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An Investigation of High-Throughput ...

Supasri, Kanoknate May,

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An Investigation of High-Throughput Proteomic Analysis in Marine Dinoflagellate (Symbiodiniaceae) /

Record Type:	Electronic resources : Monograph/item
Title/Author:	An Investigation of High-Throughput Proteomic Analysis in Marine Dinoflagellate (Symbiodiniaceae) // Kanoknate May Supasri.
Author:	Supasri, Kanoknate May,
Description:	1 electronic resource (180 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
Contained By:	Dissertations Abstracts International85-04B.
Subject:	Nitric oxide. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30612017
ISBN:	9798380498166

An Investigation of High-Throughput Proteomic Analysis in Marine Dinoflagellate (Symbiodiniaceae) /
Supasri, Kanoknate May,

An Investigation of High-Throughput Proteomic Analysis in Marine Dinoflagellate (Symbiodiniaceae) /Kanoknate May Supasri. - 1 electronic resource (180 pages)

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

The marine dinoflagellate called Symbiodinium sp. in the family Symbiodiniaceae has received increasing attention due to its symbiotic relationship with reef-building corals. This symbiosis is sustained through metabolic exchange between the two partners. Stability of this symbiosis rests on metabolic 'compatibility' relative to external resource provisioning (light, nutrients); however, this symbiosis often breaks down ("dysbiosis") under unfavourable environmental perturbations or stress (Suggett & Smith 2020). Despite intensive efforts over several decades to understand the nature of how this symbiosis functions effectively, the underlying regulation mechanisms remain largely unknown.Recently, the increasing availability of genomic and transcriptomic resources for both corals and Symbiodinium has shifted the "molecular horizon" and now provides unprecedented opportunities to investigate invertebrate-microalgal symbiosis. This influx may in turn uncover diagnostic tools for more targeted management of this vulnerable ecosystem. However, relative transcript abundances are not always a good proxy for phenotypic traits, due in part to posttranscriptional/translational regulation and modification. Proteins serve as the ultimate effectors of the variation manifested within genomes and/or transcriptomes, and thus are considered closer to the resulting phenotype. Consequently, proteomics studies may allow for a more realistic picture of the functional cellular response of an organism to environmental stress. However, to date the proteomics insights into cnidarian-microalgal symbiosis has largely stemmed from proteomes of the host invertebrate, and not the microalgal symbiont. Therefore, this thesis applied highthroughput shotgun-based proteomics approaches to investigate and understand the molecular mechanism of light stress tolerance in Symbiodinium.Aim, Objective and hypothesisThe overall aim of the thesis is to investigate the molecular mechanism of light stress tolerance in Symbiodinium using high-throughput proteomics approaches. Additionally, given the fact that peridinin carotenoids have high pharmaceutical activity, a secondary aim of this thesis is to examine the bioactivity of purified peridinin-chlorophyll-protein complex (PCP) protein isolated from Symbiodinium,ultimately to explore their potentials for medicinal applications.The three objectives of the thesis are to:1. Examine the peridinin-chlorophyll-protein complex (PCP) protein accumulation in Symbiodiniumunder light limitation.2. Understand the light stress tolerance behaviour between two Symbiodinium phylotypes; Breviolum sp. (Symbiodinium sp. clade B) andDurusdinium sp. (Symbiodiniumsp. clade D) at the proteomic level.3. Extraction and purification of PCP from Symbiodinium to explore its bioactivity in pharmaceutical applications.

English

ISBN: 9798380498166Subjects--Topical Terms:

750512
Nitric oxide.

An Investigation of High-Throughput Proteomic Analysis in Marine Dinoflagellate (Symbiodiniaceae) /
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245 1 3 $a An Investigation of High-Throughput Proteomic Analysis in Marine Dinoflagellate (Symbiodiniaceae) / $c Kanoknate May Supasri.
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300 $a 1 electronic resource (180 pages)
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500 $a Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
500 $a Advisors: Ralph, Peter; Kumar, Manoj.
502 $b Ph.D. $c University of Technology Sydney (Australia) $d 2021.
520 $a The marine dinoflagellate called Symbiodinium sp. in the family Symbiodiniaceae has received increasing attention due to its symbiotic relationship with reef-building corals. This symbiosis is sustained through metabolic exchange between the two partners. Stability of this symbiosis rests on metabolic 'compatibility' relative to external resource provisioning (light, nutrients); however, this symbiosis often breaks down ("dysbiosis") under unfavourable environmental perturbations or stress (Suggett & Smith 2020). Despite intensive efforts over several decades to understand the nature of how this symbiosis functions effectively, the underlying regulation mechanisms remain largely unknown.Recently, the increasing availability of genomic and transcriptomic resources for both corals and Symbiodinium has shifted the "molecular horizon" and now provides unprecedented opportunities to investigate invertebrate-microalgal symbiosis. This influx may in turn uncover diagnostic tools for more targeted management of this vulnerable ecosystem. However, relative transcript abundances are not always a good proxy for phenotypic traits, due in part to posttranscriptional/translational regulation and modification. Proteins serve as the ultimate effectors of the variation manifested within genomes and/or transcriptomes, and thus are considered closer to the resulting phenotype. Consequently, proteomics studies may allow for a more realistic picture of the functional cellular response of an organism to environmental stress. However, to date the proteomics insights into cnidarian-microalgal symbiosis has largely stemmed from proteomes of the host invertebrate, and not the microalgal symbiont. Therefore, this thesis applied highthroughput shotgun-based proteomics approaches to investigate and understand the molecular mechanism of light stress tolerance in Symbiodinium.Aim, Objective and hypothesisThe overall aim of the thesis is to investigate the molecular mechanism of light stress tolerance in Symbiodinium using high-throughput proteomics approaches. Additionally, given the fact that peridinin carotenoids have high pharmaceutical activity, a secondary aim of this thesis is to examine the bioactivity of purified peridinin-chlorophyll-protein complex (PCP) protein isolated from Symbiodinium,ultimately to explore their potentials for medicinal applications.The three objectives of the thesis are to:1. Examine the peridinin-chlorophyll-protein complex (PCP) protein accumulation in Symbiodiniumunder light limitation.2. Understand the light stress tolerance behaviour between two Symbiodinium phylotypes; Breviolum sp. (Symbiodinium sp. clade B) andDurusdinium sp. (Symbiodiniumsp. clade D) at the proteomic level.3. Extraction and purification of PCP from Symbiodinium to explore its bioactivity in pharmaceutical applications.
546 $a English
590 $a School code: 1295
650 4 $a Nitric oxide. $3 750512
650 4 $a Coral reefs. $3 3564373
650 4 $a Nutrition. $3 517777
650 4 $a Biological oceanography. $3 2122748
690 $a 0570
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710 2 $a University of Technology Sydney (Australia). $e degree granting institution. $3 3814501
720 1 $a Ralph, Peter $e degree supervisor.
720 1 $a Kumar, Manoj $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 85-04B.
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791 $a Ph.D.
792 $a 2021
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30612017