東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Evaluation and Determination of the ...

Macharia, John Macharia,

Linked to FindBook

Google Book

Amazon

博客來

Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management = = Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management =/ John Macharia Macharia.
Reminder of title:	Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /
Author:	Macharia, John Macharia,
Description:	1 electronic resource (150 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
Contained By:	Dissertations Abstracts International86-03B.
Subject:	Fatalities. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31476312
ISBN:	9798384327233

Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management = = Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /
Macharia, John Macharia,

Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management =Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /John Macharia Macharia. - 1 electronic resource (150 pages)

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

Colorectal cancer is among the most common malignancies and the third prime cause of cancer-associated mortalities. Research into tumorigenic pathways can aid in the development of more efficient cancer therapies and provide insight into the physiological regulatory mechanisms employed by rapidly proliferating cancer cells. Due to the severe side effects of cancer chemotherapeutic medications, plant metabolites, and their analogs are now explored more frequently for the treatment and prevention of colorectal cancer (CRC), opening the stage for new phytotherapeutic strategies that are considered effective and safe substitutes. This extensive research aimed to evaluate and determine the phytotherapeutic properties of selected plants and their bioactive metabolites on targeted genes in colorectal cancer management. The plants evaluated were Withania somnifera L, Warbugia ugandensis, and Aloe secundiflora, while the targeted genes were COX-2, CASP9, Bcl-xL, Bcl2 and 5-LOX in CRC cell lines (Caco-2). To establish critical gaps in this important topic, extensive and well-thought systematic reviews were conducted at the initial stage of the study. Results obtained from the reviews informed the choice of target genes for selective consideration. Plant extracts were obtained using serial exhaustive extraction using three different extraction solvents with varying polarities, for maximum metabolites extraction (Hexane, Ethyl acetate, and Methanol). They were then dissolved in Dimethyl sulfoxide (DMSO) appropriately for bioassay. Caco-2 cell lines were passaged, and treated with plant extracts at varying concentrations, and their RNA's isolated for evaluation. Our unique study reports on these important plants as efficient natural inhibitors of CRC growth, by directly linking their phytoconstituents to; the downregulation of COX-2, 5-LOX, Bcl-xL, Bel2, and upregulation of CASP9 genes dose-dependently. I strongly present Withania somnifera, Warbugia ugandensis, and Aloe secundiflora plant extracts as promising natural inhibitors for CRC carcinogenesis and recommend in vivoand subsequent clinical trials, with substantial clinical effects postulated. In addition, methanol and ethyl acetate can extract metabolites exhibiting higher regulatory effects on the targeted genes and are thus highly recommended for application in yielding maximum benefits. Finally, I suggest studies on the identification and characterization of the specific metabolites in these plants, that are involved in the modulatory mechanisms, resulting in the inhibition of CRC growth and possible metastases.

English

ISBN: 9798384327233Subjects--Topical Terms:

3681792
Fatalities.

Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management = = Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /
LDR:04230nmm a22004213i 4500 001 2437687
005 20251124054957.5
006 m o d
007 cr|nu||||||||
008 261011s2023 miu||||||m |||||||eng d
020 $a 9798384327233
035 $a (MiAaPQD)AAI31476312
035 $a (MiAaPQD)Pecs44503
035 $a AAI31476312
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Macharia, John Macharia, $e author. $3 3828757
245 1 0 $a Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management = $b Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben / $c John Macharia Macharia.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 1 electronic resource (150 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
500 $a Advisors: Bence, L. Raposa; Zsolt, Kaposztas.
502 $b Ph.D. $c University of Pecs (Hungary) $d 2023.
520 $a Colorectal cancer is among the most common malignancies and the third prime cause of cancer-associated mortalities. Research into tumorigenic pathways can aid in the development of more efficient cancer therapies and provide insight into the physiological regulatory mechanisms employed by rapidly proliferating cancer cells. Due to the severe side effects of cancer chemotherapeutic medications, plant metabolites, and their analogs are now explored more frequently for the treatment and prevention of colorectal cancer (CRC), opening the stage for new phytotherapeutic strategies that are considered effective and safe substitutes. This extensive research aimed to evaluate and determine the phytotherapeutic properties of selected plants and their bioactive metabolites on targeted genes in colorectal cancer management. The plants evaluated were Withania somnifera L, Warbugia ugandensis, and Aloe secundiflora, while the targeted genes were COX-2, CASP9, Bcl-xL, Bcl2 and 5-LOX in CRC cell lines (Caco-2). To establish critical gaps in this important topic, extensive and well-thought systematic reviews were conducted at the initial stage of the study. Results obtained from the reviews informed the choice of target genes for selective consideration. Plant extracts were obtained using serial exhaustive extraction using three different extraction solvents with varying polarities, for maximum metabolites extraction (Hexane, Ethyl acetate, and Methanol). They were then dissolved in Dimethyl sulfoxide (DMSO) appropriately for bioassay. Caco-2 cell lines were passaged, and treated with plant extracts at varying concentrations, and their RNA's isolated for evaluation. Our unique study reports on these important plants as efficient natural inhibitors of CRC growth, by directly linking their phytoconstituents to; the downregulation of COX-2, 5-LOX, Bcl-xL, Bel2, and upregulation of CASP9 genes dose-dependently. I strongly present Withania somnifera, Warbugia ugandensis, and Aloe secundiflora plant extracts as promising natural inhibitors for CRC carcinogenesis and recommend in vivoand subsequent clinical trials, with substantial clinical effects postulated. In addition, methanol and ethyl acetate can extract metabolites exhibiting higher regulatory effects on the targeted genes and are thus highly recommended for application in yielding maximum benefits. Finally, I suggest studies on the identification and characterization of the specific metabolites in these plants, that are involved in the modulatory mechanisms, resulting in the inhibition of CRC growth and possible metastases.
546 $a English
590 $a School code: 2347
650 4 $a Fatalities. $3 3681792
650 4 $a Toxicity. $3 3564836
650 4 $a Flowers & plants. $3 3564028
650 4 $a Herbal medicine. $3 3564910
650 4 $a Phytochemicals. $3 915480
650 4 $a Mortality. $3 533218
650 4 $a Cancer therapies. $3 3557730
650 4 $a Alternative medicine. $3 536195
650 4 $a Botany. $3 516217
650 4 $a Oncology. $3 751006
650 4 $a Pharmaceutical sciences. $3 3173021
650 4 $a Toxicology. $3 556884
690 $a 0496
690 $a 0309
690 $a 0992
690 $a 0572
690 $a 0383
710 2 $a University of Pecs (Hungary). $b ETK Egeszsegtudomanyi Doktori Iskola. $e degree granting institution. $3 3828758
720 1 $a Bence, L. Raposa $e degree supervisor.
720 1 $a Zsolt, Kaposztas $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 86-03B.
790 $a 2347
791 $a Ph.D.
792 $a 2023
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31476312