東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

The Anti-Inflammatory Properties of ...

Todorov, Nikolay B.,

FindBook

Google Book

Amazon

博客來

The Anti-Inflammatory Properties of Exopolysaccharide From Bacillus subtilis /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The Anti-Inflammatory Properties of Exopolysaccharide From Bacillus subtilis // Nikolay B Todorov.
作者:	Todorov, Nikolay B.,
面頁冊數:	1 electronic resource (108 pages)
附註:	Source: Masters Abstracts International, Volume: 86-02.
Contained By:	Masters Abstracts International86-02.
標題:	Immunology. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31484553
ISBN:	9798383692202

The Anti-Inflammatory Properties of Exopolysaccharide From Bacillus subtilis /
Todorov, Nikolay B.,

The Anti-Inflammatory Properties of Exopolysaccharide From Bacillus subtilis /Nikolay B Todorov. - 1 electronic resource (108 pages)

Source: Masters Abstracts International, Volume: 86-02.

Commensal microorganisms can be used therapeutically to ameliorate a variety of diseases. The laboratory of Dr. Katherine L. Knight studies these therapeutic properties in the commensal microorganism known as Bacillus subtilis. Previously, the Knight lab has demonstrated that B. subtilis can protect against colitis caused by Citrobacter rodentium suggesting that B. subtilis possesses anti-inflammatory properties. These properties are specific to the polysaccharide capsule of the microorganism known as exopolysaccharide or EPS. EPS can be produced and purified for study. EPS has been demonstrated to be protective in a variety of diseases such as allergic eosinophilia, sepsis, Graft versus Host disease, and reduce proliferation of tumor cells in vitro.This dissertation focuses on the anti-inflammatory properties of EPS. It focuses on one aspect in vivo and another aspect in vitro. In the context of in vivo, LPS is a inflammatory polysaccharide that signals through TLR4. EPS also signals through TLR4 yet it induces an anti-inflammatory response in vivo. Therefore, the immune response of these molecules are very different and it became of interest to determine if EPS could induce an anti-inflammatory state against LPS known as endotoxin tolerance. Our results demonstrated that EPS could induce a state of endotoxin tolerance and it lasted for 1 week. Moreover, this inhibitory activity was mediated through innate cells,specifically, macrophages found in the small intestine. Endotoxin tolerance by EPS was specific to in vivo, but was not observed in vitro. Furthermore, we observed EPS downregulate cell surface expression of TLR4 in BMDCs in vitro suggesting that this is a potential mechanism by which EPS induces endotoxin tolerance.In addition to looking at the mechanism in the context of inflammation. It became also of interest to determine how EPS generates M2 anti-inflammatory macrophages. It has been previously demonstrated that EPS can generate M2 macrophages in vivo through intraperitoneal injection, but these macrophages cannot be generated in vitro through treating the cells directly with EPS. One potential explanation is that the environment in vitro is not suitable for the generation of M2 macrophages by EPS. We determined that transferred TLR4-KO peritoneal cells could become M2 macrophages upon EPS injection suggesting that the environment of the peritoneal cavity is required for their induction. We also observed the production of an M2 differentiating cytokine IL-4 in the serum suggesting that this is a potential mechanism by which M2 macrophages are being induced. Finally, we also demonstrated that transfer of peritoneal fluid or serum can induce M2 peritoneal macrophages in vitro.Taken collectively, data from this thesis demonstrate a novel mechanism that EPS works in vivo and it gives us mechanistic insights into how EPS generates anti-inflammatory M2 macrophages in vivo. This information regarding this polysaccharide will ideally allow a new therapy and can be utilized to treat diseases in the future.

