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Computational Model and Sensitivity ...

Profka, Alda,

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Computational Model and Sensitivity Study of Transepithelial/Transendothelial Electrical Resistance for Optimizing in Vitro Models of Cell Barriers /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Computational Model and Sensitivity Study of Transepithelial/Transendothelial Electrical Resistance for Optimizing in Vitro Models of Cell Barriers // Alda Profka.
Author:	Profka, Alda,
Description:	1 electronic resource (105 pages)
Notes:	Source: Masters Abstracts International, Volume: 85-05.
Contained By:	Masters Abstracts International85-05.
Subject:	Physiology. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30718338
ISBN:	9798380704649

Computational Model and Sensitivity Study of Transepithelial/Transendothelial Electrical Resistance for Optimizing in Vitro Models of Cell Barriers /
Profka, Alda,

Computational Model and Sensitivity Study of Transepithelial/Transendothelial Electrical Resistance for Optimizing in Vitro Models of Cell Barriers /Alda Profka. - 1 electronic resource (105 pages)

Source: Masters Abstracts International, Volume: 85-05.

Transepithelial/transendothelial electrical resistance (TEER) is a parameter widely used for monitoring the barrier integrity of an endothelial cell layer in in vitro models. Measuring TEER is a non-invasive and label-free technique that allows for fast and continuous measurements. Although it would be expected to obtain comparable TEER values for the same cell type and conditions, the reported values across the literature vary considerably. To account for differences stemming from specific measurement-related effects, finite element models (FEM) of conventional transwell culture inserts and an in-house microfluidic chip design were created using COMSOL. The microfluidic chip contains four electrodes integrated on each side of the porous membrane without hindering the field of view. It accounts for small changes in temperature or medium composition by deriving the isolated TEER independent of channel properties. The model was experimentally validated by measuring the TEER of a polyester membrane at different electrode positions. The results show the importance of using a geometric correction factor to find the true value of TEER based on the contribution of each zone of the cell layer to cautiously interpret the resulting data. The percentage difference between the simulated and theoretical TEER value represents the error in the measurement. Moreover, the FEM models were used to identify the most and least sensitive geometric and electrical parameters to electrical resistance. By considering the sensitivity distribution and the ranking of the model parameters derived from the numerical simulations, efficient and trustworthy methods can be developed to assess and study the cellular barrier functions. Future microfluidic models containing barrier-forming tissue are expected to be of great value for disease modeling, drug development, and precision medicine. Physiologically-relevant humanoid models can help advance our understanding of pathological conditions such as cancer, several psychiatric and neurodegenerative diseases, and other inflammatory diseases, as well as significantly lower the time and cost of bringing new drugs to the market.

English

ISBN: 9798380704649Subjects--Topical Terms:

