東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Functional Diversification of Rice A...

Jouni, Rachel,

Linked to FindBook

Google Book

Amazon

博客來

Functional Diversification of Rice AGO1 Proteins /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Functional Diversification of Rice AGO1 Proteins // Rachel Jouni.
Author:	Jouni, Rachel,
Description:	1 electronic resource (175 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-11, Section: B.
Contained By:	Dissertations Abstracts International86-11B.
Subject:	Plant sciences. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=32002919
ISBN:	9798314854068

Functional Diversification of Rice AGO1 Proteins /
Jouni, Rachel,

Functional Diversification of Rice AGO1 Proteins /Rachel Jouni. - 1 electronic resource (175 pages)

Source: Dissertations Abstracts International, Volume: 86-11, Section: B.

Global demand for food is outpacing gains from conventional plant breeding. Scientists have turned to molecular crop breeding techniques to increase crop output and mitigate global hunger; however, a common setback in this process is the pleiotropic effects of target genes. We need to develop strategies to specifically modulate desired traits without incurring undesired penalties. Natural evolution frequently balances such tradeoffs through extensive regulation, which we can leverage to produce more specific results. Reproductive development is an important target of breeding efforts due to its direct effect on yield. Rice, a major staple food crop for half the world's population, is a model organism due to its well-annotated genome and genetic tractability, making it an ideal candidate for molecular study. Small RNAs (sRNAs) are an important and widespread mechanism of regulation in plants, and sRNA pathways have expanded over time. To study the sRNA landscape during anther development of rice, we created sRNA and expression atlases of anther development in Kitaake rice and discovered new compositionally and temporally distinct clusters of 21- and 24-nt phasiRNAs. Though there are thousands of sRNAs, there are only 19 Argonaute (AGO) proteins in rice, which load sRNAs and carry out gene repression, making them attractive candidates for breeding efforts. We studied the functional diversification of the four rice AGO1 proteins, since AtAgo1 is known to play a role in plant development and stress response, thus making AGO1 a good candidate for study regarding regulation for specificity. We studied different potential mechanisms of differentiation and found evidence of neofunctionalization based on spatiotemporal expression and subcellular localization. Furthermore, we found an erect panicle mutant phenotype of the osago1b single mutant. These findings provide us with potential molecular mechanisms driving specific functions among paralogs, which we can leverage to uncouple plieotropic effects in crop breeding efforts. 

English

ISBN: 9798314854068Subjects--Topical Terms:

3173832
Plant sciences.
Subjects--Index Terms:

Anther development

Functional Diversification of Rice AGO1 Proteins /
LDR:03579nmm a22004693i 4500 001 2435850
005 20251029060406.5
006 m o d
007 cr|nu||||||||
008 261011s2025 miu||||||m |||||||eng d
020 $a 9798314854068
035 $a (MiAaPQD)AAI32002919
035 $a AAI32002919
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Jouni, Rachel, $e author. $0 (orcid)0000-0002-7456-1762 $3 3826310
245 1 0 $a Functional Diversification of Rice AGO1 Proteins / $c Rachel Jouni.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2025
300 $a 1 electronic resource (175 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 86-11, Section: B.
500 $a Advisors: Meyers, Blake Committee members: Baldrich, Patricia; Carrington, James; Kellogg, Elizabeth; Kunkel, Barbara; Nusinow, Dmitri.
502 $b Ph.D. $c Washington University in St. Louis $d 2025.
520 $a Global demand for food is outpacing gains from conventional plant breeding. Scientists have turned to molecular crop breeding techniques to increase crop output and mitigate global hunger; however, a common setback in this process is the pleiotropic effects of target genes. We need to develop strategies to specifically modulate desired traits without incurring undesired penalties. Natural evolution frequently balances such tradeoffs through extensive regulation, which we can leverage to produce more specific results. Reproductive development is an important target of breeding efforts due to its direct effect on yield. Rice, a major staple food crop for half the world's population, is a model organism due to its well-annotated genome and genetic tractability, making it an ideal candidate for molecular study. Small RNAs (sRNAs) are an important and widespread mechanism of regulation in plants, and sRNA pathways have expanded over time. To study the sRNA landscape during anther development of rice, we created sRNA and expression atlases of anther development in Kitaake rice and discovered new compositionally and temporally distinct clusters of 21- and 24-nt phasiRNAs. Though there are thousands of sRNAs, there are only 19 Argonaute (AGO) proteins in rice, which load sRNAs and carry out gene repression, making them attractive candidates for breeding efforts. We studied the functional diversification of the four rice AGO1 proteins, since AtAgo1 is known to play a role in plant development and stress response, thus making AGO1 a good candidate for study regarding regulation for specificity. We studied different potential mechanisms of differentiation and found evidence of neofunctionalization based on spatiotemporal expression and subcellular localization. Furthermore, we found an erect panicle mutant phenotype of the osago1b single mutant. These findings provide us with potential molecular mechanisms driving specific functions among paralogs, which we can leverage to uncouple plieotropic effects in crop breeding efforts. 
546 $a English
590 $a School code: 0252
650 4 $a Plant sciences. $3 3173832
650 4 $a Molecular biology. $3 517296
650 4 $a Physiology. $3 518431
650 4 $a Biology. $3 522710
650 4 $a Genetics. $3 530508
653 $a Anther development
653 $a Argonaute
653 $a Genetic regulation
653 $a Genomics
653 $a PhasiRNAs
653 $a Small RNAs
690 $a 0479
690 $a 0307
690 $a 0306
690 $a 0369
690 $a 0719
710 2 $a Washington University in St. Louis. $b Biology & Biomedical Sciences (Plant & Microbial Biosciences). $e degree granting institution. $3 3826311
720 1 $a Meyers, Blake $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 86-11B.
790 $a 0252
791 $a Ph.D.
792 $a 2025
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=32002919