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Engineering Protein Tools to Underst...

Lui, Irene,

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Engineering Protein Tools to Understand Changes in Cell Surface Proteolysis and Viral Protein-Protein Interactions /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Engineering Protein Tools to Understand Changes in Cell Surface Proteolysis and Viral Protein-Protein Interactions // Irene Lui.
Author:	Lui, Irene,
Description:	1 electronic resource (125 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-09, Section: B.
Contained By:	Dissertations Abstracts International85-09B.
Subject:	Biochemistry. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30995304
ISBN:	9798381971668

Engineering Protein Tools to Understand Changes in Cell Surface Proteolysis and Viral Protein-Protein Interactions /
Lui, Irene,

Engineering Protein Tools to Understand Changes in Cell Surface Proteolysis and Viral Protein-Protein Interactions /Irene Lui. - 1 electronic resource (125 pages)

Source: Dissertations Abstracts International, Volume: 85-09, Section: B.

Cell surface proteolysis plays a crucial role in regulating various biological and cellular processes, including but not limited to cell signaling, protein maturation, and extracellular matrix remodeling. Dysregulation of these proteolytic events is often a hallmark of numerous diseases. Understanding these changes in proteolysis may present opportunities for therapeutic intervention as neo-epitopes are created that may be preferentially displayed in diseased states. Additionally, it is important to understand the mechanism of action for protein-protein interactions in disease, particularly in the case of viral infection, as these interactions may be therapeutic targets for disruption. The following work describes the development of novel protein tools to understand how cell surface proteolysis changes when various oncogenes are overexpressed in breast cancer and how SARS-CoV-2 Spike protein interacts with the human ACE2 receptor. In Chapter 1, I describe the work done to develop a method utilizing subtiligase to study changes to the proteolytic landscape of MCF10A cells under the influence of KRAS G12V and Her2 overexpression. Chapter 2 describes the work done to study changes in cell surface proteolysis and protein secretion in pancreatic ductal adenocarcinoma under hypoxic conditions. In Chapter 3, I describe the develop of various protein-Fc fusions to study the protein-protein interactions of Spike protein and ACE2.

English

ISBN: 9798381971668Subjects--Topical Terms:

518028
Biochemistry.
Subjects--Index Terms:

Hypoxia

Engineering Protein Tools to Understand Changes in Cell Surface Proteolysis and Viral Protein-Protein Interactions /
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500 $a Source: Dissertations Abstracts International, Volume: 85-09, Section: B.
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520 $a Cell surface proteolysis plays a crucial role in regulating various biological and cellular processes, including but not limited to cell signaling, protein maturation, and extracellular matrix remodeling. Dysregulation of these proteolytic events is often a hallmark of numerous diseases. Understanding these changes in proteolysis may present opportunities for therapeutic intervention as neo-epitopes are created that may be preferentially displayed in diseased states. Additionally, it is important to understand the mechanism of action for protein-protein interactions in disease, particularly in the case of viral infection, as these interactions may be therapeutic targets for disruption. The following work describes the development of novel protein tools to understand how cell surface proteolysis changes when various oncogenes are overexpressed in breast cancer and how SARS-CoV-2 Spike protein interacts with the human ACE2 receptor. In Chapter 1, I describe the work done to develop a method utilizing subtiligase to study changes to the proteolytic landscape of MCF10A cells under the influence of KRAS G12V and Her2 overexpression. Chapter 2 describes the work done to study changes in cell surface proteolysis and protein secretion in pancreatic ductal adenocarcinoma under hypoxic conditions. In Chapter 3, I describe the develop of various protein-Fc fusions to study the protein-protein interactions of Spike protein and ACE2.
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650 4 $a Molecular biology. $3 517296
650 4 $a Cellular biology. $3 3172791
650 4 $a Bioinformatics. $3 553671
650 4 $a Analytical chemistry. $3 3168300
653 $a Hypoxia
653 $a Mass spectrometry
653 $a Pancreatic ductal adenocarcinoma
653 $a Protein engineering
653 $a Proteolysis
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710 2 $a University of California, San Francisco. $b Biochemistry and Molecular Biology. $e degree granting institution. $3 3830182
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