東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Digital Microfluidic Oligonucleotide...

Houchaimi, Sari,

Linked to FindBook

Google Book

Amazon

博客來

Digital Microfluidic Oligonucleotide Synthesis: Automated Assembly of DNA Products in Air and Oil With Integrated On-Chip Incubation /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Digital Microfluidic Oligonucleotide Synthesis: Automated Assembly of DNA Products in Air and Oil With Integrated On-Chip Incubation // Sari Houchaimi.
Author:	Houchaimi, Sari,
Description:	1 electronic resource (96 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-06, Section: B.
Contained By:	Dissertations Abstracts International86-06B.
Subject:	Engineering. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31635416
ISBN:	9798346752288

Digital Microfluidic Oligonucleotide Synthesis: Automated Assembly of DNA Products in Air and Oil With Integrated On-Chip Incubation /
Houchaimi, Sari,

Digital Microfluidic Oligonucleotide Synthesis: Automated Assembly of DNA Products in Air and Oil With Integrated On-Chip Incubation /Sari Houchaimi. - 1 electronic resource (96 pages)

Source: Dissertations Abstracts International, Volume: 86-06, Section: B.

Production of accurate DNA products is essential for many biological applications including drug development and delivery, synthetic biology, molecular engineering and epigenetics. It is difficult to build long DNA chains accurately and repeatably with conventional methods such as phosphoramidite method and polymerase chain assembly. DNA aqueous enzymatic assembly is a promising alternative. Multiple DNA fragments can be reliably and consistently assembled into one product using engineering enzymes in an aqueous medium. Digital Microfluidics uses asymmetric electric fields to simultaneously manipulate multiple reagent droplets on an array of electrodes. This platform is biocompatible and easily controllable. It has been used for point-of-care testing and biological assay protocols while increasing throughput and minimizing reagent waste. It is a prime candidate for performing DNA assembly.This work successfully demonstrates assembly of single and double-stranded oligonucleotides on digital microfluidic devices in air and oil. Two DNA bricks were assembled in air and in oil to form a product 250 base pairs long. Each brick consisted of three annealed 80-nucleotide long oligonucleotides. Three, four and five single-stranded oligonucleotides (each between 28 and 50 nucleotides long) were assembled in air to obtain products of 125, 150, and 175 base pairs long respectively. On-chip incubations were performed at room temperature and 37°C by integrating an external heater into a commercially available DMF control system. In these experiments, droplets were surrounded by an oil medium to eliminate evaporation. DNA products were compared to benchtop controls to verify their success.

English

ISBN: 9798346752288Subjects--Topical Terms:

586835
Engineering.
Subjects--Index Terms:

Assembly

Digital Microfluidic Oligonucleotide Synthesis: Automated Assembly of DNA Products in Air and Oil With Integrated On-Chip Incubation /
LDR:03273nmm a22004453i 4500 001 2428628
005 20250414021917.5
006 m o d
007 cr|nu||||||||
008 261011s2024 miu||||||m |||||||eng d
020 $a 9798346752288
035 $a (MiAaPQD)AAI31635416
035 $a AAI31635416
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Houchaimi, Sari, $e author. $3 3815970
245 1 0 $a Digital Microfluidic Oligonucleotide Synthesis: Automated Assembly of DNA Products in Air and Oil With Integrated On-Chip Incubation / $c Sari Houchaimi.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2024
300 $a 1 electronic resource (96 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 86-06, Section: B.
500 $a Advisors: Schertzer, Michael J. Committee members: Iglesias Victoria, Patricia; Lamkin-Kennard, Kathleen; Michel, Lea; Schrlau, Michael.
502 $b Ph.D. $c Rochester Institute of Technology $d 2024.
520 $a Production of accurate DNA products is essential for many biological applications including drug development and delivery, synthetic biology, molecular engineering and epigenetics. It is difficult to build long DNA chains accurately and repeatably with conventional methods such as phosphoramidite method and polymerase chain assembly. DNA aqueous enzymatic assembly is a promising alternative. Multiple DNA fragments can be reliably and consistently assembled into one product using engineering enzymes in an aqueous medium. Digital Microfluidics uses asymmetric electric fields to simultaneously manipulate multiple reagent droplets on an array of electrodes. This platform is biocompatible and easily controllable. It has been used for point-of-care testing and biological assay protocols while increasing throughput and minimizing reagent waste. It is a prime candidate for performing DNA assembly.This work successfully demonstrates assembly of single and double-stranded oligonucleotides on digital microfluidic devices in air and oil. Two DNA bricks were assembled in air and in oil to form a product 250 base pairs long. Each brick consisted of three annealed 80-nucleotide long oligonucleotides. Three, four and five single-stranded oligonucleotides (each between 28 and 50 nucleotides long) were assembled in air to obtain products of 125, 150, and 175 base pairs long respectively. On-chip incubations were performed at room temperature and 37°C by integrating an external heater into a commercially available DMF control system. In these experiments, droplets were surrounded by an oil medium to eliminate evaporation. DNA products were compared to benchtop controls to verify their success.
546 $a English
590 $a School code: 0465
650 4 $a Engineering. $3 586835
650 4 $a Materials science. $3 543314
650 4 $a Fluid mechanics. $3 528155
650 4 $a Mechanical engineering. $3 649730
653 $a Assembly
653 $a Digital microfluidics
653 $a Heater
653 $a Oligonucleotides
653 $a On-chip incubation
690 $a 0537
690 $a 0794
690 $a 0548
690 $a 0204
710 2 $a Rochester Institute of Technology. $b Engineering. $e degree granting institution. $3 3815971
720 1 $a Schertzer, Michael J. $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 86-06B.
790 $a 0465
791 $a Ph.D.
792 $a 2024
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31635416