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Effects of Dexmedetomidine on TLR4-M...

Hake, Whitney,

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Effects of Dexmedetomidine on TLR4-Mediated Innate Immune Response in Human Macrophages /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Effects of Dexmedetomidine on TLR4-Mediated Innate Immune Response in Human Macrophages // Whitney Hake, Kendra Davis.
Author:	Hake, Whitney,
other author:	Davis, Kendra ,
Description:	1 electronic resource (62 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
Contained By:	Dissertations Abstracts International86-03B.
Subject:	Immunology. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31146038
ISBN:	9798384100232

Effects of Dexmedetomidine on TLR4-Mediated Innate Immune Response in Human Macrophages /
Hake, Whitney,

Effects of Dexmedetomidine on TLR4-Mediated Innate Immune Response in Human Macrophages /Whitney Hake, Kendra Davis. - 1 electronic resource (62 pages)

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

Background: The United States population greater than 65 years old is rapidly growing, further increasing the number of surgeries that inherently come with age. Postoperative cognitive dysfunction (POCD), caused by neuroinflammation, is a decline in cognitive function after surgery that does not depend on type of surgery or anesthetic, and the only identifiable independent risk factor is advanced age. Dexmedetomidine has been shown to decrease the incidence of neuroinflammation via attenuation of TLR4 and its related proteins in murine models, and the incidence of POCD in human meta-analyses. The purpose of this study was to evaluate the effect dexmedetomidine has on TLR4 and TLR4-related proteins after stress with LPS in human macrophages. Methodology: This study was an experimental in vitro analysis of TLR4 and TLR4-related protein expression after stress with LPS and treatment with dexmedetomidine. HMC-3 and KG-1 macrophages were cultured under standard conditions and stressed with LPS for 24 hours. After 24 hours, the cells were treated with varying doses of dexmedetomidine. After 3 hours of treatment, the samples were processed and expression of TLR4 and TLR4-related proteins were measured. Results: One way ANOVA was used to compare replicates averaged together against the control. Tukey's test was used for individual comparisons between groups. There was no significance demonstrated in the expression of TLR4 and its related proteins between the LPS group and any dexmedetomidine group. Conclusions: The results of this study suggest that dexmedetomidine does not affect TLR4-related functions of human macrophages.

English

ISBN: 9798384100232Subjects--Topical Terms:

611031
Immunology.
Subjects--Index Terms:

Dexmedetomidine

Effects of Dexmedetomidine on TLR4-Mediated Innate Immune Response in Human Macrophages /
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520 $a Background: The United States population greater than 65 years old is rapidly growing, further increasing the number of surgeries that inherently come with age. Postoperative cognitive dysfunction (POCD), caused by neuroinflammation, is a decline in cognitive function after surgery that does not depend on type of surgery or anesthetic, and the only identifiable independent risk factor is advanced age. Dexmedetomidine has been shown to decrease the incidence of neuroinflammation via attenuation of TLR4 and its related proteins in murine models, and the incidence of POCD in human meta-analyses. The purpose of this study was to evaluate the effect dexmedetomidine has on TLR4 and TLR4-related proteins after stress with LPS in human macrophages. Methodology: This study was an experimental in vitro analysis of TLR4 and TLR4-related protein expression after stress with LPS and treatment with dexmedetomidine. HMC-3 and KG-1 macrophages were cultured under standard conditions and stressed with LPS for 24 hours. After 24 hours, the cells were treated with varying doses of dexmedetomidine. After 3 hours of treatment, the samples were processed and expression of TLR4 and TLR4-related proteins were measured. Results: One way ANOVA was used to compare replicates averaged together against the control. Tukey's test was used for individual comparisons between groups. There was no significance demonstrated in the expression of TLR4 and its related proteins between the LPS group and any dexmedetomidine group. Conclusions: The results of this study suggest that dexmedetomidine does not affect TLR4-related functions of human macrophages.
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