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Bacteriophage Susceptibility and Gen...

Amarh, Elizabeth D.,

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Bacteriophage Susceptibility and Genomic Analyses of Rapidly Growing and Slowly Growing Clinically Isolated Non-Tuberculous Mycobacteria /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Bacteriophage Susceptibility and Genomic Analyses of Rapidly Growing and Slowly Growing Clinically Isolated Non-Tuberculous Mycobacteria // Elizabeth D Amarh.
作者:	Amarh, Elizabeth D.,
面頁冊數:	1 electronic resource (165 pages)
附註:	Source: Dissertations Abstracts International, Volume: 86-05, Section: B.
Contained By:	Dissertations Abstracts International86-05B.
標題:	Sugar. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31628784
ISBN:	9798346386124

Bacteriophage Susceptibility and Genomic Analyses of Rapidly Growing and Slowly Growing Clinically Isolated Non-Tuberculous Mycobacteria /
Amarh, Elizabeth D.,

Bacteriophage Susceptibility and Genomic Analyses of Rapidly Growing and Slowly Growing Clinically Isolated Non-Tuberculous Mycobacteria /Elizabeth D Amarh. - 1 electronic resource (165 pages)

Source: Dissertations Abstracts International, Volume: 86-05, Section: B.

Non-tuberculous mycobacteria (NTM) cause pulmonary and disseminated infections that are difficult to treat with antibiotics. A renewed interest in phage therapy has yielded positive results in treating antibiotic resistant bacterial infections, including in NTM infections. Mycobacteriophage therapy has been well-documented, with over twenty published cases and zero adverse reactions to treatment. The number of phages that can be applied therapeutically is < 10, and this may be influenced by the host used to isolate most of the over 13,000 mycobacteriophages in the inventory. Most phages are temperate, with the ability to form a prophage in the genome of a susceptible host. The genomes of clinically isolated NTM species are replete with intact prophage sequences. These prophages can be induced to excise from the host genome, re-circularizing the phage genome, and producing phage particles (lytically propagated spontaneously induced prophages (LPSIPs)). We hypothesize these LPSIPs may demonstrate a host range more inclusive of clinically isolated NTM species, as their presence in the NTM genomes suggests they are able to temperately infect clinically isolated NTM successfully. The goal of this research is to increase the repertoire of therapeutically useful phage, by propagating LPSIPs from clinically isolated NTM strains, and generating a lytic mutant of the temperate LPSIP. So far, no LPSIPs isolated from pathogenic NTM infect the non-pathogenic M. smegmatis mc2155. Because usual methods of phage engineering, BRED and CRISPY-BRED, are not available in NTM other than M. smegmatis mc2155, CRISPR-Cas9 instead was implemented. Through characterizing clinically isolated NTM strains phenotypically, assessing the genomes for prophage content, isolating the prophage in LPSIP form, and using CRISPR-Cas9 to engineer a LPSIP lytic mutant, this work expands the repertoire of therapeutically useful mycobacteriophages.

English

ISBN: 9798346386124Subjects--Topical Terms:

3686910
Sugar.

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