東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Correlation between a system for ami...

Qian, Nan-Xin,

FindBook

Google Book

Amazon

博客來

Correlation between a system for amino acid transport and sodium, potassium activated adenosine triphosphatase in Chinese hamster ovary cells /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Correlation between a system for amino acid transport and sodium, potassium activated adenosine triphosphatase in Chinese hamster ovary cells // Nan-Xin Qian.
作者:	Qian, Nan-Xin,
面頁冊數:	1 electronic resource (131 pages)
附註:	Source: Dissertations Abstracts International, Volume: 53-10, Section: B.
Contained By:	Dissertations Abstracts International53-10B.
標題:	Genetics. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9208365
ISBN:	9798207237497

Correlation between a system for amino acid transport and sodium, potassium activated adenosine triphosphatase in Chinese hamster ovary cells /
Qian, Nan-Xin,

Correlation between a system for amino acid transport and sodium, potassium activated adenosine triphosphatase in Chinese hamster ovary cells /Nan-Xin Qian. - 1 electronic resource (131 pages)

Source: Dissertations Abstracts International, Volume: 53-10, Section: B.

Mutants for the A system of amino acid transport, ala$\\sp{\\rm r}$4, H2.1, H3.9, and H3.9r, have increased activity and abundance for the A system transporter, increased sensitivity to ouabain, and increased activity and abundance of the Na$\\sp{+}$,K$\\sp{+}$-ATPase compared to their parental wild type (WT) Chinese hamster ovary cells (CHO-K1). This increase in the Na$\\sp{+}$,K$\\sp{+}$-ATPase activity and abundance is accompanied by an increase in alpha 1 subunit mRNA of this enzyme. No mRNA for the beta subunit was detectable. These increases in activity, abundance, and alpha 1 mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase are closely correlated with the increased A system V$\\sb{\\rm max}$ in these cells, suggesting the co-regulation of these two closely related systems. Evidence is presented that the increase in Na$\\sp{+}$,K$\\sp{+}$-ATPase activity as exhibited in A system mutant ala$\\sp{\\rm r}$4 is due to a co-regulation between the A system and the Na$\\sp{+}$,K$\\sp{+}$-ATPase by regulatory gene R1. Mutant ala$\\sp{\\rm r}$4 is constitutive for A system and exhibits an increase in Na$\\sp{+}$,K$\\sp{+}$-ATPase activity and abundance, and an increase in the mRNA for the alpha 1 subunit. Amino acid starvation which derepresses A system activity also results in an increase in abundance of the alpha 1 mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase in WT cells. Co-repressors for A system prevent increases in A system activity and the alpha 1 mRNA for the pump to the same extent, while non-co-repressors fail to prevent these increases. Hybrids of ala$\\sp{\\rm r}$4 x WT have WT phenotype for both A system activity and the Na$\\sp{+}$,K$\\sp{+}$-ATPase alpha 1 mRNA level. The increases in alpha 1 mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase are not due to gene amplification or gene demethylation in ala$\\sp{\\rm r}$4 or in H2.1, H3.9, and H3.9r. There is no evidence that other isoforms of this enzyme are induced in these mutants or that another regulatory gene R2 for A system is involved in the increase in alpha 1 mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase. Besides the regulatory gene R1, other possible genes that co-regulate the A system and the alpha 1 subunit mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase for H2.1, H3.9, and H3.9r are also discussed.

English

ISBN: 9798207237497Subjects--Topical Terms:

530508
Genetics.

Correlation between a system for amino acid transport and sodium, potassium activated adenosine triphosphatase in Chinese hamster ovary cells /
LDR:03607nmm a22003733i 4500 001 2435365
005 20251001074921.5
006 m o d
007 cr|nu||||||||
008 261011s1991 miu||||||m |||||||eng d
020 $a 9798207237497
035 $a (MiAaPQD)AAI9208365
035 $a AAI9208365
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Qian, Nan-Xin, $e author. $3 3825656
245 1 0 $a Correlation between a system for amino acid transport and sodium, potassium activated adenosine triphosphatase in Chinese hamster ovary cells / $c Nan-Xin Qian.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 1991
300 $a 1 electronic resource (131 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 53-10, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisors: Englesberg, Ellis.
502 $b Ph.D. $c University of California, Santa Barbara $d 1991.
520 $a Mutants for the A system of amino acid transport, ala$\\sp{\\rm r}$4, H2.1, H3.9, and H3.9r, have increased activity and abundance for the A system transporter, increased sensitivity to ouabain, and increased activity and abundance of the Na$\\sp{+}$,K$\\sp{+}$-ATPase compared to their parental wild type (WT) Chinese hamster ovary cells (CHO-K1). This increase in the Na$\\sp{+}$,K$\\sp{+}$-ATPase activity and abundance is accompanied by an increase in alpha 1 subunit mRNA of this enzyme. No mRNA for the beta subunit was detectable. These increases in activity, abundance, and alpha 1 mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase are closely correlated with the increased A system V$\\sb{\\rm max}$ in these cells, suggesting the co-regulation of these two closely related systems. Evidence is presented that the increase in Na$\\sp{+}$,K$\\sp{+}$-ATPase activity as exhibited in A system mutant ala$\\sp{\\rm r}$4 is due to a co-regulation between the A system and the Na$\\sp{+}$,K$\\sp{+}$-ATPase by regulatory gene R1. Mutant ala$\\sp{\\rm r}$4 is constitutive for A system and exhibits an increase in Na$\\sp{+}$,K$\\sp{+}$-ATPase activity and abundance, and an increase in the mRNA for the alpha 1 subunit. Amino acid starvation which derepresses A system activity also results in an increase in abundance of the alpha 1 mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase in WT cells. Co-repressors for A system prevent increases in A system activity and the alpha 1 mRNA for the pump to the same extent, while non-co-repressors fail to prevent these increases. Hybrids of ala$\\sp{\\rm r}$4 x WT have WT phenotype for both A system activity and the Na$\\sp{+}$,K$\\sp{+}$-ATPase alpha 1 mRNA level. The increases in alpha 1 mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase are not due to gene amplification or gene demethylation in ala$\\sp{\\rm r}$4 or in H2.1, H3.9, and H3.9r. There is no evidence that other isoforms of this enzyme are induced in these mutants or that another regulatory gene R2 for A system is involved in the increase in alpha 1 mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase. Besides the regulatory gene R1, other possible genes that co-regulate the A system and the alpha 1 subunit mRNA of the Na$\\sp{+}$,K$\\sp{+}$-ATPase for H2.1, H3.9, and H3.9r are also discussed.
546 $a English
590 $a School code: 0035
650 4 $a Genetics. $3 530508
650 4 $a Psychobiology. $3 555678
690 $a 0369
690 $a 0349
710 2 $a University of California, Santa Barbara. $e degree granting institution. $3 3818149
720 1 $a Englesberg, Ellis $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 53-10B.
790 $a 0035
791 $a Ph.D.
792 $a 1991
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9208365