東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

The Role and Regulation of ERK/MAPK ...

Robinson-Thiewes, Sarah,

Linked to FindBook

Google Book

Amazon

博客來

The Role and Regulation of ERK/MAPK in the C. elegans Germline /

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Role and Regulation of ERK/MAPK in the C. elegans Germline // Sarah Robinson-Thiewes.
Author:	Robinson-Thiewes, Sarah,
Description:	1 electronic resource (155 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 82-07, Section: B.
Contained By:	Dissertations Abstracts International82-07B.
Subject:	Genetics. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28262393
ISBN:	9798557026048

The Role and Regulation of ERK/MAPK in the C. elegans Germline /
Robinson-Thiewes, Sarah,

The Role and Regulation of ERK/MAPK in the C. elegans Germline /Sarah Robinson-Thiewes. - 1 electronic resource (155 pages)

Source: Dissertations Abstracts International, Volume: 82-07, Section: B.

Orchestrated gene expression and development are intimately linked. From embryogenesis to tissue homeostasis, cells must control the abundance and timing of gene expression-especially of key developmental factors. An improperly expressed gene may usurp control and drive a cell, tissue, or organism to ruin. Thus, understanding the in vivo role and regulation of critical developmental genes is required. The Caenorhabditis elegans germline is a valuable tool to analyze in vivo germ cell development. This work focuses on ERK/MAPK and the regulatory steps of transcriptional progression. The single C. elegans MAPK ortholog MPK-1 has multiple roles in germline development including sperm/oocyte decision, meiotic progression, and oogenesis. The mpk-1 locus encodes two isoforms, mpk-1a and mpk-1b. The mpk-1b isoform has an exclusive first exon and long first intron; mpk-1a shares all other coding regions with mpk-1b. Each isoform has tissue specific expression: MPK-1B is germline specific and MPK-1A is somatic specific. Despite different tissue localizations, each MPK-1 isoform has a role in germline development. MPK-1B promotes oogenesis and fertility; MPK-1A promotes germline stem cell proliferation and meiotic progression from the soma. Thus, MPK-1 can autonomously and non-autonomously affect germ cell development. Using smFISH (single molecule FISH) targeting either mpk-1b's long first intron or all coding exons, the nascent transcripts at active transcription sites (ATS) and mRNA can be visualized along the germline developmental axis. Surprisingly, the ATS of mpk-1 and Notch components lag-1 and lag-3 exist as either of two classes: the incomplete ATS (iATS) and complete ATS (cATS). The iATS generate incomplete nascent transcripts and cATS generate complete nascent transcripts; therefore, the ATS classes represent different extents of transcriptional progression. Only the cATS frequency correlates with the graded nascent transcripts and mRNA abundance. Furthermore, the iATS and cATS frequencies are graded in a gene-, stage-, and sex-specific manner along the germline. For example, the iATS frequency of mpk-1 and lag-3 is high in the GSC pool whereas lag-1 cATS frequency is high in the same cells. Once cells begin overt differentiation, all three genes have a high cATS frequency. Thus, regulated transcriptional progression is critical for gene expression during development.

English

ISBN: 9798557026048Subjects--Topical Terms:

530508
Genetics.
Subjects--Index Terms:

C. elegans

The Role and Regulation of ERK/MAPK in the C. elegans Germline /
LDR:03831nmm a22004213i 4500 001 2432514
005 20250718090821.5
006 m o d
007 cr|nu||||||||
008 261011s2020 miu||||||m |||||||eng d
020 $a 9798557026048
035 $a (MiAaPQD)AAI28262393
035 $a AAI28262393
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Robinson-Thiewes, Sarah, $e author. $3 3821769
245 1 0 $a The Role and Regulation of ERK/MAPK in the C. elegans Germline / $c Sarah Robinson-Thiewes.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 1 electronic resource (155 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 82-07, Section: B.
500 $a Advisors: Kimble, Judith Committee members: Wickens, Marvin; Laughon, Allen; Landick, Robert; Wassarman, David .
502 $b Ph.D. $c The University of Wisconsin - Madison $d 2020.
520 $a Orchestrated gene expression and development are intimately linked. From embryogenesis to tissue homeostasis, cells must control the abundance and timing of gene expression-especially of key developmental factors. An improperly expressed gene may usurp control and drive a cell, tissue, or organism to ruin. Thus, understanding the in vivo role and regulation of critical developmental genes is required. The Caenorhabditis elegans germline is a valuable tool to analyze in vivo germ cell development. This work focuses on ERK/MAPK and the regulatory steps of transcriptional progression. The single C. elegans MAPK ortholog MPK-1 has multiple roles in germline development including sperm/oocyte decision, meiotic progression, and oogenesis. The mpk-1 locus encodes two isoforms, mpk-1a and mpk-1b. The mpk-1b isoform has an exclusive first exon and long first intron; mpk-1a shares all other coding regions with mpk-1b. Each isoform has tissue specific expression: MPK-1B is germline specific and MPK-1A is somatic specific. Despite different tissue localizations, each MPK-1 isoform has a role in germline development. MPK-1B promotes oogenesis and fertility; MPK-1A promotes germline stem cell proliferation and meiotic progression from the soma. Thus, MPK-1 can autonomously and non-autonomously affect germ cell development. Using smFISH (single molecule FISH) targeting either mpk-1b's long first intron or all coding exons, the nascent transcripts at active transcription sites (ATS) and mRNA can be visualized along the germline developmental axis. Surprisingly, the ATS of mpk-1 and Notch components lag-1 and lag-3 exist as either of two classes: the incomplete ATS (iATS) and complete ATS (cATS). The iATS generate incomplete nascent transcripts and cATS generate complete nascent transcripts; therefore, the ATS classes represent different extents of transcriptional progression. Only the cATS frequency correlates with the graded nascent transcripts and mRNA abundance. Furthermore, the iATS and cATS frequencies are graded in a gene-, stage-, and sex-specific manner along the germline. For example, the iATS frequency of mpk-1 and lag-3 is high in the GSC pool whereas lag-1 cATS frequency is high in the same cells. Once cells begin overt differentiation, all three genes have a high cATS frequency. Thus, regulated transcriptional progression is critical for gene expression during development.
546 $a English
590 $a School code: 0262
650 4 $a Genetics. $3 530508
650 4 $a Developmental biology. $3 592588
653 $a C. elegans
653 $a Development
653 $a ERK/MAPK
653 $a Gene expression
653 $a Notch
690 $a 0369
690 $a 0758
710 2 $a The University of Wisconsin - Madison. $b Genetics. $e degree granting institution. $3 3815885
720 1 $a Kimble, Judith $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 82-07B.
790 $a 0262
791 $a Ph.D.
792 $a 2020
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28262393