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Biofabrication of Hierarchical and C...

Brunel, Lucia G.,

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Biofabrication of Hierarchical and Cell-instructive Biomaterials for Tissue Engineering /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Biofabrication of Hierarchical and Cell-instructive Biomaterials for Tissue Engineering // Lucia G Brunel.
作者:	Brunel, Lucia G.,
面頁冊數:	1 electronic resource (220 pages)
附註:	Source: Dissertations Abstracts International, Volume: 86-08, Section: B.
Contained By:	Dissertations Abstracts International86-08B.
標題:	3-D printers. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31901318
ISBN:	9798311948456

Biofabrication of Hierarchical and Cell-instructive Biomaterials for Tissue Engineering /
Brunel, Lucia G.,

Biofabrication of Hierarchical and Cell-instructive Biomaterials for Tissue Engineering /Lucia G Brunel. - 1 electronic resource (220 pages)

Source: Dissertations Abstracts International, Volume: 86-08, Section: B.

The demand for tissue and organ transplants to treat patients with organ failure, traumatic injuries, and degenerative diseases severely outpaces the supply of donor tissue, necessitating bioengineered strategies. In the case of corneal blindness, which affects over 12.5 million individuals globally, less than 2% of patients have access to human donor tissue for corneal transplantation. To address this critical gap between the supply and demand for human donor tissue, three-dimensional (3D) bioprinting has emerged as a powerful technology for patterning bioinks-composed of living cells and extracellular matrix components-into bespoke constructs that resemble the form and function of native tissues. The cornea serves as an ideal early case study for 3D bioprinting, due to its relatively simple tissue structure and the pressing clinical demand for engineered corneal substitutes. In this thesis, I first describe the design of a collagen-based bioink for corneal tissue engineering with the appropriate mechanical, optical, and biological properties to promote regeneration, as validated through pre-clinical studies in rabbits. This bioink allows for the delivery of corneal stem cells to corneal wounds within a matrix that restores the curvature of the cornea, remains transparent, and promotes rapid and healthy wound closure. I next demonstrate the adaptation of this bioink for embedded 3D bioprinting. We achieve hierarchical, multi-length-scale patterning by leveraging fundamental fluid dynamics: The bioink mixes with sacrificial microparticles that comprise the support bath, introducing microscopic internal porosity to the prints. As an alternative approach to manipulate the hierarchical features of the bioink, I finally introduce fibers into the material by creating an interpenetrating network of collagen crosslinked with two independent mechanisms. By incorporating hierarchical features (either void spaces or fibers) into the engineered matrices, I demonstrate the important interplay between material chemistry and physical structure for guiding the behavior of encapsulated corneal stem cells. Collectively, these works offer insights into the design of hierarchical and cell-instructive biomaterials for tissue engineering and biofabrication.

English

ISBN: 9798311948456Subjects--Topical Terms:

3681750
3-D printers.

Biofabrication of Hierarchical and Cell-instructive Biomaterials for Tissue Engineering /
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