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Assessing Nanoparticle Uptake by Ram...

Scarpitti, Brian Thomas,

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Assessing Nanoparticle Uptake by Raman Microscopy /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Assessing Nanoparticle Uptake by Raman Microscopy // Brian Thomas Scarpitti.
Author:	Scarpitti, Brian Thomas,
Description:	1 electronic resource (138 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-04, Section: B.
Contained By:	Dissertations Abstracts International86-04B.
Subject:	Nanotechnology. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31674155
ISBN:	9798384088707

Assessing Nanoparticle Uptake by Raman Microscopy /
Scarpitti, Brian Thomas,

Assessing Nanoparticle Uptake by Raman Microscopy /Brian Thomas Scarpitti. - 1 electronic resource (138 pages)

Source: Dissertations Abstracts International, Volume: 86-04, Section: B.

This dissertation focuses on the development of Raman microscopy techniques to monitor nanoparticle uptake in cells. Chapter 1 introduces the importance of monitoring nanoparticle delivery for development of new therapeutic and sensing modalities. Nanoparticle characterization methods are then introduced, as these are important in determining nanoparticle properties and utility. Raman microscopy, the primary imaging technique used to monitor particle location in cells, is briefly described. Enhancement of Raman scattering (through either resonance of the laser with an electronic transition of the analyte or analyte adsorption to a plasmonic surface), are also briefly described, as these are critical to their respective applications in nanoparticle imaging.Chapter 2 focuses on Raman monitoring of lycopene-polysorbate 80 nanoparticles in cells. This research shows effective delivery of lycopene into cells. Raman imaging may be used to monitor the quantity, distribution, and isomerization of lycopene in cells. This information contributes to understanding both lycopene delivery to cells and the mechanism of action of lycopene for improving prostate cancer outcomes.Chapters 3 and 4 focus on monitoring gold nanoparticle delivery in cells. Chapter 3 discusses pH sensing methods for determining gold nanoparticle subcellular location. These methods are various data analysis approaches to interpreting the surface enhanced Raman scattering from 4-mercaptobenzoic acid to determine local pH in cells, aiming to infer nanoparticle endosomal escape from pH measurement. The pH method was complicated by the requirement of nanoparticle aggregation for detection, and a limited range of sensitivity of the reporter molecule to pH. Research then focused on spatially localizing every nanoparticle in a cell. Chapter 4 will describe the development of Raman microscopy-based single particle imaging, and a proof of concept in cells. The sensitivity and resolution achieved in this study are required for quantitative subcellular localization of particles, where particles may be densely packed in some subcellular compartments, but sparse in others. These studies work toward determining the efficacy of cell-penetrating peptides in cytosolic delivery of nanoparticles, with potential to advance gold nanoparticle sensing and therapeutic applications.

English

ISBN: 9798384088707Subjects--Topical Terms:

526235
Nanotechnology.
Subjects--Index Terms:

Imaging drug delivery

Assessing Nanoparticle Uptake by Raman Microscopy /
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520 $a This dissertation focuses on the development of Raman microscopy techniques to monitor nanoparticle uptake in cells. Chapter 1 introduces the importance of monitoring nanoparticle delivery for development of new therapeutic and sensing modalities. Nanoparticle characterization methods are then introduced, as these are important in determining nanoparticle properties and utility. Raman microscopy, the primary imaging technique used to monitor particle location in cells, is briefly described. Enhancement of Raman scattering (through either resonance of the laser with an electronic transition of the analyte or analyte adsorption to a plasmonic surface), are also briefly described, as these are critical to their respective applications in nanoparticle imaging.Chapter 2 focuses on Raman monitoring of lycopene-polysorbate 80 nanoparticles in cells. This research shows effective delivery of lycopene into cells. Raman imaging may be used to monitor the quantity, distribution, and isomerization of lycopene in cells. This information contributes to understanding both lycopene delivery to cells and the mechanism of action of lycopene for improving prostate cancer outcomes.Chapters 3 and 4 focus on monitoring gold nanoparticle delivery in cells. Chapter 3 discusses pH sensing methods for determining gold nanoparticle subcellular location. These methods are various data analysis approaches to interpreting the surface enhanced Raman scattering from 4-mercaptobenzoic acid to determine local pH in cells, aiming to infer nanoparticle endosomal escape from pH measurement. The pH method was complicated by the requirement of nanoparticle aggregation for detection, and a limited range of sensitivity of the reporter molecule to pH. Research then focused on spatially localizing every nanoparticle in a cell. Chapter 4 will describe the development of Raman microscopy-based single particle imaging, and a proof of concept in cells. The sensitivity and resolution achieved in this study are required for quantitative subcellular localization of particles, where particles may be densely packed in some subcellular compartments, but sparse in others. These studies work toward determining the efficacy of cell-penetrating peptides in cytosolic delivery of nanoparticles, with potential to advance gold nanoparticle sensing and therapeutic applications.
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650 4 $a Biochemistry. $3 518028
650 4 $a Nanoscience. $3 587832
650 4 $a Analytical chemistry. $3 3168300
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653 $a Raman microscopy
653 $a Nanoparticles
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710 2 $a The Ohio State University. $b Biochemistry Program, Ohio State. $e degree granting institution. $3 3816521
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