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The proliferative response of equine...

Clemson University.

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The proliferative response of equine chondrocytes to bovine lymph node proteins in vitro.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The proliferative response of equine chondrocytes to bovine lymph node proteins in vitro./
Author:	Reed, Alison Marie.
Description:	70 p.
Notes:	Adviser: Steven E. Ellis.
Contained By:	Masters Abstracts International45-04.
Subject:	Biology, Animal Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1441633

The proliferative response of equine chondrocytes to bovine lymph node proteins in vitro.
Reed, Alison Marie.

The proliferative response of equine chondrocytes to bovine lymph node proteins in vitro. - 70 p.

Adviser: Steven E. Ellis.

Thesis (M.S.)--Clemson University, 2007.

Lameness poses a substantial challenge to the horse industry, costing millions of dollars per year in treatments, labor, and loss of use of performance horses. Osteoarthritis is a leading cause of chronic lameness. Treatments that stimulate cartilage development may promote cartilage healing. Many current treatment options for osteoarthritis have limited effectiveness and are expensive. Bovine supramammary lymph nodes are widely available at no cost and contain bioactive growth factors that could promote cartilage healing. The purpose of this study was to determine the proliferative effect of bovine lymph node extract on equine chondrocytes in vitro.Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

The proliferative response of equine chondrocytes to bovine lymph node proteins in vitro.
LDR:02948nam 2200301 a 45 001 852109
005 20100629
008 100629s2007 ||||||||||||||||| ||eng d
035 $a (UMI)AAI1441633
035 $a AAI1441633
040 $a UMI $c UMI
100 1 $a Reed, Alison Marie. $3 1017834
245 1 4 $a The proliferative response of equine chondrocytes to bovine lymph node proteins in vitro.
300 $a 70 p.
500 $a Adviser: Steven E. Ellis.
500 $a Source: Masters Abstracts International, Volume: 45-04, page: 1846.
502 $a Thesis (M.S.)--Clemson University, 2007.
520 $a Lameness poses a substantial challenge to the horse industry, costing millions of dollars per year in treatments, labor, and loss of use of performance horses. Osteoarthritis is a leading cause of chronic lameness. Treatments that stimulate cartilage development may promote cartilage healing. Many current treatment options for osteoarthritis have limited effectiveness and are expensive. Bovine supramammary lymph nodes are widely available at no cost and contain bioactive growth factors that could promote cartilage healing. The purpose of this study was to determine the proliferative effect of bovine lymph node extract on equine chondrocytes in vitro.
520 $a Equine chondrocytes were harvested from the scapular cartilage of adult horses. Lymph node extract (LNE) was prepared by mixing freeze-dried lymph node powder with PBS followed by heat inactivation. Chondrocytes were cultured in vitro, first in DMEM with 10% bovine growth serum (BGS) and antibiotics, then with treatment media containing either BGS or LNE on equal and unequal protein bases. Additionally, LNE fractions from ammonium sulfate precipitation were examined as BGS replacements. The response of the cells to IGF-I and TGF-beta was also examined. DNA accumulation was measured using the CyQUANT DNA assay as a means to quantify cell stimulation, i.e. growth.
520 $a When BGS and LNE were compared on equal and unequal protein basis over a range of protein concentrations, there were no statistically significant differences observed in proliferation between BGS- and LNE- treated cells. However, in a separate experiment, cells treated with LNE had significantly higher cell proliferation than cells treated with BGS or different LNE fractions. IGF-I and TGF-beta had no significant effects on cell proliferation.
520 $a Overall, the results indicate LNE is capable of stimulating chondrocyte proliferation to an equal or greater degree than BGS, suggesting LNE could promote cartilage healing. Further studies are needed to determine if LNE can be used to develop new therapies for osteoarthritis in horses.
590 $a School code: 0050.
650 4 $a Biology, Animal Physiology. $3 1017835
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0433
690 $a 0487
710 2 $a Clemson University. $3 997173
773 0 $t Masters Abstracts International $g 45-04.
790 $a 0050
790 1 0 $a Ellis, Steven E., $e advisor
791 $a M.S.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1441633