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Retinal pigment epithelial cells and...

The University of Alabama at Birmingham.

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Retinal pigment epithelial cells and the insulin-like growth factor system in proliferative vitreoretinopathy.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Retinal pigment epithelial cells and the insulin-like growth factor system in proliferative vitreoretinopathy./
Author:	Mukherjee, Sudipto.
Description:	75 p.
Notes:	Adviser: Clyde Guidry.
Contained By:	Dissertation Abstracts International68-10B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3286390
ISBN:	9780549289852

Retinal pigment epithelial cells and the insulin-like growth factor system in proliferative vitreoretinopathy.
Mukherjee, Sudipto.

Retinal pigment epithelial cells and the insulin-like growth factor system in proliferative vitreoretinopathy. - 75 p.

Adviser: Clyde Guidry.

Thesis (Ph.D.)--The University of Alabama at Birmingham, 2007.

The goal of this study was to determine the influence, if any, of the insulin-like growth factors (IGFs) on retinal pigment epithelial (RPE) cell tractional force generation and the contributions of vitreous insulin-like growth factor binding proteins (IGFBPs) towards control of IGF activity. Another objective was to evaluate RPE cells as a potential source of all six high-affinity IGFBPs and to determine if IGFBP biosynthesis is modulated in concert with phenotype changes and growth factor stimuli known to be present in disease.

ISBN: 9780549289852Subjects--Topical Terms:

1017686
Biology, Cell.

Retinal pigment epithelial cells and the insulin-like growth factor system in proliferative vitreoretinopathy.
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020 $a 9780549289852
035 $a (UMI)AAI3286390
035 $a AAI3286390
040 $a UMI $c UMI
100 1 $a Mukherjee, Sudipto. $3 1025211
245 1 0 $a Retinal pigment epithelial cells and the insulin-like growth factor system in proliferative vitreoretinopathy.
300 $a 75 p.
500 $a Adviser: Clyde Guidry.
500 $a Source: Dissertation Abstracts International, Volume: 68-10, Section: B, page: 6430.
502 $a Thesis (Ph.D.)--The University of Alabama at Birmingham, 2007.
520 $a The goal of this study was to determine the influence, if any, of the insulin-like growth factors (IGFs) on retinal pigment epithelial (RPE) cell tractional force generation and the contributions of vitreous insulin-like growth factor binding proteins (IGFBPs) towards control of IGF activity. Another objective was to evaluate RPE cells as a potential source of all six high-affinity IGFBPs and to determine if IGFBP biosynthesis is modulated in concert with phenotype changes and growth factor stimuli known to be present in disease.
520 $a RPE generate tractional forces in response to IGF-I and -II with IGF-I being the more potent stimulus. Differential RPE responses to non-IGFBP binding analogue, R3IGF-I reflected minor amounts of endogenous IGFBP production. IGFBPs -2, -3 and -5 were effective inhibitors of both ligands while IGFBP-6 reduced cell responses to IGF-II only. IGFBP direct effects on the cells were binding protein-specific in that only IGFBP-1 had detectable stimulatory effects and IGFBPs -3, -4, -5 and -6 inhibited RPE responses.
520 $a Changes in RPE phenotype occur very early in culture and can be defined by differential expression of cytoskeletal proteins. Normal RPE are immunoreactive for cytokeratin 18 and negative for cytokeratin 19, vimentin and alpha smooth muscle actin (alphaSMA). At seven days (7d) in culture, RPE cells express cytokeratins 18, 19 and vimentin. After 35 days (35d) in continuously proliferating cultures, RPE cells express cytokeratin 19, vimentin and alphaSMA. RT-PCR studies revealed that normal RPE express IGFBP-2 -3, -4, -5 and -6, but not IGFBP-1. Following introduction in to culture, 'early reactive ' (7d) and 'myofibroblastic' (35d) RPE possess detectable message for IGFBP-3, -5 and -6. However, Northern, Western ligand and Western blots suggest that functional IGFBP production by these RPE is limited to IGFBP-5 and this secretory profile is not under the influence of IGF system ligands. These data provide compelling evidence that RPE cells produce as well as respond to IGF system components, signals that drive the fibrocontractive process in proliferative vitreoretinopathy.
590 $a School code: 0005.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Health Sciences, Ophthalmology. $3 1019445
690 $a 0379
690 $a 0381
710 2 $a The University of Alabama at Birmingham. $3 1019443
773 0 $t Dissertation Abstracts International $g 68-10B.
790 $a 0005
790 1 0 $a Guidry, Clyde, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3286390