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Development of in Vitro and Transfor...

Mkhonza, Nompumelelo Lucritia,

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Development of in Vitro and Transformation Methods for Sorghum Bicolor (L. Moench) /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development of in Vitro and Transformation Methods for Sorghum Bicolor (L. Moench) // Nompumelelo Lucritia Mkhonza.
Author:	Mkhonza, Nompumelelo Lucritia,
Description:	1 electronic resource (172 pages)
Notes:	Source: Masters Abstracts International, Volume: 83-02.
Contained By:	Masters Abstracts International83-02.
Subject:	Infections. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28330395
ISBN:	9798744428471

Development of in Vitro and Transformation Methods for Sorghum Bicolor (L. Moench) /
Mkhonza, Nompumelelo Lucritia,

Development of in Vitro and Transformation Methods for Sorghum Bicolor (L. Moench) /Nompumelelo Lucritia Mkhonza. - 1 electronic resource (172 pages)

Source: Masters Abstracts International, Volume: 83-02.

Sorghum [Sorghum bicolor (L.)] is classified as a relatively recalcitrant crop due to its poor amenability to in vitro and genetic manipulation. An efficient and reproducible in vitro plant regeneration method is vital for a successful transformation of any crop. Plant regeneration and transformation of eight selected elite sorghum genotypes was studied. Immature zygotic embryos were used as explants and cultured on two different callus induction media. Three genotypes ICSV1111N, SRN39 and P898012 were found to be highly regenerable producing 5.99; 5.1 and 4.74 regenerants per explant respectively on the G2+L-proline callus induction medium. The eight elite sorghum genotypes were co-bombarded with the uidA reporter gene and manA selectable marker gene. Bombarded immature zygotic embryos were selected on G2+L-proline callus induction medium supplemented with mannose as a selective agent. PCR Positive transformants were only obtained from genotype P898012. Furthermore the genotype P898012 was stably transformed with a lower DNA amount of manA minimal transgene. The manA gene presence was confirmed with PCR and southern blot analyses and a transformation efficiency of 0.38% was attained. The fertile transgenic plants displayed simple integration patterns, and the gene was also inherited to the T1 progeny of manA resistant trasnsformants in a Mendelian fashion.

English

ISBN: 9798744428471Subjects--Topical Terms:

1621997
Infections.

Development of in Vitro and Transformation Methods for Sorghum Bicolor (L. Moench) /
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100 1 $a Mkhonza, Nompumelelo Lucritia, $e author. $3 3821772
245 1 0 $a Development of in Vitro and Transformation Methods for Sorghum Bicolor (L. Moench) / $c Nompumelelo Lucritia Mkhonza.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2009
300 $a 1 electronic resource (172 pages)
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337 $a computer $b c $2 rdamedia
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500 $a Source: Masters Abstracts International, Volume: 83-02.
500 $a Advisors: Alagiozoglou, Lee; Mehlo, Luke; Grootboom, Andile.
502 $b M.Tech. $c University of Johannesburg (South Africa) $d 2009.
520 $a Sorghum [Sorghum bicolor (L.)] is classified as a relatively recalcitrant crop due to its poor amenability to in vitro and genetic manipulation. An efficient and reproducible in vitro plant regeneration method is vital for a successful transformation of any crop. Plant regeneration and transformation of eight selected elite sorghum genotypes was studied. Immature zygotic embryos were used as explants and cultured on two different callus induction media. Three genotypes ICSV1111N, SRN39 and P898012 were found to be highly regenerable producing 5.99; 5.1 and 4.74 regenerants per explant respectively on the G2+L-proline callus induction medium. The eight elite sorghum genotypes were co-bombarded with the uidA reporter gene and manA selectable marker gene. Bombarded immature zygotic embryos were selected on G2+L-proline callus induction medium supplemented with mannose as a selective agent. PCR Positive transformants were only obtained from genotype P898012. Furthermore the genotype P898012 was stably transformed with a lower DNA amount of manA minimal transgene. The manA gene presence was confirmed with PCR and southern blot analyses and a transformation efficiency of 0.38% was attained. The fertile transgenic plants displayed simple integration patterns, and the gene was also inherited to the T1 progeny of manA resistant trasnsformants in a Mendelian fashion.
546 $a English
590 $a School code: 2140
650 4 $a Infections. $3 1621997
650 4 $a Dihydrofolate reductase. $3 3558617
650 4 $a Agricultural production. $3 3559355
650 4 $a Food. $3 551593
650 4 $a Flowers & plants. $3 3564028
650 4 $a Corn. $3 661768
650 4 $a Amino acids. $3 558768
650 4 $a Crops. $3 672677
650 4 $a Metabolism. $3 541349
650 4 $a Transgenic plants. $3 525755
650 4 $a Nitrogen. $3 1314426
650 4 $a Plasmids. $3 3319070
650 4 $a Carbon. $3 604057
650 4 $a Cloning. $3 571606
650 4 $a Wheat. $3 660846
650 4 $a Seeds. $3 573605
650 4 $a Genetic engineering. $3 530509
650 4 $a Rice. $3 649844
650 4 $a Abiotic stress. $3 3685293
650 4 $a Viral infections. $3 3540521
650 4 $a Tumors. $3 893964
650 4 $a Plant growth. $3 3540341
650 4 $a Cell division. $3 816756
650 4 $a Barley. $3 3564839
650 4 $a Agronomy. $3 2122783
650 4 $a Genetics. $3 530508
650 4 $a Botany. $3 516217
650 4 $a Agriculture. $3 518588
650 4 $a Cellular biology. $3 3172791
650 4 $a Bioengineering. $3 657580
650 4 $a Engineering. $3 586835
650 4 $a Morphology. $3 591167
690 $a 0287
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690 $a 0285
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690 $a 0309
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710 2 $a University of Johannesburg (South Africa). $e degree granting institution. $3 3759059
720 1 $a Alagiozoglou, Lee $e degree supervisor.
720 1 $a Mehlo, Luke $e degree supervisor.
720 1 $a Grootboom, Andile $e degree supervisor.
773 0 $t Masters Abstracts International $g 83-02.
790 $a 2140
791 $a M.Tech.
792 $a 2009
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28330395