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Elucidating the Mechanisms of Myosin...

Alazzam, Omayma,

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Elucidating the Mechanisms of Myosin II Force Feedback and Coordination in Actomyosin Ensembles /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Elucidating the Mechanisms of Myosin II Force Feedback and Coordination in Actomyosin Ensembles // Omayma Alazzam.
Author:	Alazzam, Omayma,
Description:	1 electronic resource (314 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-01, Section: B.
Contained By:	Dissertations Abstracts International86-01B.
Subject:	Chemical engineering. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31298251
ISBN:	9798383196205

Elucidating the Mechanisms of Myosin II Force Feedback and Coordination in Actomyosin Ensembles /
Alazzam, Omayma,

Elucidating the Mechanisms of Myosin II Force Feedback and Coordination in Actomyosin Ensembles /Omayma Alazzam. - 1 electronic resource (314 pages)

Source: Dissertations Abstracts International, Volume: 86-01, Section: B.

This dissertation delves into the dynamics of myosin II motors within actomyosin networks, emphasizing their crucial role in muscle functionality and the broader implications for cardiovascular health. Given the critical involvement of myosin II in conditions such as hypertrophic cardiomyopathy (HCM) and heart failure, this research presented here is important for understanding such diseases at the molecular level. Through the use of fluorescence microscopy and optical tweezers, the study reveals novel insights into how myosin II generates force and coordinates within myosin ensembles-a key to grasping the mechanics of muscle contraction. Specifically, this work aimed to unravel the mechanisms of myosin II functionality in actomyosin ensembles and to assess how variations in the dynamics of motor proteins affect force production. We leveraged a novel assay that probes the complex interactions of myosin II within hierarchical structures with mechanical compliancy, allowing for more native-like measurements of force generation and the analysis of myosin ensemble behavior. Results reveal the significant impact of environmental stiffness and the number of engaged motors on the behavior of myosin II ensembles. The study sheds light on a sophisticated system of sensory force feedback among myosin motors, showing that force production results from the collective dynamics of the ensemble, rather than from the actions of individual motors. Additionally, it was discovered that changes in the environmental context and structure of the ensemble can significantly alter force output. This work advances understanding of muscle mechanics at the molecular level and how emergent biophysical properties of the cytoskeleton rely on intricate feedback loops. By elucidating the principles of force generation and coordination within actomyosin networks, this research sets the stage for future studies aimed at understanding the details of the feedback loops, influences of mechanical environments on intracellular crosstalk, and creating targeted treatments for cardiovascular conditions linked to malfunctioning myosin II motors.

English

ISBN: 9798383196205Subjects--Topical Terms:

560457
Chemical engineering.
Subjects--Index Terms:

Actomyosin

Elucidating the Mechanisms of Myosin II Force Feedback and Coordination in Actomyosin Ensembles /
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100 1 $a Alazzam, Omayma, $e author. $3 3821589
245 1 0 $a Elucidating the Mechanisms of Myosin II Force Feedback and Coordination in Actomyosin Ensembles / $c Omayma Alazzam.
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300 $a 1 electronic resource (314 pages)
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500 $a Advisors: Rienemann, Nikki D.; Smith, Adam Committee members: Prager, Brenda; Werfel, Thomas; Walker, Glenn.
502 $b Ph.D. $c The University of Mississippi $d 2024.
520 $a This dissertation delves into the dynamics of myosin II motors within actomyosin networks, emphasizing their crucial role in muscle functionality and the broader implications for cardiovascular health. Given the critical involvement of myosin II in conditions such as hypertrophic cardiomyopathy (HCM) and heart failure, this research presented here is important for understanding such diseases at the molecular level. Through the use of fluorescence microscopy and optical tweezers, the study reveals novel insights into how myosin II generates force and coordinates within myosin ensembles-a key to grasping the mechanics of muscle contraction. Specifically, this work aimed to unravel the mechanisms of myosin II functionality in actomyosin ensembles and to assess how variations in the dynamics of motor proteins affect force production. We leveraged a novel assay that probes the complex interactions of myosin II within hierarchical structures with mechanical compliancy, allowing for more native-like measurements of force generation and the analysis of myosin ensemble behavior. Results reveal the significant impact of environmental stiffness and the number of engaged motors on the behavior of myosin II ensembles. The study sheds light on a sophisticated system of sensory force feedback among myosin motors, showing that force production results from the collective dynamics of the ensemble, rather than from the actions of individual motors. Additionally, it was discovered that changes in the environmental context and structure of the ensemble can significantly alter force output. This work advances understanding of muscle mechanics at the molecular level and how emergent biophysical properties of the cytoskeleton rely on intricate feedback loops. By elucidating the principles of force generation and coordination within actomyosin networks, this research sets the stage for future studies aimed at understanding the details of the feedback loops, influences of mechanical environments on intracellular crosstalk, and creating targeted treatments for cardiovascular conditions linked to malfunctioning myosin II motors.
546 $a English
590 $a School code: 0131
650 4 $a Chemical engineering. $3 560457
650 4 $a Biochemistry. $3 518028
650 4 $a Biomechanics. $3 548685
650 4 $a Biophysics. $3 518360
653 $a Actomyosin
653 $a Hypertrophic cardiomyopathy
653 $a Myosin ensembles
653 $a Muscle contraction
690 $a 0542
690 $a 0786
690 $a 0487
690 $a 0648
710 2 $a The University of Mississippi. $b Chemical Engineering. $e degree granting institution. $3 3821590
720 1 $a Rienemann, Nikki D. $e degree supervisor.
720 1 $a Smith, Adam $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 86-01B.
790 $a 0131
791 $a Ph.D.
792 $a 2024
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31298251