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Determining the intracellular concen...

Pham, Anh Dang,

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Determining the intracellular concentrations of flavonoids in MDA-MB-231 cells using HPLC-coupled mass spectrometry /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Determining the intracellular concentrations of flavonoids in MDA-MB-231 cells using HPLC-coupled mass spectrometry // Anh Dang Pham.
Author:	Pham, Anh Dang,
Description:	1 electronic resource (68 pages)
Notes:	Source: Masters Abstracts International, Volume: 74-09.
Contained By:	Masters Abstracts International74-09.
Subject:	Molecular biology. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1533038
ISBN:	9781267901125

Determining the intracellular concentrations of flavonoids in MDA-MB-231 cells using HPLC-coupled mass spectrometry /
Pham, Anh Dang,

Determining the intracellular concentrations of flavonoids in MDA-MB-231 cells using HPLC-coupled mass spectrometry /Anh Dang Pham. - 1 electronic resource (68 pages)

Source: Masters Abstracts International, Volume: 74-09.

Flavonoids are polyphenolic compounds found in a wide variety of plants. In recent years, flavonoids have been found to be beneficial to human health. Our lab has investigated the ability of flavonoids to induce cytotoxicity in human breast cancer cell lines. We have discovered that some compounds can induce cytotoxicity very readily, whereas others cannot. We hypothesized that this difference is attributable to a compound's ability to transverse the cell membrane. Therefore, we used LC-MS to measure the amount of each flavonoid that is able to enter into MDA-MB-231 cells, human breast cancer cells. We tested 14 flavonoids. MDA-MB-231 cells were plated and treated with 100 μM flavonoids for 24 and 48 hr. After treatment, cells were extracted using methanol. Flavonoids from extracts were characterized and quantitated by LC-MS. We found that intracellular flavonoid concentration increased over time for some of the flavonoids. We could not detect quercetin, myricetin, and catechin in cell extracts. However, quercetin was found to be a moderate inducer of cytotoxicity, whereas myricetin and catechin were not. We found that quercetin dimerized over time in the cell medium only in the presence of serum. The quercetin dimer could not be detected in cellular extracts, which suggested that it was unable to cross the membrane. Additionally, synthetic quercetin dimer did not induce cell death. Our results suggest that only fresh quercetin killed cells; once quercetin dimerized, it could not induce cell death.

English

ISBN: 9781267901125Subjects--Topical Terms:

517296
Molecular biology.
Subjects--Index Terms:

HPLC

Determining the intracellular concentrations of flavonoids in MDA-MB-231 cells using HPLC-coupled mass spectrometry /
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100 1 $a Pham, Anh Dang, $e author. $3 3829231
245 1 0 $a Determining the intracellular concentrations of flavonoids in MDA-MB-231 cells using HPLC-coupled mass spectrometry / $c Anh Dang Pham.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2012
300 $a 1 electronic resource (68 pages)
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500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisors: White, Brandon Committee members: Okuda, Roy; d'Alarcao, Marc.
502 $b M.S. $c San Jose State University $d 2012.
520 $a Flavonoids are polyphenolic compounds found in a wide variety of plants. In recent years, flavonoids have been found to be beneficial to human health. Our lab has investigated the ability of flavonoids to induce cytotoxicity in human breast cancer cell lines. We have discovered that some compounds can induce cytotoxicity very readily, whereas others cannot. We hypothesized that this difference is attributable to a compound's ability to transverse the cell membrane. Therefore, we used LC-MS to measure the amount of each flavonoid that is able to enter into MDA-MB-231 cells, human breast cancer cells. We tested 14 flavonoids. MDA-MB-231 cells were plated and treated with 100 μM flavonoids for 24 and 48 hr. After treatment, cells were extracted using methanol. Flavonoids from extracts were characterized and quantitated by LC-MS. We found that intracellular flavonoid concentration increased over time for some of the flavonoids. We could not detect quercetin, myricetin, and catechin in cell extracts. However, quercetin was found to be a moderate inducer of cytotoxicity, whereas myricetin and catechin were not. We found that quercetin dimerized over time in the cell medium only in the presence of serum. The quercetin dimer could not be detected in cellular extracts, which suggested that it was unable to cross the membrane. Additionally, synthetic quercetin dimer did not induce cell death. Our results suggest that only fresh quercetin killed cells; once quercetin dimerized, it could not induce cell death.
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653 $a Mass spectrometry
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710 2 $a San Jose State University. $b Biological Sciences. $e degree granting institution. $3 3829232
720 1 $a White, Brandon $e degree supervisor.
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