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Mechanisms of Nerve Dependence and I...

Osorio-Mendez, Daniel,

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Mechanisms of Nerve Dependence and Innervation in Zebrafish Caudal Fin Regeneration /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Mechanisms of Nerve Dependence and Innervation in Zebrafish Caudal Fin Regeneration // Daniel Osorio-Mendez.
作者:	Osorio-Mendez, Daniel,
面頁冊數:	1 electronic resource (173 pages)
附註:	Source: Dissertations Abstracts International, Volume: 86-01, Section: B.
Contained By:	Dissertations Abstracts International86-01B.
標題:	Developmental biology. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31335609
ISBN:	9798383197653

Mechanisms of Nerve Dependence and Innervation in Zebrafish Caudal Fin Regeneration /
Osorio-Mendez, Daniel,

Mechanisms of Nerve Dependence and Innervation in Zebrafish Caudal Fin Regeneration /Daniel Osorio-Mendez. - 1 electronic resource (173 pages)

Source: Dissertations Abstracts International, Volume: 86-01, Section: B.

The capacity to regenerate appendages is not well conserved among species. Whereas teleost fishes and urodele amphibians can regenerate amputated appendages, this ability is restricted to digit tips in adult mammals. One key element shared among regenerative species is that regeneration requires a nerve supply, a phenomenon termed nerve dependence. Despite nerve dependence being first reported in salamander limbs in the early 1820s, many questions remain regarding the identity of the neuronal factors mediating regeneration and the mechanisms of peripheral nerve growth after appendage amputation. In this thesis, we use the adult zebrafish caudal fin to identify novel regulators of fin regeneration and investigate nerve dependence in the context of reinnervation of regenerating fins. In Chapter I, we uncover the voltage-gated sodium channel (VGSC) scn8ab (Nav1.6) as a novel neuronal regulator of zebrafish fin regeneration. Through a forward genetics screen, we identified a striking mutant, temperature-triggered cataplexy (temca), exhibiting fin regeneration and locomotion defects resulting from a missense mutation in scn8ab. We find that scn8ab is essential for regeneration by promoting reinnervation of regenerating fins. In Chapter II, we explore the cells and signals from the regenerate that mediate fin reinnervation after amputation. We show that caudal fin reinnervation is dynamic, with nerves broadly innervating the blastema as it forms, but migrating to its periphery at later time points, unexpectedly leaving the blastema center devoid of axons. We then find that osteoblast progenitors in the periphery of the blastema mediate nerve regrowth in regenerating caudal fins, likely through SemaphorinPlexin signaling. Taken together, this work highlights the impact of peripheral nerve growth in the context of appendage regeneration and the significance of understanding this process for devising novel strategies for the treatment of injured tissues.

English

ISBN: 9798383197653Subjects--Topical Terms:

592588
Developmental biology.
Subjects--Index Terms:

Fin regeneration

Mechanisms of Nerve Dependence and Innervation in Zebrafish Caudal Fin Regeneration /
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520 $a The capacity to regenerate appendages is not well conserved among species. Whereas teleost fishes and urodele amphibians can regenerate amputated appendages, this ability is restricted to digit tips in adult mammals. One key element shared among regenerative species is that regeneration requires a nerve supply, a phenomenon termed nerve dependence. Despite nerve dependence being first reported in salamander limbs in the early 1820s, many questions remain regarding the identity of the neuronal factors mediating regeneration and the mechanisms of peripheral nerve growth after appendage amputation. In this thesis, we use the adult zebrafish caudal fin to identify novel regulators of fin regeneration and investigate nerve dependence in the context of reinnervation of regenerating fins. In Chapter I, we uncover the voltage-gated sodium channel (VGSC) scn8ab (Nav1.6) as a novel neuronal regulator of zebrafish fin regeneration. Through a forward genetics screen, we identified a striking mutant, temperature-triggered cataplexy (temca), exhibiting fin regeneration and locomotion defects resulting from a missense mutation in scn8ab. We find that scn8ab is essential for regeneration by promoting reinnervation of regenerating fins. In Chapter II, we explore the cells and signals from the regenerate that mediate fin reinnervation after amputation. We show that caudal fin reinnervation is dynamic, with nerves broadly innervating the blastema as it forms, but migrating to its periphery at later time points, unexpectedly leaving the blastema center devoid of axons. We then find that osteoblast progenitors in the periphery of the blastema mediate nerve regrowth in regenerating caudal fins, likely through SemaphorinPlexin signaling. Taken together, this work highlights the impact of peripheral nerve growth in the context of appendage regeneration and the significance of understanding this process for devising novel strategies for the treatment of injured tissues.
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