東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Studies of Mumps Virus RNA Synthesis...

Briggs, Kelsey Rose,

Linked to FindBook

Google Book

Amazon

博客來

Studies of Mumps Virus RNA Synthesis and Immunogenicity /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Studies of Mumps Virus RNA Synthesis and Immunogenicity // Kelsey Rose Briggs.
Author:	Briggs, Kelsey Rose,
Description:	1 electronic resource (197 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 83-08, Section: B.
Contained By:	Dissertations Abstracts International83-08B.
Subject:	Virology. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28774709
ISBN:	9798780624073

Studies of Mumps Virus RNA Synthesis and Immunogenicity /
Briggs, Kelsey Rose,

Studies of Mumps Virus RNA Synthesis and Immunogenicity /Kelsey Rose Briggs. - 1 electronic resource (197 pages)

Source: Dissertations Abstracts International, Volume: 83-08, Section: B.

Mumps virus (MuV) is a highly contagious human pathogen in the Paramyxoviridae family. The hallmark symptom of mumps is the swelling of the parotid glands. Though clinical symptoms of mumps are often mild, severe neurological complications, such as meningitis and encephalitis, can occur (1). After the introduction of the mumps vaccine, cases dramatically decreased to less than 500 per year in the United States. However, there has been a resurgence of mumps in the last two decades, warranting a better understanding of the disease and virus (2). There are no approved anti-viral drugs available for mumps. Viral replication and transcription have become targets for therapeutics in recent years (3). This work seeks to better understand virus-host interactions and the role phosphorylation plays in RNA synthesis. It is well established that the MuV nucleoprotein (NP) and phosphoprotein (P) are highly phosphorylated by host kinases and that their phosphorylation status regulates viral transcription and replication (4, 5). However, many host kinase regulators remain unknown. In this study, we identify a novel virus-host interaction. We show that host kinase, ribosomal protein S6 kinase beta-1 (RPS6KB1), negatively regulates MuV growth via phosphorylation of the MuV phosphoprotein. We then provide novel evidence that the MuV large (L) protein is phosphorylated during in vitro infection and identify four putative phosphorylation sites within the polyribonucleotidyltransferase domain of L. Our work provides insight into phosphorylation as a regulator of MuV growth. MuV outbreaks are occurring in vaccinated populations, and clinical isolates sequenced from these outbreaks belong to a divergent genotype (genotype G) compared to the vaccine strain (genotype A), which questions the efficacy of the mumps component of the measles, mumps, and rubella (MMR) vaccine. (2). We examine the immunogenicity and longevity of recombinant genotype G-based vaccines and show that a third dose of a genotype G-based vaccine increases neutralizing titers toward the circulating outbreak strain of MuV. We identify a host kinase important for MuV replication, elucidate MuV polymerase phosphorylation, and seek to advance the development of a next generation MuV vaccine.

English

ISBN: 9798780624073Subjects--Topical Terms:

642304
Virology.
Subjects--Index Terms:

Large protein

Studies of Mumps Virus RNA Synthesis and Immunogenicity /
LDR:03620nmm a22004093i 4500 001 2433990
005 20250905091321.5
006 m o d
007 cr|nu||||||||
008 261011s2021 miu||||||m |||||||eng d
020 $a 9798780624073
035 $a (MiAaPQD)AAI28774709
035 $a AAI28774709
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Briggs, Kelsey Rose, $e author. $0 (orcid)0000-0001-8368-5487 $3 3823816
245 1 0 $a Studies of Mumps Virus RNA Synthesis and Immunogenicity / $c Kelsey Rose Briggs.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 1 electronic resource (197 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 83-08, Section: B.
500 $a Advisors: He, Biao Committee members: Brindley, Melinda ; Watford, Wendy ; Rada, Balazs ; Rota , Paul.
502 $b Ph.D. $c University of Georgia $d 2021.
520 $a Mumps virus (MuV) is a highly contagious human pathogen in the Paramyxoviridae family. The hallmark symptom of mumps is the swelling of the parotid glands. Though clinical symptoms of mumps are often mild, severe neurological complications, such as meningitis and encephalitis, can occur (1). After the introduction of the mumps vaccine, cases dramatically decreased to less than 500 per year in the United States. However, there has been a resurgence of mumps in the last two decades, warranting a better understanding of the disease and virus (2). There are no approved anti-viral drugs available for mumps. Viral replication and transcription have become targets for therapeutics in recent years (3). This work seeks to better understand virus-host interactions and the role phosphorylation plays in RNA synthesis. It is well established that the MuV nucleoprotein (NP) and phosphoprotein (P) are highly phosphorylated by host kinases and that their phosphorylation status regulates viral transcription and replication (4, 5). However, many host kinase regulators remain unknown. In this study, we identify a novel virus-host interaction. We show that host kinase, ribosomal protein S6 kinase beta-1 (RPS6KB1), negatively regulates MuV growth via phosphorylation of the MuV phosphoprotein. We then provide novel evidence that the MuV large (L) protein is phosphorylated during in vitro infection and identify four putative phosphorylation sites within the polyribonucleotidyltransferase domain of L. Our work provides insight into phosphorylation as a regulator of MuV growth. MuV outbreaks are occurring in vaccinated populations, and clinical isolates sequenced from these outbreaks belong to a divergent genotype (genotype G) compared to the vaccine strain (genotype A), which questions the efficacy of the mumps component of the measles, mumps, and rubella (MMR) vaccine. (2). We examine the immunogenicity and longevity of recombinant genotype G-based vaccines and show that a third dose of a genotype G-based vaccine increases neutralizing titers toward the circulating outbreak strain of MuV. We identify a host kinase important for MuV replication, elucidate MuV polymerase phosphorylation, and seek to advance the development of a next generation MuV vaccine.
546 $a English
590 $a School code: 0077
650 4 $a Virology. $3 642304
650 4 $a Immunology. $3 611031
653 $a Large protein
653 $a Mumps
653 $a Phosphoprotein
653 $a Vaccine
690 $a 0720
690 $a 0982
710 2 $a University of Georgia. $b Infectious Diseases - PHD. $e degree granting institution. $3 3823817
720 1 $a He, Biao $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 83-08B.
790 $a 0077
791 $a Ph.D.
792 $a 2021
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28774709