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A comparison of the role of protein ...

State University of New York at Buffalo., Biological Sciences.

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A comparison of the role of protein kinase C in enamel matrix derivative and transforming growth factor-beta stimulated human gingival fibroblasts .

Record Type:	Language materials, printed : Monograph/item
Title/Author:	A comparison of the role of protein kinase C in enamel matrix derivative and transforming growth factor-beta stimulated human gingival fibroblasts ./
Author:	Tawati, Afaf.
Description:	45 p.
Notes:	Adviser: Rosemary Dziak.
Contained By:	Masters Abstracts International47-01.
Subject:	Health Sciences, Dentistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=1458464
ISBN:	9780549773030

A comparison of the role of protein kinase C in enamel matrix derivative and transforming growth factor-beta stimulated human gingival fibroblasts .
Tawati, Afaf.

A comparison of the role of protein kinase C in enamel matrix derivative and transforming growth factor-beta stimulated human gingival fibroblasts . - 45 p.

Adviser: Rosemary Dziak.

Thesis (M.S.)--State University of New York at Buffalo, 2008.

Aim. EMD has been advocated as a periodontal regenerative material, but its mechanism of action remains unclear. Recent studies provide evidence of the presence of several growth factors namely, TGF-beta & BMPs, which are thought to play a role in the mechanism of action of EMDs. Hence, the aims of this study are to compare the effect of enamel matrix derivative and transforming growth factor - beta on human gingival fibroblasts proliferation and differentiation. In addition, the role of a PKC dependant pathway in these cells differentiation and proliferation will be investigated. Finally, the dose response effect of TGF-beta on HGF will be evaluated.

ISBN: 9780549773030Subjects--Topical Terms:

1019378
Health Sciences, Dentistry.

A comparison of the role of protein kinase C in enamel matrix derivative and transforming growth factor-beta stimulated human gingival fibroblasts .
LDR:03423nam 2200301 a 45 001 858322
005 20100712
008 100712s2008 ||||||||||||||||| ||eng d
020 $a 9780549773030
035 $a (UMI)AAI1458464
035 $a AAI1458464
040 $a UMI $c UMI
100 1 $a Tawati, Afaf. $3 1025374
245 1 2 $a A comparison of the role of protein kinase C in enamel matrix derivative and transforming growth factor-beta stimulated human gingival fibroblasts .
300 $a 45 p.
500 $a Adviser: Rosemary Dziak.
500 $a Source: Masters Abstracts International, Volume: 47-01, page: 0317.
502 $a Thesis (M.S.)--State University of New York at Buffalo, 2008.
520 $a Aim. EMD has been advocated as a periodontal regenerative material, but its mechanism of action remains unclear. Recent studies provide evidence of the presence of several growth factors namely, TGF-beta & BMPs, which are thought to play a role in the mechanism of action of EMDs. Hence, the aims of this study are to compare the effect of enamel matrix derivative and transforming growth factor - beta on human gingival fibroblasts proliferation and differentiation. In addition, the role of a PKC dependant pathway in these cells differentiation and proliferation will be investigated. Finally, the dose response effect of TGF-beta on HGF will be evaluated.
520 $a Methods. HGFs were cultured in alpha-minimum essential medium (alpha-MEM) with 10% fetal calf serum) and allowed to attach for 24 hours. Then, EMD 25microg/ml, 50microg/ml & TGF-beta 10ng/ml were added and general PKC inhibitor 40 nM & compared to the negative control in terms of cell proliferation via a tritiated thymidine assay at 24 hours. The differentiation of cells within the treatment groups was also investigated by an alkaline phosphatase assay at 72 & 96 hours. An alkaline phosphatase assay was also run at 72 hours for the different TGF-beta concentrations of 0.1, 1, 10 & 100ng/ml.
520 $a Results. TGF-beta & EMD showed a significant uptake of tritiated thhymidine by treated HGF; meaning increased mitogenic effect when compared to control (p<0.5). The results of the alkaline phosphatase assay show no significant effect of EMD 50microg/ml when compared to the control, an inconsistent effect was observed for EMD 25 microg/ml, and a significant decrease with TGF-beta 10ng/ml. PKC inhibitor decreased the enzyme activity for both EmdogainRTM concentrations significantly. Finally, the different concentrations of TGF-beta resulted in an insignificant increase in the enzyme activity.
520 $a Conclusion. Within the limits of this study, it is clear that the enhanced soft tissue healing observed clinically after application of EmdogainRTM is the result of increased cellular proliferation rather than differentiation. A PKC signaling pathway might be involved in the action of HGF cells stimulated by EmdogainRTM, although the proliferation of these cells is not dependant on this pathway. The results do not exclude the possibility that TGF-beta is an active component of EMD. Nevertheless, this effect seems to be partial and governed by the presence of other growth factors and EMPs.
590 $a School code: 0656.
650 4 $a Health Sciences, Dentistry. $3 1019378
690 $a 0567
710 2 $a State University of New York at Buffalo. $b Biological Sciences. $3 1025373
773 0 $t Masters Abstracts International $g 47-01.
790 $a 0656
790 1 0 $a Dziak, Rosemary, $e advisor
791 $a M.S.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=1458464