masters Abstracts International $g 47-02.
790 $a 0060
790 1 0 $a Emara, Marlene $e committee member
790 1 0 $a Gelb, Jack $e committee member
790 1 0 $a Gelb, Jack, $e advisor
790 1 0 $a Schmidt, Carl $e committee member
791 $a M.S.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1457117