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Discovering new regulon members of t...

University of California, Davis.

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Discovering new regulon members of the Escherichia coli EnvZ/OmpR two-component signal transduction system.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Discovering new regulon members of the Escherichia coli EnvZ/OmpR two-component signal transduction system./
Author:	Goh, Ee-Been.
Description:	408 p.
Notes:	Source: Dissertation Abstracts International, Volume: 69-06, Section: B, page: 3395.
Contained By:	Dissertation Abstracts International69-06B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3317927
ISBN:	9780549670322

Discovering new regulon members of the Escherichia coli EnvZ/OmpR two-component signal transduction system.
Goh, Ee-Been.

Discovering new regulon members of the Escherichia coli EnvZ/OmpR two-component signal transduction system. - 408 p.

Source: Dissertation Abstracts International, Volume: 69-06, Section: B, page: 3395.

Thesis (Ph.D.)--University of California, Davis, 2008.

Bacterial two-component signal transduction systems play a crucial role in regulating cellular responses to environmental changes. One of the most studied two-component system is the Escherichia coli K-12 EnvZ/OmpR system. EnvZ, is a sensor kinase that detects and relay environmental signals to its cognate response regulator, OmpR via phosphorylation. Phosphorylation of OmpR activates the protein and facilitates the binding of OmpR to DNA sequences upstream of its target genes. Two of the best characterized OmpR targets are the genes encoding the major outer membrane porins, OmpF and OmpC. Most of what is known about the EnvZ/OmpR signal transduction pathway comes from our understanding in the regulation of ompF and ompC. Since transcription of ompF and ompC are impacted by osmolarity changes, it was established that EnvZ/OmpR plays a key role in regulating the osmolarity stress response.

ISBN: 9780549670322Subjects--Topical Terms:

1017734
Biology, Microbiology.

Discovering new regulon members of the Escherichia coli EnvZ/OmpR two-component signal transduction system.
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100 1 $a Goh, Ee-Been. $3 1030056
245 1 0 $a Discovering new regulon members of the Escherichia coli EnvZ/OmpR two-component signal transduction system.
300 $a 408 p.
500 $a Source: Dissertation Abstracts International, Volume: 69-06, Section: B, page: 3395.
502 $a Thesis (Ph.D.)--University of California, Davis, 2008.
520 $a Bacterial two-component signal transduction systems play a crucial role in regulating cellular responses to environmental changes. One of the most studied two-component system is the Escherichia coli K-12 EnvZ/OmpR system. EnvZ, is a sensor kinase that detects and relay environmental signals to its cognate response regulator, OmpR via phosphorylation. Phosphorylation of OmpR activates the protein and facilitates the binding of OmpR to DNA sequences upstream of its target genes. Two of the best characterized OmpR targets are the genes encoding the major outer membrane porins, OmpF and OmpC. Most of what is known about the EnvZ/OmpR signal transduction pathway comes from our understanding in the regulation of ompF and ompC. Since transcription of ompF and ompC are impacted by osmolarity changes, it was established that EnvZ/OmpR plays a key role in regulating the osmolarity stress response.
520 $a However, most of the other OmpR target genes are not regulated in a similar fashion as ompF and ompC and respond to other environmental factors. Therefore, aside from regulating the osmolarity stress response, EnvZ/OmpR may also have a global role in the general cellular stress response. Unfortunately, little is known about how OmpR is involved in the regulation of these other genes, which has made it hard to uncover other possible role that EnvZ/OmpR may have in the cell. Therefore, to better understand EnvZ/OmpR's global role in the E. coli stress response, further identification and characterization of new OmpR regulated genes are needed.
520 $a In this study, we developed a strategy that combines genomic and computational approaches to identify potential OmpR regulated genes. This strategy successfully identified several new OmpR regulon members including tppB, which encodes the tripeptide permease and dnaK, which encodes a heat shock chaperone. Detailed characterization was conducted on tppB and dnaK and has allowed us to gain insight into the global role of EnvZ/OmpR system in the cellular adaptive and stress response. Finally, we used the same strategy to successfully identified new regulon members of the NarX/NarL two-component system and prove that this approach is valuable for studying other two-component systems.
590 $a School code: 0029.
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0410
710 2 0 $a University of California, Davis. $3 1018682
773 0 $t Dissertation Abstracts International $g 69-06B.
790 $a 0029
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3317927