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Evaluation of Enumeration Methods fo...

Dhatt, Ujjalpreet Kaur,

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Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry // Ujjalpreet Kaur Dhatt.
Author:	Dhatt, Ujjalpreet Kaur,
Description:	1 electronic resource (106 pages)
Notes:	Source: Masters Abstracts International, Volume: 86-07.
Contained By:	Masters Abstracts International86-07.
Subject:	Food science. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31632903
ISBN:	9798346894438

Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry /
Dhatt, Ujjalpreet Kaur,

Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry /Ujjalpreet Kaur Dhatt. - 1 electronic resource (106 pages)

Source: Masters Abstracts International, Volume: 86-07.

Controlling Salmonella and Campylobacter in poultry requires robust quantification methods. This study compares enumeration methods in ground turkey, ground chicken, and chicken wings at varied inoculum levels (~8 log CFU/mL for high and ~5 log CFU/mL for low-level inoculation). Salmonella was quantified using conventional plating, miniaturized most probable number, and two PCR-based quantification assays. For Campylobacter, conventional plating, automated MPN, and PCR-based quantification assays were tested. ANOVA and Tukey's test revealed at high inoculation levels for Salmonella, mMPN resulted in the highest levels (p ≤ 0.05), followed by PCR assay 1, plating, and PCR assay 2. At low levels, mMPN, PCR Assay 1 & 2 performed similarly (p > 0.05) with plating significantly lower. For Campylobacter, the plating was higher than automated MPN at high levels (p ≤ 0.05), but PCR assay was significantly higher than both plating and automated MPN (p <.0001) at low levels. Traditional methods are reliable at higher contamination levels, whereas PCR-based precision is at low contamination levels.

English

ISBN: 9798346894438Subjects--Topical Terms:

3173303
Food science.
Subjects--Index Terms:

Campylobacter

Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry /
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245 1 0 $a Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry / $c Ujjalpreet Kaur Dhatt.
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300 $a 1 electronic resource (106 pages)
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500 $a Source: Masters Abstracts International, Volume: 86-07.
500 $a Advisors: Singh, Manpreet Committee members: Mishra, Abhinav; Critzer, Faith.
502 $b M.S. $c University of Georgia $d 2024.
520 $a Controlling Salmonella and Campylobacter in poultry requires robust quantification methods. This study compares enumeration methods in ground turkey, ground chicken, and chicken wings at varied inoculum levels (~8 log CFU/mL for high and ~5 log CFU/mL for low-level inoculation). Salmonella was quantified using conventional plating, miniaturized most probable number, and two PCR-based quantification assays. For Campylobacter, conventional plating, automated MPN, and PCR-based quantification assays were tested. ANOVA and Tukey's test revealed at high inoculation levels for Salmonella, mMPN resulted in the highest levels (p ≤ 0.05), followed by PCR assay 1, plating, and PCR assay 2. At low levels, mMPN, PCR Assay 1 & 2 performed similarly (p > 0.05) with plating significantly lower. For Campylobacter, the plating was higher than automated MPN at high levels (p ≤ 0.05), but PCR assay was significantly higher than both plating and automated MPN (p <.0001) at low levels. Traditional methods are reliable at higher contamination levels, whereas PCR-based precision is at low contamination levels.
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590 $a School code: 0077
650 4 $a Food science. $3 3173303
650 4 $a Microbiology. $3 536250
650 4 $a Public health. $3 534748
650 4 $a Animal sciences. $3 3174829
653 $a Campylobacter
653 $a Enumeration
653 $a Most probable number
653 $a Prevalence
653 $a Salmonella
653 $a Quantification methods
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690 $a 0475
690 $a 0410
690 $a 0573
710 2 $a University of Georgia. $b Food Science - MS. $e degree granting institution. $3 3826002
720 1 $a Singh, Manpreet $e degree supervisor.
773 0 $t Masters Abstracts International $g 86-07.
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791 $a M.S.
792 $a 2024
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