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The alpha(2)beta(1) integrin: Cloni...

Washington University in St. Louis.

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The alpha(2)beta(1) integrin: Cloning, expression, and roles in development.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The alpha(2)beta(1) integrin: Cloning, expression, and roles in development./
Author:	Wu, Justina Eng Hui.
Description:	239 p.
Notes:	Chairperson: Samuel A. Santoro.
Contained By:	Dissertation Abstracts International58-04B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=9730990
ISBN:	9780591403886

The alpha(2)beta(1) integrin: Cloning, expression, and roles in development.
Wu, Justina Eng Hui.

The alpha(2)beta(1) integrin: Cloning, expression, and roles in development. - 239 p.

Chairperson: Samuel A. Santoro.

Thesis (Ph.D.)--Washington University in St. Louis, 1997.

Embryogenesis requires appropriate interactions between cells and their microenvironment which is composed largely of the extracellular matrix (ECM). Collagens and laminins, two major components of the ECM, are known to influence or direct multiple developmental processes such as cell differentiation, cell migration, neurite outgrowth, and tubular morphogenesis. Many of the interaction between the cell and the collagen/laminin matrix is mediated by $\alpha\sb2\beta\sb1$, a member of the integrin family of adhesion molecules. The experiments presented in the thesis were done to study the integrin during $\alpha\sb2\beta\sb1$ integrin during mouse development. Specifically, the mouse homologue of the $\alpha\sb2$ subunit was cloned and sequenced which provided a molecular probe for $\alpha\sb2$ mRNA. In addition, a polyclonal antibody raised against a peptide identical to the carboxy terminal of mouse $\alpha\sb2$ was generated. With the molecular probe and the polyclonal antibody specific to $\alpha\sb2$, the spatial and temporal expression pattern of the subunit during mouse embryonic and fetal development was elucidated. The observed expression of the $\alpha\sb2$ integrin subunit on cells that were actively assembling a collagen/laminin matrix or undergoing cell/tissue migration suggested a possible role for the $\alpha\sb2\beta\sb1$ integrin in these processes. In order to study these roles, in vitro organ culture systems for both the lung and limb cartilage were characterized for their ability to recapitulate in vivo organogenesis. An attempt to study the effects of aberrantly decreasing or constitutively expressing the $\alpha\sb2\beta\sb1$ integrin subunit in these culture systems was done by using $\alpha\sb2$ and $\beta\sb1$ specific antisense oligonucleotide and retroviral vectors carrying sense and antisense $\alpha\sb2$ cDNA.

ISBN: 9780591403886Subjects--Topical Terms:

1017686
Biology, Cell.

The alpha(2)beta(1) integrin: Cloning, expression, and roles in development.
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020 $a 9780591403886
035 $a (UMI)AAI9730990
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100 1 $a Wu, Justina Eng Hui. $3 1025072
245 1 4 $a The alpha(2)beta(1) integrin: Cloning, expression, and roles in development.
300 $a 239 p.
500 $a Chairperson: Samuel A. Santoro.
500 $a Source: Dissertation Abstracts International, Volume: 58-04, Section: B, page: 1642.
502 $a Thesis (Ph.D.)--Washington University in St. Louis, 1997.
520 $a Embryogenesis requires appropriate interactions between cells and their microenvironment which is composed largely of the extracellular matrix (ECM). Collagens and laminins, two major components of the ECM, are known to influence or direct multiple developmental processes such as cell differentiation, cell migration, neurite outgrowth, and tubular morphogenesis. Many of the interaction between the cell and the collagen/laminin matrix is mediated by $\alpha\sb2\beta\sb1$, a member of the integrin family of adhesion molecules. The experiments presented in the thesis were done to study the integrin during $\alpha\sb2\beta\sb1$ integrin during mouse development. Specifically, the mouse homologue of the $\alpha\sb2$ subunit was cloned and sequenced which provided a molecular probe for $\alpha\sb2$ mRNA. In addition, a polyclonal antibody raised against a peptide identical to the carboxy terminal of mouse $\alpha\sb2$ was generated. With the molecular probe and the polyclonal antibody specific to $\alpha\sb2$, the spatial and temporal expression pattern of the subunit during mouse embryonic and fetal development was elucidated. The observed expression of the $\alpha\sb2$ integrin subunit on cells that were actively assembling a collagen/laminin matrix or undergoing cell/tissue migration suggested a possible role for the $\alpha\sb2\beta\sb1$ integrin in these processes. In order to study these roles, in vitro organ culture systems for both the lung and limb cartilage were characterized for their ability to recapitulate in vivo organogenesis. An attempt to study the effects of aberrantly decreasing or constitutively expressing the $\alpha\sb2\beta\sb1$ integrin subunit in these culture systems was done by using $\alpha\sb2$ and $\beta\sb1$ specific antisense oligonucleotide and retroviral vectors carrying sense and antisense $\alpha\sb2$ cDNA.
590 $a School code: 0252.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Zoology. $3 1018632
690 $a 0379
690 $a 0472
710 2 $a Washington University in St. Louis. $3 1017519
773 0 $t Dissertation Abstracts International $g 58-04B.
790 $a 0252
790 1 0 $a Santoro, Samuel A., $e advisor
791 $a Ph.D.
792 $a 1997
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=9730990