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Investigating the KAI2-Mediated Sign...

Stirling, Shannon,

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Investigating the KAI2-Mediated Signaling Pathway of Volatile Sesquiterpenes /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Investigating the KAI2-Mediated Signaling Pathway of Volatile Sesquiterpenes // Shannon Stirling.
Author:	Stirling, Shannon,
Description:	1 electronic resource (129 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 86-04, Section: B.
Contained By:	Dissertations Abstracts International86-04B.
Subject:	Volatile organic compounds--VOCs. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31285080
ISBN:	9798342101516

Investigating the KAI2-Mediated Signaling Pathway of Volatile Sesquiterpenes /
Stirling, Shannon,

Investigating the KAI2-Mediated Signaling Pathway of Volatile Sesquiterpenes /Shannon Stirling. - 1 electronic resource (129 pages)

Source: Dissertations Abstracts International, Volume: 86-04, Section: B.

Plants emit an amazing diversity of volatile organic compounds (VOCs) that in addition to being utilized by humans for a multitude of applications, allow plants to communicate with their environment, and play numerous roles in plant growth and development. Plants must be able to perceive and distinguish between VOC cues mediating plant-plant, plant-insect, and plant-microbe interactions to appropriately respond to stimuli. Due to the plethora of biological processes dependent on VOCs, significant progress has been made towards understanding the biosynthesis of plant VOCs and their regulation, and, in recent years, the molecular mechanisms involved in VOC emission. However, to date, little is known about how VOCs are perceived by plants and trigger cellular response(s). In animals, VOCs are recognized by odorant receptors known as G-protein-coupled receptor (GPCR) proteins. However, the few GPCR genes identified in plants appear to have different functions and the lack of a reliable marker for VOC perception has hampered research in this field.The discovery of natural fumigation of terpenoids in petunias provides a means of studying VOC perception and the downstream signaling pathways by providing a visual indicator of perception. Transcriptomic analysis of wild-type and transgenic petunias deficient in terpenoid synthesis revealed a link between terpene perception in pistils with the karrikin-like signaling pathway. By utilizing biochemical, computational, and in planta experiments, we demonstrate that of the four petunia karrikin-insensitive receptors (PhKAI2), one of the Lamiid-specific KAI2 intermediate clade receptors, PhKAI2ia, can stereo-specifically perceive the (−)-germacrene D signal emitted from the floral tubes, triggering a KAI2-mediated signaling cascade and affecting plant fitness. Downregulation of PhKAI2ia results in significantly smaller stigmas compared to wild-type, and the phenotype cannot be complemented by the treatment of pistils with (−)-germacrene D, indicating that PhKAI2ia transgenic plants are acting as deaf receptors. We also show that the binding of (−)-germacrene D to PhKAI2ia is sufficient to induce complex formation with more axillary growth 2 (PhMAX2) and the subsequent degradation of suppressor of MAX2 (PhSMAX1a).Altogether, our research uncovers the role(s) of the intermediate clade of KAI2 receptors, illuminates the involvement of a KAI2ia-dependent signaling pathway in volatile communication, and provides new insights into plant olfaction and the long-standing question about the nature of potential endogenous KAI2 ligand(s).

English

ISBN: 9798342101516Subjects--Topical Terms:

3683745
Volatile organic compounds--VOCs.

Investigating the KAI2-Mediated Signaling Pathway of Volatile Sesquiterpenes /
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520 $a Plants emit an amazing diversity of volatile organic compounds (VOCs) that in addition to being utilized by humans for a multitude of applications, allow plants to communicate with their environment, and play numerous roles in plant growth and development. Plants must be able to perceive and distinguish between VOC cues mediating plant-plant, plant-insect, and plant-microbe interactions to appropriately respond to stimuli. Due to the plethora of biological processes dependent on VOCs, significant progress has been made towards understanding the biosynthesis of plant VOCs and their regulation, and, in recent years, the molecular mechanisms involved in VOC emission. However, to date, little is known about how VOCs are perceived by plants and trigger cellular response(s). In animals, VOCs are recognized by odorant receptors known as G-protein-coupled receptor (GPCR) proteins. However, the few GPCR genes identified in plants appear to have different functions and the lack of a reliable marker for VOC perception has hampered research in this field.The discovery of natural fumigation of terpenoids in petunias provides a means of studying VOC perception and the downstream signaling pathways by providing a visual indicator of perception. Transcriptomic analysis of wild-type and transgenic petunias deficient in terpenoid synthesis revealed a link between terpene perception in pistils with the karrikin-like signaling pathway. By utilizing biochemical, computational, and in planta experiments, we demonstrate that of the four petunia karrikin-insensitive receptors (PhKAI2), one of the Lamiid-specific KAI2 intermediate clade receptors, PhKAI2ia, can stereo-specifically perceive the (−)-germacrene D signal emitted from the floral tubes, triggering a KAI2-mediated signaling cascade and affecting plant fitness. Downregulation of PhKAI2ia results in significantly smaller stigmas compared to wild-type, and the phenotype cannot be complemented by the treatment of pistils with (−)-germacrene D, indicating that PhKAI2ia transgenic plants are acting as deaf receptors. We also show that the binding of (−)-germacrene D to PhKAI2ia is sufficient to induce complex formation with more axillary growth 2 (PhMAX2) and the subsequent degradation of suppressor of MAX2 (PhSMAX1a).Altogether, our research uncovers the role(s) of the intermediate clade of KAI2 receptors, illuminates the involvement of a KAI2ia-dependent signaling pathway in volatile communication, and provides new insights into plant olfaction and the long-standing question about the nature of potential endogenous KAI2 ligand(s).
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650 4 $a Flowers & plants. $3 3564028
650 4 $a Success. $3 518195
650 4 $a Butterflies & moths. $3 3692212
650 4 $a Communication. $3 524709
650 4 $a Plastids. $3 2106601
650 4 $a Biosynthesis. $3 516407
650 4 $a Amino acids. $3 558768
650 4 $a Pollinators. $3 3241649
650 4 $a Transgenic plants. $3 525755
650 4 $a Oils & fats. $3 3561739
650 4 $a Membranes. $3 1531702
650 4 $a Signal transduction. $3 3546420
650 4 $a Herbivores. $3 1899108
650 4 $a Seeds. $3 573605
650 4 $a Insects. $3 516323
650 4 $a Animal reproduction. $3 3680772
650 4 $a Plant reproduction. $3 3683497
650 4 $a Agronomy. $3 2122783
650 4 $a Animal sciences. $3 3174829
650 4 $a Botany. $3 516217
650 4 $a Entomology. $3 615844
650 4 $a Organic chemistry. $3 523952
650 4 $a Plant sciences. $3 3173832
650 4 $a Toxicology. $3 556884
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