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Integrated Approaches to Understand ...

Choi, Naeyeoung,

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Integrated Approaches to Understand Both Effector-Triggered and Broad-Spectrum Resistance in Rice Against Magnaporthe oryzae /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Integrated Approaches to Understand Both Effector-Triggered and Broad-Spectrum Resistance in Rice Against Magnaporthe oryzae // Naeyeoung Choi.
Author:	Choi, Naeyeoung,
Description:	1 electronic resource (264 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
Contained By:	Dissertations Abstracts International86-12B.
Subject:	Biochemistry. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=32111846
ISBN:	9798315737308

Integrated Approaches to Understand Both Effector-Triggered and Broad-Spectrum Resistance in Rice Against Magnaporthe oryzae /
Choi, Naeyeoung,

Integrated Approaches to Understand Both Effector-Triggered and Broad-Spectrum Resistance in Rice Against Magnaporthe oryzae /Naeyeoung Choi. - 1 electronic resource (264 pages)

Source: Dissertations Abstracts International, Volume: 86-12, Section: B.

Rice (Oryza sativa L.), a staple crop for nearly 50% of the global population, particularly in Asia, is highly susceptible to various pathogens, leading to significant yield losses. To understand the molecular mechanisms underlying rice defense, two projects were carried out in this dissertation. The first study (Chapter 2) utilized rice as a model to examine transcriptional and metabolomic changes associated with the resistance (R) gene, Piz-t. Transcriptomic analyses revealed 2,791 Piz-t-specific differentially expressed genes (DEGs) following inoculation with Magnaporthe oryzae strain RO1-1. These findings highlighted correlations between gene expression and metabolite alterations, including changes in terpenoids, phenylpropanoids, flavonoids, fatty acids, amino acids, glycolysis/TCA intermediates, and phenylalanine pathways, providing critical insights into how R gene-mediated resistance enhances disease resistance in rice.The focus of the second study (Chapter 3) is to understand the role of programmed cell death (PCD) in rice immunity. Rice SPL11 cell-death suppressor 2 (SDS2), a receptor-like protein kinase, regulates PCD by phosphorylating and interacting with the U-box E3 ligase SPL11, as negative regulator of cell death, OsRLCK118 and OsRLCK176. The phosphorylated OsRLCK118 interacts with OsRbohB to mediate reactive oxygen species (ROS) generation. Despite these insights, the downstream pathways of SDS2-mediated resistance remain unclear. RNA-seq analysis of SDS2-ACT indicated higher expression of OsBDG1, suggesting possible interactor of SDS2, localizing to both the non-nuclear and nuclear area. OsBDG1 is a small protein with an LRR N-terminal domain and three LRR domains at its C-terminus, regulating in leaf inclination and grain size but no study was reported in disease resistance. Reduced luciferase and GUS activity were found by the co-expression of 35s::SDS2-HA and 35s::OsBDG1-GFP in rice protoplasts relative to the co-expression of 35s:HA and 35s:GFP as control, suggesting enhanced PCD. OsBDG1 also interacted with OsRLCK118, OsRLCK176, and OsRbohB, in close relation with up-regulation of chitinase, ROS accumulation, and cell death responses. Overexpression of OsBDG1 enhanced resistance to M. oryzae, while the E3 ligase OsRING27 mediated SDS2-dependent OsBDG1 degradation. This might cause overexpression of OsRING27 enhanced susceptibility but increased resistance against M. oryzae. This dissertation provides novel insights into Piz-t- and SDS2-mediated resistance mechanisms, revealing the intricate molecular dynamics of gene regulation, metabolite synthesis, and PCD in rice immunity.

English

ISBN: 9798315737308Subjects--Topical Terms:

518028
Biochemistry.
Subjects--Index Terms:

Magnaporthe oryzae

Integrated Approaches to Understand Both Effector-Triggered and Broad-Spectrum Resistance in Rice Against Magnaporthe oryzae /
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520 $a Rice (Oryza sativa L.), a staple crop for nearly 50% of the global population, particularly in Asia, is highly susceptible to various pathogens, leading to significant yield losses. To understand the molecular mechanisms underlying rice defense, two projects were carried out in this dissertation. The first study (Chapter 2) utilized rice as a model to examine transcriptional and metabolomic changes associated with the resistance (R) gene, Piz-t. Transcriptomic analyses revealed 2,791 Piz-t-specific differentially expressed genes (DEGs) following inoculation with Magnaporthe oryzae strain RO1-1. These findings highlighted correlations between gene expression and metabolite alterations, including changes in terpenoids, phenylpropanoids, flavonoids, fatty acids, amino acids, glycolysis/TCA intermediates, and phenylalanine pathways, providing critical insights into how R gene-mediated resistance enhances disease resistance in rice.The focus of the second study (Chapter 3) is to understand the role of programmed cell death (PCD) in rice immunity. Rice SPL11 cell-death suppressor 2 (SDS2), a receptor-like protein kinase, regulates PCD by phosphorylating and interacting with the U-box E3 ligase SPL11, as negative regulator of cell death, OsRLCK118 and OsRLCK176. The phosphorylated OsRLCK118 interacts with OsRbohB to mediate reactive oxygen species (ROS) generation. Despite these insights, the downstream pathways of SDS2-mediated resistance remain unclear. RNA-seq analysis of SDS2-ACT indicated higher expression of OsBDG1, suggesting possible interactor of SDS2, localizing to both the non-nuclear and nuclear area. OsBDG1 is a small protein with an LRR N-terminal domain and three LRR domains at its C-terminus, regulating in leaf inclination and grain size but no study was reported in disease resistance. Reduced luciferase and GUS activity were found by the co-expression of 35s::SDS2-HA and 35s::OsBDG1-GFP in rice protoplasts relative to the co-expression of 35s:HA and 35s:GFP as control, suggesting enhanced PCD. OsBDG1 also interacted with OsRLCK118, OsRLCK176, and OsRbohB, in close relation with up-regulation of chitinase, ROS accumulation, and cell death responses. Overexpression of OsBDG1 enhanced resistance to M. oryzae, while the E3 ligase OsRING27 mediated SDS2-dependent OsBDG1 degradation. This might cause overexpression of OsRING27 enhanced susceptibility but increased resistance against M. oryzae. This dissertation provides novel insights into Piz-t- and SDS2-mediated resistance mechanisms, revealing the intricate molecular dynamics of gene regulation, metabolite synthesis, and PCD in rice immunity.
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653 $a Metabolomics
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