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Evaluation of NIR, FTIR and Raman sp...

University of Puerto Rico, Mayaguez (Puerto Rico).

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Evaluation of NIR, FTIR and Raman spectroscopies as monitoring techniques for recombinant GFP protein production in fed-batch cultures of Escherichia coli.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Evaluation of NIR, FTIR and Raman spectroscopies as monitoring techniques for recombinant GFP protein production in fed-batch cultures of Escherichia coli./
Author:	Rosario Rosario, Arnaldo Jose.
Description:	94 p.
Notes:	Adviser: Lorenzo Saliceti Piazza.
Contained By:	Masters Abstracts International47-02.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1458838
ISBN:	9780549798446

Evaluation of NIR, FTIR and Raman spectroscopies as monitoring techniques for recombinant GFP protein production in fed-batch cultures of Escherichia coli.
Rosario Rosario, Arnaldo Jose.

Evaluation of NIR, FTIR and Raman spectroscopies as monitoring techniques for recombinant GFP protein production in fed-batch cultures of Escherichia coli. - 94 p.

Adviser: Lorenzo Saliceti Piazza.

Thesis (M.S.)--University of Puerto Rico, Mayaguez (Puerto Rico), 2009.

Although on-line automated process information about fermentation biomass, substrates and products is required to improve the monitoring techniques such as process optimization and achieve a better understanding of the process. The significant and vital information is limited for to date processes. While many chemical and physical measurements are measurable at-line, others such as the concentration of analytes are difficult to determine and often required considerable pre-processing of the samples. Chemometrics use the analytical techniques such as infrared spectroscopy and multivariable statistic to offers the opportunity to improve the fermentation processes by incorporating rapid, nondestructive, and multi-constituent analysis of fermentation broth media.

ISBN: 9780549798446Subjects--Topical Terms:

586156
Chemistry, Analytical.

Evaluation of NIR, FTIR and Raman spectroscopies as monitoring techniques for recombinant GFP protein production in fed-batch cultures of Escherichia coli.
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020 $a 9780549798446
035 $a (UMI)AAI1458838
035 $a AAI1458838
040 $a UMI $c UMI
100 1 $a Rosario Rosario, Arnaldo Jose. $3 1022457
245 1 0 $a Evaluation of NIR, FTIR and Raman spectroscopies as monitoring techniques for recombinant GFP protein production in fed-batch cultures of Escherichia coli.
300 $a 94 p.
500 $a Adviser: Lorenzo Saliceti Piazza.
500 $a Source: Masters Abstracts International, Volume: 47-02, page: 1083.
502 $a Thesis (M.S.)--University of Puerto Rico, Mayaguez (Puerto Rico), 2009.
520 $a Although on-line automated process information about fermentation biomass, substrates and products is required to improve the monitoring techniques such as process optimization and achieve a better understanding of the process. The significant and vital information is limited for to date processes. While many chemical and physical measurements are measurable at-line, others such as the concentration of analytes are difficult to determine and often required considerable pre-processing of the samples. Chemometrics use the analytical techniques such as infrared spectroscopy and multivariable statistic to offers the opportunity to improve the fermentation processes by incorporating rapid, nondestructive, and multi-constituent analysis of fermentation broth media.
520 $a Our main focus is to assess and correlate the NIR, IR and Raman spectra with multivariate techniques such as Partial Least Squares (PLS) to monitor the critical variables in a microbial fermentation such as recombinant protein synthesis, analyte and biomass concentrations.
590 $a School code: 0553.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Engineering, Chemical. $3 1018531
690 $a 0486
690 $a 0542
710 2 $a University of Puerto Rico, Mayaguez (Puerto Rico). $3 1017811
773 0 $t Masters Abstracts International $g 47-02.
790 $a 0553
790 1 0 $a Saliceti Piazza, Lorenzo, $e advisor
791 $a M.S.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1458838