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Effects of Acetylsalicylic Acid on O...

Khampatee, Vissuta.

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Effects of Acetylsalicylic Acid on Odontogenesis of Human Dental Pulp Cells and TGF-s1 Liberation from Dentin.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Effects of Acetylsalicylic Acid on Odontogenesis of Human Dental Pulp Cells and TGF-s1 Liberation from Dentin./
作者:	Khampatee, Vissuta.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2024,
面頁冊數:	211 p.
附註:	Source: Dissertations Abstracts International, Volume: 85-01, Section: B.
Contained By:	Dissertations Abstracts International85-01B.
標題:	Materials science. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30566410
ISBN:	9798379899790

Effects of Acetylsalicylic Acid on Odontogenesis of Human Dental Pulp Cells and TGF-s1 Liberation from Dentin.
Khampatee, Vissuta.

Effects of Acetylsalicylic Acid on Odontogenesis of Human Dental Pulp Cells and TGF-s1 Liberation from Dentin. - Ann Arbor : ProQuest Dissertations & Theses, 2024 - 211 p.

Source: Dissertations Abstracts International, Volume: 85-01, Section: B.

Thesis (D.Sc.D.)--Boston University, 2024.

Acetylsalicylic acid (ASA), aspirin, is a renowned NSAID that its role in the process of bone metabolism has recently come to light. However, the influence of ASA on the odontogenesis of human dental pulp cells (HDPCs) remains elusive. In search of materials that would synergize the healing potential of the dental pulp, this study aimed to investigate the role of ASA on the odontogenesis of HDPCs in vitro and the influence of ASA on TGF-s1 liberation from dentin.HDPCs were cultured in a culture medium with different concentrations of ASA: 25, 50, 75, 100, 200 g/mL and 0 g/mL as a control. The mitochondria activity of HDPCs was assessed using an MTT assay. Crystal violet staining and triton were used to evaluate cell proliferation rates. ALP activity was measured with the fluorometric assay. Expressions of DSP and RUNX2 were determined with ELISA. DSP and RUNX2 mRNA levels were measured with RT‐qPCR. Alizarin red staining was conducted to evaluate the mineralized nodule formation. Dentin slices were submerged in PBS (negative control), 17% EDTA (positive control), and ASA before collecting the solution for TGF-s1viquantification by ELISA. The data were analyzed by t tests and ANOVA followed by the Tukey post hoc tests. P values < 0.05 were considered statistically significant.The results showed that 25-50 µg/mL ASA promoted mitochondria activity of HDPCs at 72h (P<0.05) and yielded significantly higher proliferation rates of HDPCs than the control at 14d and 21d (P<0.001). All concentrations of ASA promoted odontogenic differentiation of HDPCs by enhancing the mineralization and the levels of DSP, RUNX2, and their mRNA expression in a dose-dependent manner (P<0.05). Also, ASA yielded significantly higher TGF-s1 liberation after conditioning dentin for 5min (P<0.001) and 10min (P<0.05).In conclusion, the data suggest that ASA promotes the odontogenic potential of HDPCs and TGF-s1 liberation from dentin in vitro and might be incorporated into the novel pulp capping materials for dental tissue regeneration.

ISBN: 9798379899790Subjects--Topical Terms:

543314
Materials science.
Subjects--Index Terms:

Acetylsalicylic acid

Effects of Acetylsalicylic Acid on Odontogenesis of Human Dental Pulp Cells and TGF-s1 Liberation from Dentin.
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520 $a Acetylsalicylic acid (ASA), aspirin, is a renowned NSAID that its role in the process of bone metabolism has recently come to light. However, the influence of ASA on the odontogenesis of human dental pulp cells (HDPCs) remains elusive. In search of materials that would synergize the healing potential of the dental pulp, this study aimed to investigate the role of ASA on the odontogenesis of HDPCs in vitro and the influence of ASA on TGF-s1 liberation from dentin.HDPCs were cultured in a culture medium with different concentrations of ASA: 25, 50, 75, 100, 200 g/mL and 0 g/mL as a control. The mitochondria activity of HDPCs was assessed using an MTT assay. Crystal violet staining and triton were used to evaluate cell proliferation rates. ALP activity was measured with the fluorometric assay. Expressions of DSP and RUNX2 were determined with ELISA. DSP and RUNX2 mRNA levels were measured with RT‐qPCR. Alizarin red staining was conducted to evaluate the mineralized nodule formation. Dentin slices were submerged in PBS (negative control), 17% EDTA (positive control), and ASA before collecting the solution for TGF-s1viquantification by ELISA. The data were analyzed by t tests and ANOVA followed by the Tukey post hoc tests. P values < 0.05 were considered statistically significant.The results showed that 25-50 µg/mL ASA promoted mitochondria activity of HDPCs at 72h (P<0.05) and yielded significantly higher proliferation rates of HDPCs than the control at 14d and 21d (P<0.001). All concentrations of ASA promoted odontogenic differentiation of HDPCs by enhancing the mineralization and the levels of DSP, RUNX2, and their mRNA expression in a dose-dependent manner (P<0.05). Also, ASA yielded significantly higher TGF-s1 liberation after conditioning dentin for 5min (P<0.001) and 10min (P<0.05).In conclusion, the data suggest that ASA promotes the odontogenic potential of HDPCs and TGF-s1 liberation from dentin in vitro and might be incorporated into the novel pulp capping materials for dental tissue regeneration.
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653 $a Cell differentiation
653 $a Dental pulp capping
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653 $a Regenerative medicine
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