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Neuroprotective Properties of Sangui...

Enn, Sam Si,

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Neuroprotective Properties of Sanguinoderma rugosum Extracts Against Glutamate-Induced Hippocampal Cells /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Neuroprotective Properties of Sanguinoderma rugosum Extracts Against Glutamate-Induced Hippocampal Cells // Sam Si Enn.
Author:	Enn, Sam Si,
Description:	1 electronic resource (96 pages)
Notes:	Source: Masters Abstracts International, Volume: 86-08.
Contained By:	Masters Abstracts International86-08.
Subject:	Amyotrophic lateral sclerosis. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31836453
ISBN:	9798304943963

Neuroprotective Properties of Sanguinoderma rugosum Extracts Against Glutamate-Induced Hippocampal Cells /
Enn, Sam Si,

Neuroprotective Properties of Sanguinoderma rugosum Extracts Against Glutamate-Induced Hippocampal Cells /Sam Si Enn. - 1 electronic resource (96 pages)

Source: Masters Abstracts International, Volume: 86-08.

Oxidative stress and neuroinflammation are two intertwined pathologic factors in a wide range of neurological diseases. With heightening prevalence of neurological diseases due to rising life expectancy around the globe, searching for novel and alternative therapeutic candidates is urgently needed. Sanguinoderma rugosum (synonym: Amauroderma rugosum) has been traditionally used as a natural remedy to reduce inflammation, prevent unceasing crying, cancer and epileptic fits in China and Malaysia. However, the potential neuroprotective and neurorescue effects of S. rugosum against glutamate-induced neurotoxicity remain largely unexplored. In the present study, extracts from S. rugosum were evaluated for their neuroprotective and neurorescue properties using in vitroHT-22 mouse hippocampal neuronal cells. The mycelia of S. rugosum were cultivated in submerged liquid culture and freeze-dried prior to solvent extraction. MTT cell viability assay was performed to determine the neurotoxicity, neuroprotective and neurorescue activities of S. rugosum extracts at 24 and 48 h. Flow cytometric analysis was conducted to investigate the effects of S. rugosum extracts on the intracellular reactive oxygen species (ROS) production and cell death induced by glutamate. The constituents present in SR-HF fraction were identified through GC/MS analysis. The preliminary findings showed that all extracts did not exhibit cytotoxic effect on HT-22 cells. Upon 24 h incubation, pre-treatment with both SR-EE (12.5 µg/mL) and SR-HF (100 µg/mL) markedly (P<0.05) restored the loss of cell viability to 65.80 ± 1.85% and 89.76 ± 9.50%, respectively. The glutamate-induced ROS generation was effectively declined by SR-EE and SR-HF to 13.8% and 8.0%, respectively. The population of late apoptotic/early Universiti Malaya necrotic cells showed reduction after pre-treatment with SR-HF, with value of 13.3%. However, an increase in the percentage of late apoptotic/early necrotic cells were shown in the glutamate-induced cells after pre-treatment with SR-EE, with value of 26.6%. In general, all extracts demonstrated neurorescue effect against glutamate-induced cells at 24 and 48 h. Eleven compounds were identified in SR-HF by GC/MS analysis, of which the linoleic acid, ergosterol and ethyl linoleate are the major chemical compounds. In short, these results demonstrated that SR-HF may be considered as a potent therapeutic agent to be used in treating neurological disorders related to oxidative stress and neuroinflammation.

English

ISBN: 9798304943963Subjects--Topical Terms:

1094680
Amyotrophic lateral sclerosis.
Subjects--Index Terms:

Medicinal mushroom

Neuroprotective Properties of Sanguinoderma rugosum Extracts Against Glutamate-Induced Hippocampal Cells /
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300 $a 1 electronic resource (96 pages)
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500 $a Advisors: Shin, Tan Yee; Shin, Sim Kae.
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520 $a Oxidative stress and neuroinflammation are two intertwined pathologic factors in a wide range of neurological diseases. With heightening prevalence of neurological diseases due to rising life expectancy around the globe, searching for novel and alternative therapeutic candidates is urgently needed. Sanguinoderma rugosum (synonym: Amauroderma rugosum) has been traditionally used as a natural remedy to reduce inflammation, prevent unceasing crying, cancer and epileptic fits in China and Malaysia. However, the potential neuroprotective and neurorescue effects of S. rugosum against glutamate-induced neurotoxicity remain largely unexplored. In the present study, extracts from S. rugosum were evaluated for their neuroprotective and neurorescue properties using in vitroHT-22 mouse hippocampal neuronal cells. The mycelia of S. rugosum were cultivated in submerged liquid culture and freeze-dried prior to solvent extraction. MTT cell viability assay was performed to determine the neurotoxicity, neuroprotective and neurorescue activities of S. rugosum extracts at 24 and 48 h. Flow cytometric analysis was conducted to investigate the effects of S. rugosum extracts on the intracellular reactive oxygen species (ROS) production and cell death induced by glutamate. The constituents present in SR-HF fraction were identified through GC/MS analysis. The preliminary findings showed that all extracts did not exhibit cytotoxic effect on HT-22 cells. Upon 24 h incubation, pre-treatment with both SR-EE (12.5 µg/mL) and SR-HF (100 µg/mL) markedly (P<0.05) restored the loss of cell viability to 65.80 ± 1.85% and 89.76 ± 9.50%, respectively. The glutamate-induced ROS generation was effectively declined by SR-EE and SR-HF to 13.8% and 8.0%, respectively. The population of late apoptotic/early Universiti Malaya necrotic cells showed reduction after pre-treatment with SR-HF, with value of 13.3%. However, an increase in the percentage of late apoptotic/early necrotic cells were shown in the glutamate-induced cells after pre-treatment with SR-EE, with value of 26.6%. In general, all extracts demonstrated neurorescue effect against glutamate-induced cells at 24 and 48 h. Eleven compounds were identified in SR-HF by GC/MS analysis, of which the linoleic acid, ergosterol and ethyl linoleate are the major chemical compounds. In short, these results demonstrated that SR-HF may be considered as a potent therapeutic agent to be used in treating neurological disorders related to oxidative stress and neuroinflammation.
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650 4 $a Mushrooms. $3 555435
650 4 $a Alzheimer's disease. $3 588010
650 4 $a Mitochondrial DNA. $3 558956
650 4 $a Metabolites. $3 683644
650 4 $a Cellular biology. $3 3172791
650 4 $a Neurosciences. $3 588700
653 $a Medicinal mushroom
653 $a Neurological disorder
653 $a Reactive oxygen species
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690 $a 0317
710 2 $a University of Malaya (Malaysia). $e degree granting institution. $3 3765814
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720 1 $a Shin, Sim Kae $e degree supervisor.
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790 $a 1293
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