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Effects of host specificity on Esche...

Wayne State University., Physiology.

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Effects of host specificity on Escherichia coli chemotaxis behavior, chemotaxis genes and chemotaxis protein expression.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Effects of host specificity on Escherichia coli chemotaxis behavior, chemotaxis genes and chemotaxis protein expression./
Author:	Dzinic, Sijana H.
Description:	127 p.
Notes:	Adviser: Jeffrey Ram.
Contained By:	Dissertation Abstracts International69-03B.
Subject:	Biology, Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3298000
ISBN:	9780549498865

Effects of host specificity on Escherichia coli chemotaxis behavior, chemotaxis genes and chemotaxis protein expression.
Dzinic, Sijana H.

Effects of host specificity on Escherichia coli chemotaxis behavior, chemotaxis genes and chemotaxis protein expression. - 127 p.

Adviser: Jeffrey Ram.

Thesis (Ph.D.)--Wayne State University, 2008.

Genes involved in chemotaxis may be important in establishing the commensal relationships of enteric bacteria in warm-blooded animals. This study investigates the hypothesis that host-specific associations of E. coli are partially mediated by differences in chemotaxis responses to different host diets. The hypothesis was tested by investigating the chemotactic behavior of wild E. coli strains (isolated from carnivores, herbivores, and omnivores) employing swarm plate and capillary chemotaxis assays. Growth assays examined carbon source utilization on chemotaxis results.

ISBN: 9780549498865Subjects--Topical Terms:

1017816
Biology, Physiology.

Effects of host specificity on Escherichia coli chemotaxis behavior, chemotaxis genes and chemotaxis protein expression.
LDR:03646nam 2200361 a 45 001 856661
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020 $a 9780549498865
035 $a (UMI)AAI3298000
035 $a AAI3298000
040 $a UMI $c UMI
100 1 $a Dzinic, Sijana H. $3 1023499
245 1 0 $a Effects of host specificity on Escherichia coli chemotaxis behavior, chemotaxis genes and chemotaxis protein expression.
300 $a 127 p.
500 $a Adviser: Jeffrey Ram.
500 $a Source: Dissertation Abstracts International, Volume: 69-03, Section: B, page: 1498.
502 $a Thesis (Ph.D.)--Wayne State University, 2008.
520 $a Genes involved in chemotaxis may be important in establishing the commensal relationships of enteric bacteria in warm-blooded animals. This study investigates the hypothesis that host-specific associations of E. coli are partially mediated by differences in chemotaxis responses to different host diets. The hypothesis was tested by investigating the chemotactic behavior of wild E. coli strains (isolated from carnivores, herbivores, and omnivores) employing swarm plate and capillary chemotaxis assays. Growth assays examined carbon source utilization on chemotaxis results.
520 $a Chemotaxis assays showed that strains isolated from carnivores "preferred" the aspartate to serine, while strains isolated from herbivores were attracted to both chemicals equally or "preferred" serine to aspartate. Strains from humans behaved similarly to strains from herbivores.
520 $a Analysis of DNA sequences of chemotaxis genes tar and tsr, and examination of amino acid sequences of their respective proteinsTar (aspartate receptor) and Tsr (serine receptor) revealed mostly synonymous polymorphic sites. Although CAI for tsr and tar varied significantly by a few percent between strains from carnivores and herbivores, the change in expression that this would cause is unlikely to be biologically significant. A few polymorphisms resulting in amino acid changes might cause chemotactic differences but were not significantly different in frequency between carnivore and herbivore groups of E. coli.
520 $a Variable numbers of tandem 9-nucleotide insertions coding for polyQ sequences were discovered in tsr from different strains (similar to human polyQ genetic diseases such as Huntington's disease). These variable nucleotide insertions had no effect on bacterial chemotaxis.
520 $a RT-PCR and immunoblots were used to determine whether changes in Tar and Tsr expression might explain chemotaxis differences between strains from carnivores and herbivores. The ratios of tar:tsr mRNA and Tar:Tsr protein showed strong trends towards higher values in strains from carnivores compared to herbivores, with p values fluctuating around p = 0.05. The ratio of Tar:Tsr protein was correlated with relative chemotaxis towards aspartate and serine in capillary chemotaxis assays (R2 = 0.55; p = 0.001). The strong trends in these data thus supported the hypothesis that differences in methyl-accepting chemotaxis protein expression could produce the differences in chemotaxis between strains from carnivores and herbivores.
590 $a School code: 0254.
650 4 $a Biology, Physiology. $3 1017816
690 $a 0719
710 2 $a Wayne State University. $b Physiology. $3 1023498
773 0 $t Dissertation Abstracts International $g 69-03B.
790 $a 0254
790 1 0 $a Cala, Steven $e committee member
790 1 0 $a Dunbar, Joseph C. $e committee member
790 1 0 $a Kapatos, Gregory $e committee member
790 1 0 $a Ram, Jeffrey, $e advisor
790 1 0 $a Rillema, James A. $e committee member
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3298000