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From Microalgae to Sustainable Plast...

Wen, Yidan Daniella,

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From Microalgae to Sustainable Plastics: Preparing Protein Aggregates From Algal Biomass /

Record Type:	Electronic resources : Monograph/item
Title/Author:	From Microalgae to Sustainable Plastics: Preparing Protein Aggregates From Algal Biomass // Yidan Daniella Wen.
Author:	Wen, Yidan Daniella,
Description:	1 electronic resource (68 pages)
Notes:	Source: Masters Abstracts International, Volume: 86-11.
Contained By:	Masters Abstracts International86-11.
Subject:	Plant-based foods. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31923420
ISBN:	9798311908504

From Microalgae to Sustainable Plastics: Preparing Protein Aggregates From Algal Biomass /
Wen, Yidan Daniella,

From Microalgae to Sustainable Plastics: Preparing Protein Aggregates From Algal Biomass /Yidan Daniella Wen. - 1 electronic resource (68 pages)

Source: Masters Abstracts International, Volume: 86-11.

This thesis investigates the extraction and purification of proteins from microalgae, specifically spirulina and chlorella, and their self-assembly into amyloid-like aggregates as building blocks towards the development of sustainable bioplastic materials. In response to growing environmental concerns over traditional plastics, amyloid fibers - highly organized protein aggregates - offer a potential alternative due to their structural stability, functional versatility, and sustainability. Although amyloid fibers have been prepared from animal- and plant-based protein sources, microalgae protein-derived amyloid fibers remain largely unexplored. Given their high protein content and the current industrial production of microalgae, spirulina and chlorella present an untapped resource for the development of such materials.Protein extraction and purification from spirulina and chlorella was performed using a pH shifting method, which is well-documented as an efficient, facile, and high-throughput protein extraction technique. Amyloid-like aggregation was induced under acidic conditions and elevated temperature, whereby spirulina proteins formed beta-sheet-rich structures with the typical fiber-like morphology, while chlorella proteins predominantly aggregated into non-fibrillar, spherical/annular structures. This difference in structural morphology underscores the importance of both protein purity and composition between sources. Protein aggregation is investigated in detail through Thioflavin T fluorescence, Fourier Transform Infrared and circular dichroism spectroscopies, dynamic light scattering, zeta potential measurements, and atomic force microscopy imaging. Despite their morphological differences, both microalgal protein aggregates exhibit impressive stability across a wide pH range, persisting up to pH 11 before disassembly at pH 12, making them promising candidates for robust sustainable applications. Preliminary investigations incorporating model amyloid fibers into crude spirulina biomass revealed minimal improvement in mechanical strength, attributed to poor component mixing, underscoring the importance of processing in composite formation.In short, this work underscores the feasibility of transforming microalgae proteins into stable aggregates with complex morphologies, and highlights the importance of biomass source, protein purity, and composition on the aggregation process. Our findings contribute to the growing and exceedingly important field of bio-based and biodegradable materials, and offer insights into the fundamental principles governing amyloid fiber formation. We anticipate that these efforts will lead to the formation of high-performance, tunable bioplastics which can be used for a variety of different applications.

English

ISBN: 9798311908504Subjects--Topical Terms:

3823707
Plant-based foods.
Subjects--Index Terms:

Microalgae

From Microalgae to Sustainable Plastics: Preparing Protein Aggregates From Algal Biomass /
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500 $a Advisors: de France, Kevin.
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520 $a This thesis investigates the extraction and purification of proteins from microalgae, specifically spirulina and chlorella, and their self-assembly into amyloid-like aggregates as building blocks towards the development of sustainable bioplastic materials. In response to growing environmental concerns over traditional plastics, amyloid fibers - highly organized protein aggregates - offer a potential alternative due to their structural stability, functional versatility, and sustainability. Although amyloid fibers have been prepared from animal- and plant-based protein sources, microalgae protein-derived amyloid fibers remain largely unexplored. Given their high protein content and the current industrial production of microalgae, spirulina and chlorella present an untapped resource for the development of such materials.Protein extraction and purification from spirulina and chlorella was performed using a pH shifting method, which is well-documented as an efficient, facile, and high-throughput protein extraction technique. Amyloid-like aggregation was induced under acidic conditions and elevated temperature, whereby spirulina proteins formed beta-sheet-rich structures with the typical fiber-like morphology, while chlorella proteins predominantly aggregated into non-fibrillar, spherical/annular structures. This difference in structural morphology underscores the importance of both protein purity and composition between sources. Protein aggregation is investigated in detail through Thioflavin T fluorescence, Fourier Transform Infrared and circular dichroism spectroscopies, dynamic light scattering, zeta potential measurements, and atomic force microscopy imaging. Despite their morphological differences, both microalgal protein aggregates exhibit impressive stability across a wide pH range, persisting up to pH 11 before disassembly at pH 12, making them promising candidates for robust sustainable applications. Preliminary investigations incorporating model amyloid fibers into crude spirulina biomass revealed minimal improvement in mechanical strength, attributed to poor component mixing, underscoring the importance of processing in composite formation.In short, this work underscores the feasibility of transforming microalgae proteins into stable aggregates with complex morphologies, and highlights the importance of biomass source, protein purity, and composition on the aggregation process. Our findings contribute to the growing and exceedingly important field of bio-based and biodegradable materials, and offer insights into the fundamental principles governing amyloid fiber formation. We anticipate that these efforts will lead to the formation of high-performance, tunable bioplastics which can be used for a variety of different applications.
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590 $a School code: 0283
650 4 $a Plant-based foods. $3 3823707
650 4 $a Heavy metals. $3 552237
650 4 $a Seeds. $3 573605
650 4 $a Microbiology. $3 536250
650 4 $a Biochemistry. $3 518028
653 $a Microalgae
653 $a Protein aggregates
690 $a 0487
690 $a 0410
710 2 $a Queen's University (Canada). $e degree granting institution. $3 3765623
720 1 $a de France, Kevin $e degree supervisor.
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