English

ISBN: 9798383692202Subjects--Topical Terms:

611031
Immunology.
Subjects--Index Terms:

Microorganisms

The Anti-Inflammatory Properties of Exopolysaccharide From Bacillus subtilis /
LDR:04500nmm a22004333i 4500 001 2437084
005 20251119142635.5
006 m o d
007 cr|nu||||||||
008 261011s2024 miu||||||m |||||||eng d
020 $a 9798383692202
035 $a (MiAaPQD)AAI31484553
035 $a AAI31484553
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Todorov, Nikolay B., $e author. $3 3827964
245 1 0 $a The Anti-Inflammatory Properties of Exopolysaccharide From Bacillus subtilis / $c Nikolay B Todorov.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2024
300 $a 1 electronic resource (108 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 86-02.
500 $a Advisors: Knight, Katherine L. Committee members: Gallagher, Thomas; Sojka, Dorothy.
502 $b M.S. $c Loyola University Chicago $d 2024.
520 $a Commensal microorganisms can be used therapeutically to ameliorate a variety of diseases. The laboratory of Dr. Katherine L. Knight studies these therapeutic properties in the commensal microorganism known as Bacillus subtilis. Previously, the Knight lab has demonstrated that B. subtilis can protect against colitis caused by Citrobacter rodentium suggesting that B. subtilis possesses anti-inflammatory properties. These properties are specific to the polysaccharide capsule of the microorganism known as exopolysaccharide or EPS. EPS can be produced and purified for study. EPS has been demonstrated to be protective in a variety of diseases such as allergic eosinophilia, sepsis, Graft versus Host disease, and reduce proliferation of tumor cells in vitro.This dissertation focuses on the anti-inflammatory properties of EPS. It focuses on one aspect in vivo and another aspect in vitro. In the context of in vivo, LPS is a inflammatory polysaccharide that signals through TLR4. EPS also signals through TLR4 yet it induces an anti-inflammatory response in vivo. Therefore, the immune response of these molecules are very different and it became of interest to determine if EPS could induce an anti-inflammatory state against LPS known as endotoxin tolerance. Our results demonstrated that EPS could induce a state of endotoxin tolerance and it lasted for 1 week. Moreover, this inhibitory activity was mediated through innate cells,specifically, macrophages found in the small intestine. Endotoxin tolerance by EPS was specific to in vivo, but was not observed in vitro. Furthermore, we observed EPS downregulate cell surface expression of TLR4 in BMDCs in vitro suggesting that this is a potential mechanism by which EPS induces endotoxin tolerance.In addition to looking at the mechanism in the context of inflammation. It became also of interest to determine how EPS generates M2 anti-inflammatory macrophages. It has been previously demonstrated that EPS can generate M2 macrophages in vivo through intraperitoneal injection, but these macrophages cannot be generated in vitro through treating the cells directly with EPS. One potential explanation is that the environment in vitro is not suitable for the generation of M2 macrophages by EPS. We determined that transferred TLR4-KO peritoneal cells could become M2 macrophages upon EPS injection suggesting that the environment of the peritoneal cavity is required for their induction. We also observed the production of an M2 differentiating cytokine IL-4 in the serum suggesting that this is a potential mechanism by which M2 macrophages are being induced. Finally, we also demonstrated that transfer of peritoneal fluid or serum can induce M2 peritoneal macrophages in vitro.Taken collectively, data from this thesis demonstrate a novel mechanism that EPS works in vivo and it gives us mechanistic insights into how EPS generates anti-inflammatory M2 macrophages in vivo. This information regarding this polysaccharide will ideally allow a new therapy and can be utilized to treat diseases in the future.
546 $a English
590 $a School code: 0112
650 4 $a Immunology. $3 611031
650 4 $a Microbiology. $3 536250
650 4 $a Therapy. $3 3343697
653 $a Microorganisms
653 $a Bacillus subtilis
653 $a Exopolysaccharide
653 $a Anti-inflammatory properties
653 $a Endotoxin tolerance
690 $a 0982
690 $a 0212
690 $a 0410
710 2 $a Loyola University Chicago. $b Microbiology and Immunology. $e degree granting institution. $3 3827965
720 1 $a Knight, Katherine L. $e degree supervisor.
773 0 $t Masters Abstracts International $g 86-02.
790 $a 0112
791 $a M.S.
792 $a 2024
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31484553