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Synthetic Biology and Synchrotron Li...

Flavin, Cory,

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Synthetic Biology and Synchrotron Light Investigations of Emiliania huxleyi Coccolithophores /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Synthetic Biology and Synchrotron Light Investigations of Emiliania huxleyi Coccolithophores // Cory Flavin.
Author:	Flavin, Cory,
Description:	1 electronic resource (139 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
Contained By:	Dissertations Abstracts International86-03B.
Subject:	Biophysics. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31487922
ISBN:	9798384052302

Synthetic Biology and Synchrotron Light Investigations of Emiliania huxleyi Coccolithophores /
Flavin, Cory,

Synthetic Biology and Synchrotron Light Investigations of Emiliania huxleyi Coccolithophores /Cory Flavin. - 1 electronic resource (139 pages)

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

Emiliania huxleyi, the dominant species of coccolithophores in modern oceans, contributes prolific quantities of carbon fixation through calcite biomineralization. This species plays a prominent role in the ocean's biogeochemical cycles and its unique production and shedding of carbonate coccoliths may serve as inspiration for carbon capture and sequestration technologies. This thesis includes three original research studies that set out to discover genes, elements and molecules involved in E. huxleyi biomineralization. The first study employed cell penetrating peptides to overcome the coccosphere barrier and deliver gene specific peptide nucleic acids to viable E. huxleyi cells. Direct delivery of peptide nucleic acids represents a useful synthetic biology tool to further explore coccolithophore biology. In the second study, coccolith elemental incorporation was mapped using high resolution x-ray fluorescence, revealing trace elements embedded in and around the coccolith. Based on elemental maps of sulfur, we hypothesize that sulfur plays and expanded role in the global sulfur cycle and may play a direct role in E. huxleyi biomineralization. In the final study, chemical spectra of single live cells were measured by synchrotron FTIR, demonstrating the capability of differentiating between calcified and decalcified cell types. This works demonstrates a useful result for exploration of E. huxleyi calcification heterogeneity in response to chemical, genetic and environmental perturbations.

English

ISBN: 9798384052302Subjects--Topical Terms:

518360
Biophysics.
Subjects--Index Terms:

Emiliania huxleyi

Synthetic Biology and Synchrotron Light Investigations of Emiliania huxleyi Coccolithophores /
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100 1 $a Flavin, Cory, $e author. $3 3816922
245 1 0 $a Synthetic Biology and Synchrotron Light Investigations of Emiliania huxleyi Coccolithophores / $c Cory Flavin.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2024
300 $a 1 electronic resource (139 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
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500 $a Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
500 $a Advisors: Toney, Michael Committee members: Calve, Sarah; Regueiro, Richard; Hoenger, Andreas; Dowell, Robin.
502 $b Ph.D. $c University of Colorado at Boulder $d 2024.
520 $a Emiliania huxleyi, the dominant species of coccolithophores in modern oceans, contributes prolific quantities of carbon fixation through calcite biomineralization. This species plays a prominent role in the ocean's biogeochemical cycles and its unique production and shedding of carbonate coccoliths may serve as inspiration for carbon capture and sequestration technologies. This thesis includes three original research studies that set out to discover genes, elements and molecules involved in E. huxleyi biomineralization. The first study employed cell penetrating peptides to overcome the coccosphere barrier and deliver gene specific peptide nucleic acids to viable E. huxleyi cells. Direct delivery of peptide nucleic acids represents a useful synthetic biology tool to further explore coccolithophore biology. In the second study, coccolith elemental incorporation was mapped using high resolution x-ray fluorescence, revealing trace elements embedded in and around the coccolith. Based on elemental maps of sulfur, we hypothesize that sulfur plays and expanded role in the global sulfur cycle and may play a direct role in E. huxleyi biomineralization. In the final study, chemical spectra of single live cells were measured by synchrotron FTIR, demonstrating the capability of differentiating between calcified and decalcified cell types. This works demonstrates a useful result for exploration of E. huxleyi calcification heterogeneity in response to chemical, genetic and environmental perturbations.
546 $a English
590 $a School code: 0051
650 4 $a Biophysics. $3 518360
650 4 $a Microbiology. $3 536250
650 4 $a Biogeochemistry. $3 545717
650 4 $a Biological oceanography. $3 2122748
653 $a Emiliania huxleyi
653 $a Carbon fixation
653 $a Carbon capture
653 $a Coccolithophores
653 $a Synthetic biology
690 $a 0786
690 $a 0410
690 $a 0416
690 $a 0425
710 2 $a University of Colorado at Boulder. $b Materials Science and Engineering. $e degree granting institution. $3 3816923
720 1 $a Toney, Michael $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 86-03B.
790 $a 0051
791 $a Ph.D.
792 $a 2024
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31487922