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On the nature of aqueous solvation i...

University of Pennsylvania.

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On the nature of aqueous solvation in biological systems.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	On the nature of aqueous solvation in biological systems./
Author:	Nucci, Nathaniel V.
Description:	236 p.
Notes:	Source: Dissertation Abstracts International, Volume: 69-04, Section: B, page: 2308.
Contained By:	Dissertation Abstracts International69-04B.
Subject:	Biophysics, Medical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3309488
ISBN:	9780549577690

On the nature of aqueous solvation in biological systems.
Nucci, Nathaniel V.

On the nature of aqueous solvation in biological systems. - 236 p.

Source: Dissertation Abstracts International, Volume: 69-04, Section: B, page: 2308.

Thesis (Ph.D.)--University of Pennsylvania, 2008.

The properties and behavior of water greatly influence biomolecular structure and function, yet many of the subtle effects of water near biomolecular surfaces remain very difficult to study experimentally. The goal of the present work was to develop methodologies to experimentally analyze the water hydrogen bond network near biological molecules and to examine whether the tale of biomolecular solvation was fully encoded in the behavior of this network. An effective two-state hydrogen bonding model was applied to interpret the O-H stretching infrared absorption of water in terms of a mixture of linear and bent hydrogen bonds. Using this simplified model to quantitatively compare temperature excursion infrared spectra of water under various conditions, the effects of ions and nanoconfinement on the water hydrogen bond network were described. Salts were shown to alter the hydrogen bond network behavior of water in correlation with the positions of their constituent ions in the Hofmeister series. Nanoconfinement in reverse micelles was shown to drastically reduce the temperature-sensitivity of the water hydrogen bond network. Under these nanoconfined conditions, bulk water behavior was seen only for a water pool of approximately 15 nm in diameter or larger. These studies indicate that the majority of intracellular water is greatly influenced by ions and charged biomolecular surfaces, and that such water does not behave as bulk water. Water structure near biological small molecules was also examined, and no effect was seen in the influence of solute on solvent for single functional group changes in the small molecules. Despite the lack of difference in the water surrounding these various molecules, the differential coupling of one of their complex aromatic ring vibrational modes to solvent was demonstrated. This coupling was shown to be mediated by hydrogen bonding between peripheral substituents and solvent and was found to depend on the nature and location of hydrogen-bonding substitutions. These findings, taken within the context of other recent work examining the nature of solvation in biological systems, produce a picture of the intracellular environment such that extensive vibrational coupling is possible. The functional implications of such coupling remain to be determined by future investigations.

ISBN: 9780549577690Subjects--Topical Terms:

1017681
Biophysics, Medical.

On the nature of aqueous solvation in biological systems.
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035 $a (UMI)AAI3309488
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100 1 $a Nucci, Nathaniel V. $3 1019126
245 1 0 $a On the nature of aqueous solvation in biological systems.
300 $a 236 p.
500 $a Source: Dissertation Abstracts International, Volume: 69-04, Section: B, page: 2308.
502 $a Thesis (Ph.D.)--University of Pennsylvania, 2008.
520 $a The properties and behavior of water greatly influence biomolecular structure and function, yet many of the subtle effects of water near biomolecular surfaces remain very difficult to study experimentally. The goal of the present work was to develop methodologies to experimentally analyze the water hydrogen bond network near biological molecules and to examine whether the tale of biomolecular solvation was fully encoded in the behavior of this network. An effective two-state hydrogen bonding model was applied to interpret the O-H stretching infrared absorption of water in terms of a mixture of linear and bent hydrogen bonds. Using this simplified model to quantitatively compare temperature excursion infrared spectra of water under various conditions, the effects of ions and nanoconfinement on the water hydrogen bond network were described. Salts were shown to alter the hydrogen bond network behavior of water in correlation with the positions of their constituent ions in the Hofmeister series. Nanoconfinement in reverse micelles was shown to drastically reduce the temperature-sensitivity of the water hydrogen bond network. Under these nanoconfined conditions, bulk water behavior was seen only for a water pool of approximately 15 nm in diameter or larger. These studies indicate that the majority of intracellular water is greatly influenced by ions and charged biomolecular surfaces, and that such water does not behave as bulk water. Water structure near biological small molecules was also examined, and no effect was seen in the influence of solute on solvent for single functional group changes in the small molecules. Despite the lack of difference in the water surrounding these various molecules, the differential coupling of one of their complex aromatic ring vibrational modes to solvent was demonstrated. This coupling was shown to be mediated by hydrogen bonding between peripheral substituents and solvent and was found to depend on the nature and location of hydrogen-bonding substitutions. These findings, taken within the context of other recent work examining the nature of solvation in biological systems, produce a picture of the intracellular environment such that extensive vibrational coupling is possible. The functional implications of such coupling remain to be determined by future investigations.
590 $a School code: 0175.
650 4 $a Biophysics, Medical. $3 1017681
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Physical. $3 560527
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690 $a 0494
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710 2 $a University of Pennsylvania. $3 1017401
773 0 $t Dissertation Abstracts International $g 69-04B.
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792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3309488