518431
Physiology.

Computational Model and Sensitivity Study of Transepithelial/Transendothelial Electrical Resistance for Optimizing in Vitro Models of Cell Barriers /
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245 1 0 $a Computational Model and Sensitivity Study of Transepithelial/Transendothelial Electrical Resistance for Optimizing in Vitro Models of Cell Barriers / $c Alda Profka.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 1 electronic resource (105 pages)
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500 $a Source: Masters Abstracts International, Volume: 85-05.
500 $a Advisors: Mongeau, Luc.
502 $b M.E. $c McGill University (Canada) $d 2023.
520 $a Transepithelial/transendothelial electrical resistance (TEER) is a parameter widely used for monitoring the barrier integrity of an endothelial cell layer in in vitro models. Measuring TEER is a non-invasive and label-free technique that allows for fast and continuous measurements. Although it would be expected to obtain comparable TEER values for the same cell type and conditions, the reported values across the literature vary considerably. To account for differences stemming from specific measurement-related effects, finite element models (FEM) of conventional transwell culture inserts and an in-house microfluidic chip design were created using COMSOL. The microfluidic chip contains four electrodes integrated on each side of the porous membrane without hindering the field of view. It accounts for small changes in temperature or medium composition by deriving the isolated TEER independent of channel properties. The model was experimentally validated by measuring the TEER of a polyester membrane at different electrode positions. The results show the importance of using a geometric correction factor to find the true value of TEER based on the contribution of each zone of the cell layer to cautiously interpret the resulting data. The percentage difference between the simulated and theoretical TEER value represents the error in the measurement. Moreover, the FEM models were used to identify the most and least sensitive geometric and electrical parameters to electrical resistance. By considering the sensitivity distribution and the ranking of the model parameters derived from the numerical simulations, efficient and trustworthy methods can be developed to assess and study the cellular barrier functions. Future microfluidic models containing barrier-forming tissue are expected to be of great value for disease modeling, drug development, and precision medicine. Physiologically-relevant humanoid models can help advance our understanding of pathological conditions such as cancer, several psychiatric and neurodegenerative diseases, and other inflammatory diseases, as well as significantly lower the time and cost of bringing new drugs to the market.
520 $a La resistance electrique transepitheliale /transendotheliale (TEER) est un parametre targement utilise pour surveiller Jintegrite de la barriere d'une couche de cellules endotheliales dans des modeles in vitro. La mesure du TEER est une technique son invasive et sans etiquette qui permet des mesures rapides et continmes. Bien qu'en s'attende a obtenir des valeurs TEER comparables pour le meme type de cellule et les meemes conditions, les valeurs rapportees dans la litterature varient considerablement. Pour tenir compte des differences resultant d'effets specifiques lies a la mesure, des modeles d'elements finis (FEM) des inserts de culture transwell conventionnels et une conception de puce microfluidique interne ont ete crees a Vaide de COMSOL. La puce microfluidique contient quatre electrodes integrees de chaque cote de ta membrane pareuse sans gener le champ de vision. [1 tient compte des petita changements de temperature ou de composition «lu mitieu en derivant le TEER isole independamment des proprietes du canal. Le modele a ete valide experimentalement en mesurant te TEER d'une membrane en polyester a diferentes positions d'electrodes. Nos resultats montrent importance d'utiliser un facteur de correction geometrique pour tronver ta vraie valeur de TEER en fonction de la contributioo de chaque zone de la couche cellulaire pour interpreter avec prudence leu donnee: resultantes. La difference en pourcentage entre la valeur TEER simulee et theorique represente Verreur de mesure. De plus, les modeles FEM ont ete utilises pour identifier les parametres geometriques et electriques les plus et ke moins sensibles a la resistanoe electrique. En considerant la distribution de sensibilite et te classement des parametres du modele derives des simulations aumeriques, des methodes efficaces et fiableu peuvent etre developpees pour evaluer et etudier les fonctions de barriere cellulaire. Les futars modeles microfluidiques contenant des tissus formant une harritre devraient etre d'une grande valeur pour la modelisation des maiadies, le developpement de medicaments et la medecine de precision. S'ils sont appliques avec succes, les modeles humanoides peuvent aider a faire progresser notre comprebension des conditions pathologiques teles que le cancer, plusieurs maladies psychiatriques et neurodegeneratives et d'autres maladies infammatoires, ainsi qu'a raduire considerablement le temps et le cott de mise sur le marche de nouveaux medicaments.
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650 4 $a Membranes. $3 1531702
650 4 $a Tomography. $3 836553
650 4 $a Blood-brain barrier. $3 812171
650 4 $a Electrodes. $3 629151
650 4 $a Spectrum analysis. $3 520440
650 4 $a Semiconductors. $3 516162
650 4 $a Brain research. $3 3561789
650 4 $a Permeability. $3 915594
650 4 $a Design. $3 518875
650 4 $a Nervous system. $3 672432
650 4 $a Ultrasonic imaging. $3 828386
650 4 $a Cell culture. $3 604671
650 4 $a Hyperthermia. $3 3685312
650 4 $a Shear stress. $3 3681838
650 4 $a Analytical chemistry. $3 3168300
650 4 $a Cellular biology. $3 3172791
650 4 $a Medical imaging. $3 3172799
650 4 $a Neurosciences. $3 588700
650 4 $a Optics. $3 517925
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710 2 $a McGill University (Canada). $e degree granting institution. $3 3759190
720 1 $a Mongeau, Luc $e degree supervisor.
773 0 $t Masters Abstracts International $g 85-05.
790 $a 0781
791 $a M.E.
792 $a 2023
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30718338