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Evaluation of the Prebiotic Potential of Coriolus Versicolor : Effect upon the Human Gut Microbiota /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Evaluation of the Prebiotic Potential of Coriolus Versicolor : Effect upon the Human Gut Microbiota // Celia Maria da Silva Freitas Costa.
Author:	Costa, Celia Maria da Silva Freitas,
Description:	1 electronic resource (102 pages)
Notes:	Source: Masters Abstracts International, Volume: 85-08.
Contained By:	Masters Abstracts International85-08.
Subject:	Pathogens. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30829624
ISBN:	9798381541779

Evaluation of the Prebiotic Potential of Coriolus Versicolor : Effect upon the Human Gut Microbiota /
Costa, Celia Maria da Silva Freitas,

Evaluation of the Prebiotic Potential of Coriolus Versicolor : Effect upon the Human Gut Microbiota /Celia Maria da Silva Freitas Costa. - 1 electronic resource (102 pages)

Source: Masters Abstracts International, Volume: 85-08.

The human gut microbiota has become increasingly relevant when considering human health and disease due to its connection with the immune system and human metabolism. One way of actively modulating the gut microbiota is through the ingestion of prebiotics, that can be fermented by beneficial members of the colonic population, resulting in the production of certain metabolites regarded as beneficial, such as Short Chain Fatty Acids (SCFA). Coriolus versicolor is a mushroom that, while known for its immunomodulatory and anticarcinogenic properties has also been previously described as possessing some prebiotic potential. However, most studies focus on the potentialities of C. versicolor extracts, leaving the properties of its biomass unstudied. Hence, the main goal of the present work was to evaluate the prebiotic potential in a commercial supplement of C. versicolorbiomass using an in vitro faecal model. In order to achieve the proposed goal, the biomass was first submitted to a digestion simulation after which, it was used to perform human faecal fermentations using faeces from five distinct donors. Samples were collected at 0, 12, 24 and 48 h, for pH measurements and to be characterized regarding organic acid production, with emphasis on SCFA (through HPLC), as well as characterize the microbial profile (through qPCR).Despite the intrinsic variability of the human gut microbiota between individuals, the presence of C. versicolor biomass consistently resulted in an increase in the number of Bifidobacterium's 16S rRNA gene copies, for all donors when comparing to the negative control, although the overall increase was lower than the one registered for the positive control (i.e. FOS). This behaviour was observed after 24 h (negative control- 0.203 ± 0.017; positive control- 0.282 ± 0.011; C. versicolor- 0.238 ± 0.008, expressed in log of copy numbers of 16S rRNA per ng of DNA) and 48 h (negative control- 0.212 ± 0.016; positive control- 0.301 ± 0.027; C. versicolor- 0.268 ± 0.011, expressed in log of copy numbers of 16S rRNA per ng of DNA), but no significant differences were found between the assayed conditions after 12 h incubation. For the remaining bacterial groups characterized (Firmicutes, Bacteroidetes, Bacteroides, Clostridium leptum and Lactobacillus) it was not possible to observe a general trend but only donor specific behaviours. Overall, both C. versicolor and FOS resulted in a decrease of the pH values, in opposition to the negative control, which confirmed the production of acids as a result of bacterial metabolism. As for SCFA and lactic acid quantification, at the end of the fermentation, C. versicolor biomass led to the production of acetic acid (0.212 ± 0.088 mg mL-1), lactic acid (0.032 ± 0.002 mg mL-1) and propionic acid (0.0184 ± 0.007 mg mL-1). In sum, it is possible to conclude that C. versicolorbiomass had a bifidogenic effect and, therefore, could be interesting to its establishment as a prebiotic.

English

ISBN: 9798381541779Subjects--Topical Terms:

