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Biocatalytic Performance of Carbonic...

Ayaz, Ilyas Umar,

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Biocatalytic Performance of Carbonic Anhydrase Immobilized Within Polyurethane Foam in Water-Miscible Organic Solvents = = Poli̇uretan Sungere IMmobi̇li̇ze Edi̇lmi̇s Karboni̇k Anhi̇drazin Suyla Karisabi̇len Organi̇k CozuculerdeBi̇yokatali̇zleme Performansi /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Biocatalytic Performance of Carbonic Anhydrase Immobilized Within Polyurethane Foam in Water-Miscible Organic Solvents =/ Ilyas Umar Ayaz.
其他題名:	Poli̇uretan Sungere IMmobi̇li̇ze Edi̇lmi̇s Karboni̇k Anhi̇drazin Suyla Karisabi̇len Organi̇k CozuculerdeBi̇yokatali̇zleme Performansi /
作者:	Ayaz, Ilyas Umar,
面頁冊數:	1 electronic resource (85 pages)
附註:	Source: Masters Abstracts International, Volume: 83-02.
Contained By:	Masters Abstracts International83-02.
標題:	Surfactants. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28478072
ISBN:	9798505508411

Biocatalytic Performance of Carbonic Anhydrase Immobilized Within Polyurethane Foam in Water-Miscible Organic Solvents = = Poli̇uretan Sungere IMmobi̇li̇ze Edi̇lmi̇s Karboni̇k Anhi̇drazin Suyla Karisabi̇len Organi̇k CozuculerdeBi̇yokatali̇zleme Performansi /
Ayaz, Ilyas Umar,

Biocatalytic Performance of Carbonic Anhydrase Immobilized Within Polyurethane Foam in Water-Miscible Organic Solvents =Poli̇uretan Sungere IMmobi̇li̇ze Edi̇lmi̇s Karboni̇k Anhi̇drazin Suyla Karisabi̇len Organi̇k CozuculerdeBi̇yokatali̇zleme Performansi /Ilyas Umar Ayaz. - 1 electronic resource (85 pages)

Source: Masters Abstracts International, Volume: 83-02.

The effects of water-miscible organic solvents such as acetonitrile and ethanol on the activity of free and immobilized bovine carbonic anhydrase (CA) were investigated. The CA was covalently immobilized within polyurethane (PU) foam by cross-linking. Although PU foam holds water almost 12 times of its weight, it was found that adsorption isotherm of moisture on PU foam was a Type III indicating that water and PU foam were non-interacting to each other. The activities for the free and immobilized CA were estimated using para-nitrophenyl acetate (p-NPA) as the substrate. The enzyme activities were estimated in increasing volume percents of organic solvent in Tris buffer (10-90%). p-NP, which is one of the products of the hydrolysis reaction of p-NPA, was characterized in the presence of organic solvents and it was observed that its aborptivities were decreased significantly as the organic solvent percentages were increased indicating that p-NP and the water-miscible organic solvent form a complex through mostly a hydrogen bonding. The free CA showed decreasing activity up to critical percentages of organic solvent (40-60%), and then exhibited an increasing activity. The immobilized CA showed decreasing activity in acetonitrile at percentages up to 50%, and then lost its total activity at higher acetonitrile percentages, however, the immobilized CA exhibited no activity in ethanol at percentages above 10%. Stability tests showed that the immobilized CA was dramatically inactivated in the organic solvents at percentages above 30% in shorter times. It was concluded that the water-miscible organic solvents severely perturbed the active site of the enzyme, thus denaturating the enzyme.

English

ISBN: 9798505508411Subjects--Topical Terms:

