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Efficiency of LHRH immunization as a...

Washington State University.

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Efficiency of LHRH immunization as a male sterilization vaccine.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Efficiency of LHRH immunization as a male sterilization vaccine./
Author:	Hernandez, Jennifer A.
Description:	118 p.
Notes:	Chair: Jerry J. Reeves.
Contained By:	Dissertation Abstracts International66-01B.
Subject:	Agriculture, Animal Culture and Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3162110
ISBN:	9780496958566

Efficiency of LHRH immunization as a male sterilization vaccine.
Hernandez, Jennifer A.

Efficiency of LHRH immunization as a male sterilization vaccine. - 118 p.

Chair: Jerry J. Reeves.

Thesis (Ph.D.)--Washington State University, 2004.

The objective of the studies presented herein is to examine the effectiveness of immunocastration using recombinant LHRH fusion proteins compared to surgical castration. In experiment 1, reproductive and hormonal effects of recombinant fusion proteins were investigated in Nelore bulls in Brazil. This experiment was divided into two studies. In study I, animals were separated into three treatment groups: intact; surgically castrated; and LHRH immunocastrated. LHRH immunocastrated bulls were immunized against a cocktail of two recombinant LHRH-7 fusion proteins. In study II, a fourth treatment group was implement in which half of the LHRH immunocastrated bulls received an additional immunization, and this study extended and additional year. LHRH antibodies were not detectable in the castrate or intact animals in either study. In both studies, scrotal circumference decreased as LHRH antibody binding increased in immunized bulls. By d 287 serum testosterone concentrations for all castrate groups in both studies was decreased when compared to intact controls. In experiment 2, carcass characteristics were examined for the bulls in study I. Average tenderness scores were inferior for intact bulls compared to immunized and castrated animals. Additionally, both castrated and immunized animals had greater marbling and percent carcass fat than the intact bulls. The producer received premium steer price for LHRH immunized bulls over intact controls at the time of slaughter. These data support the concept that immunocastration of bulls at 2 yrs of age is successful in inducing a castrate like effect and has practical application as a tool for managing grass fattened bulls in Brazil. Experiment 3 was conducted to gain a deeper understanding of immunocastration on a molecular and cellular level. Mature Sprague Dawley male rats were divided into four treatment groups: intact, surgically castrated, LHRH immunocastrated, and rats which received both surgical and LHRH immunological castration. Interest in the cellular aspects of LHRH active immunization arises from the knowledge that although immunocastration and surgical castration are both effective methods used to suppress reproduction, the manner by which each is achieved is unique. Microarray analysis was utilized to examine gene expression changes in the two types of castration.

ISBN: 9780496958566Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

Efficiency of LHRH immunization as a male sterilization vaccine.
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100 1 $a Hernandez, Jennifer A. $3 1029861
245 1 0 $a Efficiency of LHRH immunization as a male sterilization vaccine.
300 $a 118 p.
500 $a Chair: Jerry J. Reeves.
500 $a Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0031.
502 $a Thesis (Ph.D.)--Washington State University, 2004.
520 $a The objective of the studies presented herein is to examine the effectiveness of immunocastration using recombinant LHRH fusion proteins compared to surgical castration. In experiment 1, reproductive and hormonal effects of recombinant fusion proteins were investigated in Nelore bulls in Brazil. This experiment was divided into two studies. In study I, animals were separated into three treatment groups: intact; surgically castrated; and LHRH immunocastrated. LHRH immunocastrated bulls were immunized against a cocktail of two recombinant LHRH-7 fusion proteins. In study II, a fourth treatment group was implement in which half of the LHRH immunocastrated bulls received an additional immunization, and this study extended and additional year. LHRH antibodies were not detectable in the castrate or intact animals in either study. In both studies, scrotal circumference decreased as LHRH antibody binding increased in immunized bulls. By d 287 serum testosterone concentrations for all castrate groups in both studies was decreased when compared to intact controls. In experiment 2, carcass characteristics were examined for the bulls in study I. Average tenderness scores were inferior for intact bulls compared to immunized and castrated animals. Additionally, both castrated and immunized animals had greater marbling and percent carcass fat than the intact bulls. The producer received premium steer price for LHRH immunized bulls over intact controls at the time of slaughter. These data support the concept that immunocastration of bulls at 2 yrs of age is successful in inducing a castrate like effect and has practical application as a tool for managing grass fattened bulls in Brazil. Experiment 3 was conducted to gain a deeper understanding of immunocastration on a molecular and cellular level. Mature Sprague Dawley male rats were divided into four treatment groups: intact, surgically castrated, LHRH immunocastrated, and rats which received both surgical and LHRH immunological castration. Interest in the cellular aspects of LHRH active immunization arises from the knowledge that although immunocastration and surgical castration are both effective methods used to suppress reproduction, the manner by which each is achieved is unique. Microarray analysis was utilized to examine gene expression changes in the two types of castration.
590 $a School code: 0251.
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