東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Advances in forensic science: Improv...

University of Virginia.

Linked to FindBook

Google Book

Amazon

博客來

Advances in forensic science: Improving sexual assault evidence analysis.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Advances in forensic science: Improving sexual assault evidence analysis./
Author:	Norris, Jessica Voorhees.
Description:	184 p.
Notes:	Adviser: James P. Landers.
Contained By:	Dissertation Abstracts International70-02B.
Subject:	Anthropology, Medical and Forensic. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3348723
ISBN:	9781109043822

Advances in forensic science: Improving sexual assault evidence analysis.
Norris, Jessica Voorhees.

Advances in forensic science: Improving sexual assault evidence analysis. - 184 p.

Adviser: James P. Landers.

Thesis (Ph.D.)--University of Virginia, 2008.

Genetic analysis of DNA obtained from vaginal swabs is well-established for investigation of sexual assault cases. Although conventional analysis techniques are effective, they are time-consuming and laborious, which has produced a substantial backlog of casework samples to be analyzed. Microfluidic technologies present one solution to this problem. The work presented describes techniques aimed at improving forensic genetic analysis techniques, and specifically addresses procedures necessary for sexual assault evidence analysis, where male and female DNA must be separately interrogated.

ISBN: 9781109043822Subjects--Topical Terms:

1020279
Anthropology, Medical and Forensic.

Advances in forensic science: Improving sexual assault evidence analysis.
LDR:03328nam 2200301 a 45 001 858301
005 20100712
008 100712s2008 ||||||||||||||||| ||eng d
020 $a 9781109043822
035 $a (UMI)AAI3348723
035 $a AAI3348723
040 $a UMI $c UMI
100 1 $a Norris, Jessica Voorhees. $3 1025350
245 1 0 $a Advances in forensic science: Improving sexual assault evidence analysis.
300 $a 184 p.
500 $a Adviser: James P. Landers.
500 $a Source: Dissertation Abstracts International, Volume: 70-02, Section: B, page: 0990.
502 $a Thesis (Ph.D.)--University of Virginia, 2008.
520 $a Genetic analysis of DNA obtained from vaginal swabs is well-established for investigation of sexual assault cases. Although conventional analysis techniques are effective, they are time-consuming and laborious, which has produced a substantial backlog of casework samples to be analyzed. Microfluidic technologies present one solution to this problem. The work presented describes techniques aimed at improving forensic genetic analysis techniques, and specifically addresses procedures necessary for sexual assault evidence analysis, where male and female DNA must be separately interrogated.
520 $a Sexual assault evidence typically contains a mixture of sperm cells from the perpetrator in an excess of epithelial cells from the victim; efficient recovery of cells from the cotton swab, as well as isolation of sperm cells from female DNA, are required for analysis. Conventional methods for recovery and separation of the genetic material are time-consuming and often inefficient. In an effort to improve cell recovery over conventional methods, two procedures were developed and are described; the first incorporates enzymes for digestion of the cotton matrix, while the second includes detergents for the release of cells from cotton, and proteolytic digestion for preferential lysis of epithelial cells. These methods can be used to supplant conventional macroscale techniques, or in conjunction with microchip cell separation methods.
520 $a Microchip technology offers the potential of rapid, cost-effective alternatives to conventional DNA analysis methods. Techniques performed on microdevices are particularly advantageous because they can be integrated with downstream analytical steps on a single microfluidic device in the form of a micro-total analysis system (muTAS). Implementation of integrated systems for forensic DNA analysis will reduce analysis times, and, therefore, the forensic casework backlog. The presented work also details the development of an integrated microdevice for sedimentation-based cell sorting of sperm and epithelial cells, and independent silica-based solid phase extraction of the two cell types. In addition, the development of an alternative cell separation method, based on capture of cells using acoustic trapping, is presented. The work presented represents major steps towards the development of a fully integrated microdevice capable of total DNA analysis for forensic casework.
590 $a School code: 0246.
650 4 $a Anthropology, Medical and Forensic. $3 1020279
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0339
690 $a 0486
710 2 $a University of Virginia. $3 645578
773 0 $t Dissertation Abstracts International $g 70-02B.
790 $a 0246
790 1 0 $a Landers, James P., $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3348723