東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Coupling in vivo microdialysis sampl...

The University of Wisconsin - Madison.

Linked to FindBook

Google Book

Amazon

博客來

Coupling in vivo microdialysis sampling to mass spectrometry to detect neuropeptides in a decapod crustacean.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Coupling in vivo microdialysis sampling to mass spectrometry to detect neuropeptides in a decapod crustacean./
Author:	Behrens, Heidi Leigh.
Description:	116 p.
Notes:	Adviser: Lingjun Li.
Contained By:	Dissertation Abstracts International69-09B.
Subject:	Biology, Animal Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3327782
ISBN:	9780549802839

Coupling in vivo microdialysis sampling to mass spectrometry to detect neuropeptides in a decapod crustacean.
Behrens, Heidi Leigh.

Coupling in vivo microdialysis sampling to mass spectrometry to detect neuropeptides in a decapod crustacean. - 116 p.

Adviser: Lingjun Li.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2008.

Neuropeptides are important chemical messengers in the nervous system and can act as neurotransmitters, neuromodulators, or neurohormones. Decapod crustaceans are important model organisms for studying how peptide hormones regulate physiological processes. This project employed a unique sampling and detection methodology to study neurohormones in the crab, Cancer borealis. An in vivo microdialysis sampling technique was developed and coupled with capillary liquid chromatography mass spectrometry (MS) as well as matrix-assisted laser desorption ionization MS. This sampling and detection scheme resulted in the detection of many known neuropeptide masses in crab hemolymph, as well as the tandem MS sequencing of three known neuropeptides and one novel peptide. A comparison of microdialysis sampling with typical hemolymph preparation methods revealed more neuropeptides and fewer protein fragments in samples collected via microdialysis. This study demonstrates the ability of microdialysis to collect clean, neuropeptide-rich samples from the hemolymph of a live crab that can be detected using MS analysis. This method can easily be applied to detect neuropeptides or other low-mass analytes from other crustacean model organisms and complements current tissue-based techniques for sampling neuropeptides. As an in vivo sampling method, microdialysis with MS detection has the potential for real-time analysis of neuropeptides in a live, freely-moving organism.

ISBN: 9780549802839Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Coupling in vivo microdialysis sampling to mass spectrometry to detect neuropeptides in a decapod crustacean.
LDR:02432nam 2200301 a 45 001 855228
005 20100708
008 100708s2008 ||||||||||||||||| ||eng d
020 $a 9780549802839
035 $a (UMI)AAI3327782
035 $a AAI3327782
040 $a UMI $c UMI
100 1 $a Behrens, Heidi Leigh. $3 1021783
245 1 0 $a Coupling in vivo microdialysis sampling to mass spectrometry to detect neuropeptides in a decapod crustacean.
300 $a 116 p.
500 $a Adviser: Lingjun Li.
500 $a Source: Dissertation Abstracts International, Volume: 69-09, Section: B, page: 5365.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2008.
520 $a Neuropeptides are important chemical messengers in the nervous system and can act as neurotransmitters, neuromodulators, or neurohormones. Decapod crustaceans are important model organisms for studying how peptide hormones regulate physiological processes. This project employed a unique sampling and detection methodology to study neurohormones in the crab, Cancer borealis. An in vivo microdialysis sampling technique was developed and coupled with capillary liquid chromatography mass spectrometry (MS) as well as matrix-assisted laser desorption ionization MS. This sampling and detection scheme resulted in the detection of many known neuropeptide masses in crab hemolymph, as well as the tandem MS sequencing of three known neuropeptides and one novel peptide. A comparison of microdialysis sampling with typical hemolymph preparation methods revealed more neuropeptides and fewer protein fragments in samples collected via microdialysis. This study demonstrates the ability of microdialysis to collect clean, neuropeptide-rich samples from the hemolymph of a live crab that can be detected using MS analysis. This method can easily be applied to detect neuropeptides or other low-mass analytes from other crustacean model organisms and complements current tissue-based techniques for sampling neuropeptides. As an in vivo sampling method, microdialysis with MS detection has the potential for real-time analysis of neuropeptides in a live, freely-moving organism.
590 $a School code: 0262.
650 4 $a Biology, Animal Physiology. $3 1017835
650 4 $a Biology, Neuroscience. $3 1017680
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0317
690 $a 0433
690 $a 0486
690 $a 0487
710 2 $a The University of Wisconsin - Madison. $3 626640
773 0 $t Dissertation Abstracts International $g 69-09B.
790 $a 0262
790 1 0 $a Li, Lingjun, $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3327782