東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Functionalization of a PC-TIR Biosen...

Gonzalez, Christian,

Linked to FindBook

Google Book

Amazon

博客來

Functionalization of a PC-TIR Biosensor for Label-Free cTnI Antigen Detection /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Functionalization of a PC-TIR Biosensor for Label-Free cTnI Antigen Detection // Christian Gonzalez.
Author:	Gonzalez, Christian,
Description:	1 electronic resource (76 pages)
Notes:	Source: Masters Abstracts International, Volume: 84-02.
Contained By:	Masters Abstracts International84-02.
Subject:	Biomedical engineering. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29325628
ISBN:	9798841761051

Functionalization of a PC-TIR Biosensor for Label-Free cTnI Antigen Detection /
Gonzalez, Christian,

Functionalization of a PC-TIR Biosensor for Label-Free cTnI Antigen Detection /Christian Gonzalez. - 1 electronic resource (76 pages)

Source: Masters Abstracts International, Volume: 84-02.

Damaged myocardial cells release specific biomarkers, such as cardiac troponin I (cTnI) antigen, into the bloodstream. Sensitive detection of the cardiac biomarkers is a vital component for early detection of myocardial infarctions and drug-induced cardiotoxicity screenings. In this study, a compact standalone biosensing system was developed based on a Photonic-Crystal structure in a Total-Internal-Reflection (PC-TIR) configuration for label-free detection of cardiac biomarkers. To functionalize the PC-TIR sensor, (3-aminopropyl)triethoxysilane (APTES), carboxymethyl-dextran (CM-dextran), and 3-(triethoxysilyl)propylsuccinic anhydride (TESPSA) were immobilized on the sensing surface. These biochemical pathways were then tested to investigate and compare their binding affinity towards cTnI antibody and, ultimately, their ability to detect cTnI antigen. Additionally, cTnI antigen detection was investigated utilizing UV immobilization of Amino- and PolyT-modified aptamers on an APTES, (3-mercaptopropyl)trimethoxysilane (MPTMS) modified, and unmodified sensor surfaces. The cTnI antibody, Amino-modified aptamer, and Poly-T-modified aptamer running buffer's ionic strength and pH were then optimized to improve the binding affinity between antibodies/aptamers and their respective functionalized surfaces. After proper functionalization with either cTnI antibody or Amino- and Poly-T modified aptamers, the bioassays were evaluated for their respected capabilities to detect for cTnI antigen.

English

ISBN: 9798841761051Subjects--Topical Terms:

535387
Biomedical engineering.
Subjects--Index Terms:

Aptamer

Functionalization of a PC-TIR Biosensor for Label-Free cTnI Antigen Detection /
LDR:02927nmm a22004453i 4500 001 2430612
005 20250527055716.5
006 m o d
007 cr|nu||||||||
008 261011s2022 miu||||||m |||||||eng d
020 $a 9798841761051
035 $a (MiAaPQD)AAI29325628
035 $a AAI29325628
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Gonzalez, Christian, $e author. $0 (orcid)0000-0001-8323-263X $3 3819018
245 1 0 $a Functionalization of a PC-TIR Biosensor for Label-Free cTnI Antigen Detection / $c Christian Gonzalez.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2022
300 $a 1 electronic resource (76 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 84-02.
500 $a Advisors: Ye, JingYong Committee members: Glickman, Randolph; Miyahara, Yoichi; Hood, Robert L.
502 $b M.S. $c The University of Texas at San Antonio $d 2022.
520 $a Damaged myocardial cells release specific biomarkers, such as cardiac troponin I (cTnI) antigen, into the bloodstream. Sensitive detection of the cardiac biomarkers is a vital component for early detection of myocardial infarctions and drug-induced cardiotoxicity screenings. In this study, a compact standalone biosensing system was developed based on a Photonic-Crystal structure in a Total-Internal-Reflection (PC-TIR) configuration for label-free detection of cardiac biomarkers. To functionalize the PC-TIR sensor, (3-aminopropyl)triethoxysilane (APTES), carboxymethyl-dextran (CM-dextran), and 3-(triethoxysilyl)propylsuccinic anhydride (TESPSA) were immobilized on the sensing surface. These biochemical pathways were then tested to investigate and compare their binding affinity towards cTnI antibody and, ultimately, their ability to detect cTnI antigen. Additionally, cTnI antigen detection was investigated utilizing UV immobilization of Amino- and PolyT-modified aptamers on an APTES, (3-mercaptopropyl)trimethoxysilane (MPTMS) modified, and unmodified sensor surfaces. The cTnI antibody, Amino-modified aptamer, and Poly-T-modified aptamer running buffer's ionic strength and pH were then optimized to improve the binding affinity between antibodies/aptamers and their respective functionalized surfaces. After proper functionalization with either cTnI antibody or Amino- and Poly-T modified aptamers, the bioassays were evaluated for their respected capabilities to detect for cTnI antigen.
546 $a English
590 $a School code: 1283
650 4 $a Biomedical engineering. $3 535387
650 4 $a Bioengineering. $3 657580
650 4 $a Biochemistry. $3 518028
653 $a Aptamer
653 $a cTnI antigen
653 $a Functionalization
653 $a PC-TIR
653 $a TESPSA
653 $a UV
690 $a 0541
690 $a 0202
690 $a 0487
710 2 $a The University of Texas at San Antonio. $b Biomedical Engineering. $e degree granting institution. $3 3819019
720 1 $a Ye, JingYong $e degree supervisor.
773 0 $t Masters Abstracts International $g 84-02.
790 $a 1283
791 $a M.S.
792 $a 2022
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29325628