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Adaptive strategies of Listeria mono...

Ma, Xuchuan,

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Adaptive strategies of Listeria monocytogenes: Evolved variants with altered stress resistance and fitness /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Adaptive strategies of Listeria monocytogenes: Evolved variants with altered stress resistance and fitness // Xuchuan Ma.
作者:	Ma, Xuchuan,
面頁冊數:	1 electronic resource (211 pages)
附註:	Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
Contained By:	Dissertations Abstracts International85-12B.
標題:	Listeria. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31472648
ISBN:	9798382828695

Adaptive strategies of Listeria monocytogenes: Evolved variants with altered stress resistance and fitness /
Ma, Xuchuan,

Adaptive strategies of Listeria monocytogenes: Evolved variants with altered stress resistance and fitness /Xuchuan Ma. - 1 electronic resource (211 pages)

Source: Dissertations Abstracts International, Volume: 85-12, Section: B.

To ensure the right to safe food, efficient strategies are required to control foodborne pathogens throughout the food supply chain. Among these pathogens, Listeria monocytogenes is particularly notable due to its low incidence but high case-fatality rates, ranging from 12 to 41 percent . This organism is ubiquitous, capable of being isolated from a wide range of environments including natural habitats, farms, silage, decaying vegetables, food production facilities, refrigerators, as well as human and animal feces. To adapt and survive the challenging conditions it encounters from soil to human hosts, L. monocytogenes employs a variety of protective strategies, one of which is population heterogeneity. Population heterogeneity, encompassing both genetic and non-genetic variability, generates phenotypic variation within a population, contributing to its fitness, adaptation, and survival. During the process of pathogen inactivation, variations in stress resistance among individual cells can cause deviations from expected linear inactivation patterns, resulting in a higher-than-expected number of surviving cells and selection of stress-resistant variants. Previously, 23 stable stress resistance L. monocytogenes variants have been isolated upon acid treatment of L. monocytogenes strain LO28, and 11 of the 23 variants had mutations in the rpsU gene locus, which encodes the ribosome 30S small sub-unit protein S21 (RpsU) These stress-resistant rpsU variants showed a trade-off between increased resistance and reduced growth rates. Further studies of the rpsU deletion variant V14 and the rpsU G50C variant V15 showed that both variants shared similar gene expression profiles and phenotypes compared to the WT. These findings suggest that rpsU deletion and the rpsU G50C mutation may impact the phenotype through a similar mechanism. Evolutionary experiments of variant V15 revealed that single amino acid substitutions in RpsU can facilitate a switch between states of high fitness and high stress resistance in L. monocytogenes, rising questions if variant V14 with complete rpsU deletion could also undergo a similar switching between multi-stress resistant and high fitness states.

English

ISBN: 9798382828695Subjects--Topical Terms:

3689378
Listeria.

Adaptive strategies of Listeria monocytogenes: Evolved variants with altered stress resistance and fitness /
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