3540520
Pathogens.

Evaluation of the Prebiotic Potential of Coriolus Versicolor : Effect upon the Human Gut Microbiota /
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520 $a The human gut microbiota has become increasingly relevant when considering human health and disease due to its connection with the immune system and human metabolism. One way of actively modulating the gut microbiota is through the ingestion of prebiotics, that can be fermented by beneficial members of the colonic population, resulting in the production of certain metabolites regarded as beneficial, such as Short Chain Fatty Acids (SCFA). Coriolus versicolor is a mushroom that, while known for its immunomodulatory and anticarcinogenic properties has also been previously described as possessing some prebiotic potential. However, most studies focus on the potentialities of C. versicolor extracts, leaving the properties of its biomass unstudied. Hence, the main goal of the present work was to evaluate the prebiotic potential in a commercial supplement of C. versicolorbiomass using an in vitro faecal model. In order to achieve the proposed goal, the biomass was first submitted to a digestion simulation after which, it was used to perform human faecal fermentations using faeces from five distinct donors. Samples were collected at 0, 12, 24 and 48 h, for pH measurements and to be characterized regarding organic acid production, with emphasis on SCFA (through HPLC), as well as characterize the microbial profile (through qPCR).Despite the intrinsic variability of the human gut microbiota between individuals, the presence of C. versicolor biomass consistently resulted in an increase in the number of Bifidobacterium's 16S rRNA gene copies, for all donors when comparing to the negative control, although the overall increase was lower than the one registered for the positive control (i.e. FOS). This behaviour was observed after 24 h (negative control- 0.203 ± 0.017; positive control- 0.282 ± 0.011; C. versicolor- 0.238 ± 0.008, expressed in log of copy numbers of 16S rRNA per ng of DNA) and 48 h (negative control- 0.212 ± 0.016; positive control- 0.301 ± 0.027; C. versicolor- 0.268 ± 0.011, expressed in log of copy numbers of 16S rRNA per ng of DNA), but no significant differences were found between the assayed conditions after 12 h incubation. For the remaining bacterial groups characterized (Firmicutes, Bacteroidetes, Bacteroides, Clostridium leptum and Lactobacillus) it was not possible to observe a general trend but only donor specific behaviours. Overall, both C. versicolor and FOS resulted in a decrease of the pH values, in opposition to the negative control, which confirmed the production of acids as a result of bacterial metabolism. As for SCFA and lactic acid quantification, at the end of the fermentation, C. versicolor biomass led to the production of acetic acid (0.212 ± 0.088 mg mL-1), lactic acid (0.032 ± 0.002 mg mL-1) and propionic acid (0.0184 ± 0.007 mg mL-1). In sum, it is possible to conclude that C. versicolorbiomass had a bifidogenic effect and, therefore, could be interesting to its establishment as a prebiotic.
520 $a A microbiota intestinal humana tem-se tornado progressivamente mais relevante no estudo da saude humana e de certas doencas, devido a sua ligacao com o sistema imunitario e com o metabolismo humano. Um modo de modelar a microbiota intestinal e atraves da ingestao de prebioticos, que podem ser fermentados por bacterias beneficas presentes no colon, resultando na producao de certos metabolitos considerados benignos, tais como os acidos gordos de cadeia curta. O Coriolus versicolor e um cogumelo que, embora seja conhecido pelas suas propriedades imunomoduladoras e anticarcinogenicas, tambem foi anteriormente associado a uma potencial atividade prebiotica. No entanto, os estudos publicados focam se nas potencialidades dos extratos de C. versicolor, enquanto as propriedades da biomassa permanecem pouco estudadas. Assim, o principal objetivo do presente trabalho foi a avaliacao do potencial prebiotico de um suplemento comercial de biomassa de C. versicolor, utilizando um modelo fecalin vitro. A fim de atingir o objetivo proposto, a biomassa foi primeiramente submetida a uma simulacao do processo digestivo, apos a qual esta foi utilizada para realizar fermentacoes usando fezes de cinco dadores distintos. Durante a fermentacao foram recolhidas amostras as 0, 12, 24, e 48 h para realizar medicoes dos valores de pH e de as caracterizar relativamente a producao de acidos organicos, particularmente, os acidos gordos de cadeia curta (atraves de HPLC) e ao seu perfil microbiano (atraves de qPCR).Apesar da variabilidade intrinseca associada a microbiota intestinal humana entre individuos, a presenca da biomassa de C. versicolor resultou num aumento do numero de copias do gene 16S rRNA de Bifidobacterium para todos os dadores, em comparacao com o controlo negativo, ainda que o aumento global tenha sido inferior ao do controlo positivo (FOS). Este comportamento foi observado apos 24 h (controlo negativo- 0,203 ± 0,017; controlo positivo- 0,282 ± 0,011; C. versicolor- 0,238 ± 0,008, expresso como log do numero de copias de 16S rRNA por ng de ADN) e apos 48 h (controlo negativo- 0,212 ± 0,016; controlo positivo - 0,301 ± 0,027; C. versicolor- 0,268 ± 0,011, expresso como log do numero de copias de 16S rRNA por ng de ADN), mas nao foram encontradas diferencas significativas entre as condicoes testadas apos 12 h de incubacao. Relativamente a outros grupos bacterianos (Firmicutes, Bacteroidetes, Bacteroides, Clostridium leptum e Lactobacillus), nao foi possivel estabelecer uma tendencia geral, mas apenas comportamentos especificos de cada dador. De um modo geral, quer o C. versicolor quer o FOS, resultaram numa diminuicao dos valores de pH, por oposicao ao controlo negativo, o que confirmou a producao de acidos, como consequencia do metabolismo bacteriano. No que se refere a quantificacao dos acidos gordos de cadeia curta e do acido lactico, no fim da fermentacao, a biomassa de C. versicolor levou a producao de acido acetico (0,212 ± 0,088 mg mL-1), seguida de acido lactico (0,032 ± 0,002 mg mL-1), e do acido propionico (0,018 ± 0,007 mg mL-1). Em suma, e possivel concluir que a biomassa de C. versciolor teve um efeito bifidogenico, relevante na demonstracao do seu potencial prebiotico.
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