3560257
Surfactants.

Biocatalytic Performance of Carbonic Anhydrase Immobilized Within Polyurethane Foam in Water-Miscible Organic Solvents = = Poli̇uretan Sungere IMmobi̇li̇ze Edi̇lmi̇s Karboni̇k Anhi̇drazin Suyla Karisabi̇len Organi̇k CozuculerdeBi̇yokatali̇zleme Performansi /
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245 1 0 $a Biocatalytic Performance of Carbonic Anhydrase Immobilized Within Polyurethane Foam in Water-Miscible Organic Solvents = $b Poli̇uretan Sungere IMmobi̇li̇ze Edi̇lmi̇s Karboni̇k Anhi̇drazin Suyla Karisabi̇len Organi̇k CozuculerdeBi̇yokatali̇zleme Performansi / $c Ilyas Umar Ayaz.
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300 $a 1 electronic resource (85 pages)
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500 $a Source: Masters Abstracts International, Volume: 83-02.
500 $a Advisors: Ozdemir, Ekrem; Okvur, Devrim Pesen Committee members: Ozdemir, Ekrem; Okvur, Devirm Pesen; Bayraktar, Oguz; Ozsen, Ash Yuksel; sanli, Gulsah.
502 $b M.Sc. $c Izmir Institute of Technology (Turkey) $d 2013.
520 $a The effects of water-miscible organic solvents such as acetonitrile and ethanol on the activity of free and immobilized bovine carbonic anhydrase (CA) were investigated. The CA was covalently immobilized within polyurethane (PU) foam by cross-linking. Although PU foam holds water almost 12 times of its weight, it was found that adsorption isotherm of moisture on PU foam was a Type III indicating that water and PU foam were non-interacting to each other. The activities for the free and immobilized CA were estimated using para-nitrophenyl acetate (p-NPA) as the substrate. The enzyme activities were estimated in increasing volume percents of organic solvent in Tris buffer (10-90%). p-NP, which is one of the products of the hydrolysis reaction of p-NPA, was characterized in the presence of organic solvents and it was observed that its aborptivities were decreased significantly as the organic solvent percentages were increased indicating that p-NP and the water-miscible organic solvent form a complex through mostly a hydrogen bonding. The free CA showed decreasing activity up to critical percentages of organic solvent (40-60%), and then exhibited an increasing activity. The immobilized CA showed decreasing activity in acetonitrile at percentages up to 50%, and then lost its total activity at higher acetonitrile percentages, however, the immobilized CA exhibited no activity in ethanol at percentages above 10%. Stability tests showed that the immobilized CA was dramatically inactivated in the organic solvents at percentages above 30% in shorter times. It was concluded that the water-miscible organic solvents severely perturbed the active site of the enzyme, thus denaturating the enzyme.
520 $a Asetonitril ve etanol gibi suyla karisabilen organik cozuculerin serbest veimmobilize edilmis karbonik anhidraz enziminin aktivitesine etkileri arastirildi.Karbonik anhidraz enzimi, poliuretan sunger icerisine kovalent capraz baglanma ileimmobilize edildi. Poliuretan sunger kendi agirliginin yaklasik 12 kati kadar sututabilmesine ragmen, poliuretan sungerin uzerindeki nemin adsorpsiyon izoterminin,Tip III adsorpsiyon izotermi oldugu bulundu ve bu durum, poliuretan sunger ile suyunbirbirleriyle cok az etkilestigini gosterdi. Serbest ve immobilize karbonik anhidrazenziminin aktiviteleri, substrat olarak para-nitrofenil asetat kullanilarak olculdu. Enzimaktiviteleri, Tris tampon cozeltisindeki organik cozucunun hacimsel yuzdesi arttirilarakhesaplandi (%10-90). Para-nitrofenil asetatin hidroliz reaksiyonu urunlerinden biri olanpara-nitrofenol, organik cozuculerde karakterize edildi. Para-nitrofenolunabsorptivitesinin, karisimdaki organik cozucu yuzdeleri arttirildiginda onemli olcudedustugu gozlemlendi. Bu durum, para-nitrofenol ve suyla karisabilen organikcozuculerin daha cok hidrojen baglari ile kompleks bir yapi olusturdugunu gosterdi.Serbest enzim, kritik organik cozucu yuzdelerine (%40-60) kadar aktivite dususu vedaha sonra, yuksek organik cozucu yuzdelerinde aktivite artisi gosterdi. Immobilizeenzim, %50'ye kadarki asetonitril yuzdelerinde aktivite dususu gosterdi ve daha sonra,yuksek asetonitril yuzdelerinde butun aktivitesini kaybetti. %10'dan yuksek etanolyuzdelerinde ise immobilize enzim hicbir aktivite gostermedi. Stabilite testlerindeimmobilize enzim, %30'tan yuksek organik cozucu yuzdelerinde, carpici bir bicimdekisa zamanda inaktive oldu. Sonuc olarak, suyla karisabilen organik cozuculer, enziminaktif bolgesinin yapisini ciddi bir bicimde bozarak enzimi denature etti.
546 $a English
590 $a School code: 2215
650 4 $a Surfactants. $3 3560257
650 4 $a Disadvantages. $3 3684692
650 4 $a Hydrogen bonds. $3 768332
650 4 $a Biocatalysts. $3 3683849
650 4 $a Chemical reactions. $3 519248
650 4 $a Polymerization. $3 560492
650 4 $a Binding sites. $3 3560349
650 4 $a Adsorption. $3 580046
650 4 $a Amino acids. $3 558768
650 4 $a Energy. $3 876794
650 4 $a Lipids. $3 558980
650 4 $a Hydration. $3 738666
650 4 $a Ethanol. $3 1613481
650 4 $a Polymers. $3 535398
650 4 $a Solvents. $3 620946
650 4 $a Flexibility. $3 3560705
650 4 $a Porous materials. $3 719049
650 4 $a Chemical bonds. $3 560512
650 4 $a Catalysis. $3 560465
650 4 $a Polypeptides. $3 1085124
650 4 $a Polymer chemistry. $3 3173488
650 4 $a Atomic physics. $3 3173870
690 $a 0748
690 $a 0791
690 $a 0495
710 2 $a Izmir Institute of Technology (Turkey). $e degree granting institution. $3 3823866
720 1 $a Ozdemir, Ekrem $e degree supervisor.
720 1 $a Okvur, Devrim Pesen $e degree supervisor.
773 0 $t Masters Abstracts International $g 83-02.
790 $a 2215
791 $a M.Sc.
792 $a 2013
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28478072