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Modeling the Bovine Intestine: Integ...

Dykstra, Gerald D.,

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Modeling the Bovine Intestine: Integrating 3D Organoids and 2D Monolayers to Advance Gut Health and Pathogen Research /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Modeling the Bovine Intestine: Integrating 3D Organoids and 2D Monolayers to Advance Gut Health and Pathogen Research // Gerald D Dykstra.
Author:	Dykstra, Gerald D.,
Description:	1 electronic resource (140 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
Contained By:	Dissertations Abstracts International86-12B.
Subject:	Animal sciences. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31762304
ISBN:	9798286439492

Modeling the Bovine Intestine: Integrating 3D Organoids and 2D Monolayers to Advance Gut Health and Pathogen Research /
Dykstra, Gerald D.,

Modeling the Bovine Intestine: Integrating 3D Organoids and 2D Monolayers to Advance Gut Health and Pathogen Research /Gerald D Dykstra. - 1 electronic resource (140 pages)

Source: Dissertations Abstracts International, Volume: 86-12, Section: B.

Advancements in bovine intestinal organoid technology have unlocked new potential for modeling intestinal physiology and pathology in vitro. This dissertation explores the establishment, characterization, and application of neonatal bovine intestinal organoids derived from various gut segments, including the duodenum, jejunum, ileum, colon, and rectum, utilizing novel sampling and culture methods. The generated organoids faithfully replicate the multicellular architecture and functional dynamics of the bovine intestine, exhibiting cellular polarity, diverse epithelial lineages, and robust self-renewal, making them a physiologically relevant model for intestinal research.The dissertation presents protocols for developing ileal and rectal organoid-derived monolayers from adult cattle, addressing challenges in luminal accessibility critical for host-pathogen interaction studies. Although rectal organoid-derived monolayers were successfully generated from neonatal bovine intestinal organoids, ileal monolayers were not, emphasizing age-specific variability in organoid technology. These monolayers demonstrated functional barrier integrity and cellular diversity, supporting their utility in advanced in vitro studies.This research confirms the ability to generate neonatal bovine intestinal organoids and organoid-derived monolayers, establishing a foundational framework for applying bovine intestinal organoid models in veterinary medicine, agricultural science, and zoonotic disease research. By bridging the gap between traditional cell culture and animal models, these findings advance gut health research in species critical to both veterinary and human health.

English

ISBN: 9798286439492Subjects--Topical Terms:

3174829
Animal sciences.
Subjects--Index Terms:

Bovine

Modeling the Bovine Intestine: Integrating 3D Organoids and 2D Monolayers to Advance Gut Health and Pathogen Research /
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245 1 0 $a Modeling the Bovine Intestine: Integrating 3D Organoids and 2D Monolayers to Advance Gut Health and Pathogen Research / $c Gerald D Dykstra.
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520 $a Advancements in bovine intestinal organoid technology have unlocked new potential for modeling intestinal physiology and pathology in vitro. This dissertation explores the establishment, characterization, and application of neonatal bovine intestinal organoids derived from various gut segments, including the duodenum, jejunum, ileum, colon, and rectum, utilizing novel sampling and culture methods. The generated organoids faithfully replicate the multicellular architecture and functional dynamics of the bovine intestine, exhibiting cellular polarity, diverse epithelial lineages, and robust self-renewal, making them a physiologically relevant model for intestinal research.The dissertation presents protocols for developing ileal and rectal organoid-derived monolayers from adult cattle, addressing challenges in luminal accessibility critical for host-pathogen interaction studies. Although rectal organoid-derived monolayers were successfully generated from neonatal bovine intestinal organoids, ileal monolayers were not, emphasizing age-specific variability in organoid technology. These monolayers demonstrated functional barrier integrity and cellular diversity, supporting their utility in advanced in vitro studies.This research confirms the ability to generate neonatal bovine intestinal organoids and organoid-derived monolayers, establishing a foundational framework for applying bovine intestinal organoid models in veterinary medicine, agricultural science, and zoonotic disease research. By bridging the gap between traditional cell culture and animal models, these findings advance gut health research in species critical to both veterinary and human health.
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653 $a Organoid-derived monolayers
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710 2 $a Washington State University. $b College of Veterinary Medicine. $e degree granting institution. $3 3815813
720 1 $a Burbick, Claire R. $e degree supervisor.
720 1 $a McConnel, Craig S. $e degree supervisor.
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791 $a Ph.D.
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