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Establishment of transformation syst...

Iowa State University., Agronomy.

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Establishment of transformation systems using mature seeds of maize inbred lines.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Establishment of transformation systems using mature seeds of maize inbred lines./
Author:	Martinez Nicolas, Juan Carlos.
Description:	97 p.
Notes:	Adviser: Kan Wang.
Contained By:	Masters Abstracts International46-04.
Subject:	Agriculture, Agronomy. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1450155
ISBN:	9780549423324

Establishment of transformation systems using mature seeds of maize inbred lines.
Martinez Nicolas, Juan Carlos.

Establishment of transformation systems using mature seeds of maize inbred lines. - 97 p.

Adviser: Kan Wang.

Thesis (M.S.)--Iowa State University, 2008.

This research program aimed at developing a transformation system using maize mature seeds as starting material for inbred lines B73, B104, H99, Mo17 and W22.

ISBN: 9780549423324Subjects--Topical Terms:

1018679
Agriculture, Agronomy.

Establishment of transformation systems using mature seeds of maize inbred lines.
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020 $a 9780549423324
035 $a (UMI)AAI1450155
035 $a AAI1450155
040 $a UMI $c UMI
100 1 $a Martinez Nicolas, Juan Carlos. $3 1031177
245 1 0 $a Establishment of transformation systems using mature seeds of maize inbred lines.
300 $a 97 p.
500 $a Adviser: Kan Wang.
500 $a Source: Masters Abstracts International, Volume: 46-04, page: 1936.
502 $a Thesis (M.S.)--Iowa State University, 2008.
520 $a This research program aimed at developing a transformation system using maize mature seeds as starting material for inbred lines B73, B104, H99, Mo17 and W22.
520 $a Maize is one of the most important crops for plant biologists not only for its tremendous values for agriculture but also for its wealthy genetic information for biology. Recent years, the advent of biotechnology has further expanded the possibility of this crop. One of the most important tools for crop improvement and basic biological studies is genetic transformation. While plant genetic transformation using either Agrobacterium tumefaciens or biolistic gun as delivery systems has been available for more than 20 years, genetic transformation of many plant species, especially cereal crops, is still a challenging task for most laboratories.
520 $a The efficiency in genetic transformation depends upon the establishment of a robust and reproducible plant tissue culture system as well as an efficient delivery system for transformation. For current maize transformation system, immature embryos are routinely used as starting material for Agrobacterium infection or particle bombardment. The major problem for using the immature embryos is that it requires maize plants to be grown in the greenhouse year-round to meet the research demands. This practice requires large greenhouse spaces, quality growth conditions and experienced supporting staff.
520 $a One other problem with the current maize transformation system is that only Hi II, a hybrid germplasm bred specifically for tissue culture purposes, is amendable for transformation. For the maize community, inbred lines are the most desirable target for transformation due to their genetic background and agronomic importance in crop improvement.
520 $a The first challenge in this study was to obtain sterilized mature embryo material for callus initiation. We have established an efficient and optimized seed sterilization protocol for maize seeds harvested from field-grown plants which have an increased amount of seed-borne pathogens. Our protocol ensures 98-100% sterility of plant material without noticeable compromise of the vigor and callus initiation.
520 $a Two different approaches: shoot meristematic cultures (SMC) and somatic embryogenic cultures (SEC) have been evaluated to obtain transformation-target tissue. Although SMC of different inbred lines could be obtained in a non-genotype dependent fashion, and GUS expression was detected when performing genetic transformation using Agrobacterium-mediated and the biolistic gun, no plants were recovered using this tissue culture system. On the other hand SEC was observed in all inbred lines at different levels, however just H99 responded with high frequency on callus induction medium (71%). Bombardment of SEC of inbred line H99 resulted in fertile transgenic plants. Analysis of progeny indicated that both gus and bar transgenes have been transmitted to the next generation. Bialaphos resistant callus frequency (BRCF) ranged from 24.1% to 74% with this system.
590 $a School code: 0097.
650 4 $a Agriculture, Agronomy. $3 1018679
650 4 $a Agriculture, Plant Culture. $3 1018669
690 $a 0285
690 $a 0479
710 2 $a Iowa State University. $b Agronomy. $3 1023712
773 0 $t Masters Abstracts International $g 46-04.
790 $a 0097
790 1 0 $a Becraft, Philip W. $e committee member
790 1 0 $a Lee, Michael $e committee member
790 1 0 $a Wang, Kan, $e advisor
791 $a M.S.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1450155