東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

The Dentinogenic Effects of Magnesiu...

Salem, Rania.

FindBook

Google Book

Amazon

博客來

The Dentinogenic Effects of Magnesium Chloride and Magnesium Oxide on Human Dental Pulp Cells: An in vitro Study.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The Dentinogenic Effects of Magnesium Chloride and Magnesium Oxide on Human Dental Pulp Cells: An in vitro Study./
作者:	Salem, Rania.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2024,
面頁冊數:	696 p.
附註:	Source: Dissertations Abstracts International, Volume: 84-07, Section: B.
Contained By:	Dissertations Abstracts International84-07B.
標題:	Dentistry. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30242746
ISBN:	9798368425368

The Dentinogenic Effects of Magnesium Chloride and Magnesium Oxide on Human Dental Pulp Cells: An in vitro Study.
Salem, Rania.

The Dentinogenic Effects of Magnesium Chloride and Magnesium Oxide on Human Dental Pulp Cells: An in vitro Study. - Ann Arbor : ProQuest Dissertations & Theses, 2024 - 696 p.

Source: Dissertations Abstracts International, Volume: 84-07, Section: B.

Thesis (D.Sc.D.)--Boston University, 2024.

Background: Magnesium-based biomaterials might provide an innovative therapeutic potential to substantially enhance regeneration of dental tissues. Magnesium (Mg2+) has been considered for its potential ability to accelerate proliferation and differentiation of human osteoblasts. However, to date, the dentinogenic effect of magnesium chloride (MgCl2) and magnesium oxide (MgO) on human dental pulp cells (HDPCs) has not been investigated. Purpose: This study was designed to compare the stimulatory effect of different concentrations of MgCl2 and MgO on dentinogenesis of HDPCs and to explore associated cellular signaling pathways. Methods: HDPCs were cultured with 0.5 mM,1 mM, 2 mM, 4 mM, 8 mM concentrations of supplemental MgCl2 and MgO, 0 mM as negative control group, lignin sulfonic acid sodium salt and xanthan gum as vehicle control groups. Crystal violet staining was used to determine cell attachment and proliferation rate. Cell viability was investigated by MTT assay. Odontogenic differentiation was assessed by evaluating alkaline phosphatase (ALP) activity, expression of dentin sialoprotein (DSP), dentin matrix protein1(DMP-1), dentin sialophosphoprotein (DSPP), type I collagen (COL-I), and mineralization. Expression of bone morphogenic protein (BMP-2), phosphorylated SMADs 1/5/9, p-p38, p38, p-JNK, JNK, p-ERK1/2, ERK1/2 mitogen activated protein kinase (MAPK) were also investigated. Statistical analysis was applied using multi-way ANOVA with Wilks' lambda test. Results: 0.5 mM-2 mM MgCl2 elicited the highest stimulatory effect on attachment, proliferation rate, ALP activity, expression of dentinogenic proteins (DSP, DMP-1, DSPP, COL-I), expression of cellular signaling proteins (BMP-2, phosphorylated SMADs1/5/9, p-p38, p-JNK) mineralization, and down regulation of p-ERK 1/2 compared to negative control (P < 0.0001). 0.5 mM supplemental MgO showed higher attachment, proliferation, cell viability, ALP activity, expression of dentinogenic proteins (DSP, DMP-1, DSPP, COL-I), cellular signaling proteins (BMP-2, phosphorylated SMADs1/5/9) and mineralization, compared to negative control (P < 0.001). Conclusion: This study demonstrated the significant benefit imparted by optimum concentrations of MgCl2 and MgO on HDPCs evidenced by upregulated cell attachment, proliferation, cell viability, ALP activity, mineralization, expression of odontogenic proteins and cellular signaling proteins. Compared with MgO, MgCl2 yielded a wider range of effective concentrations (0.5 mM-2 mM MgCl2 vs. 0.5 mM MgO) for upregulating the dentinogenic effect of HDPCs. MgCl2 at optimal concentrations could be a potential novel material for dentin repair in regenerative endodontics.

ISBN: 9798368425368Subjects--Topical Terms:

828971
Dentistry.
Subjects--Index Terms:

Magnesium-based biomaterials

The Dentinogenic Effects of Magnesium Chloride and Magnesium Oxide on Human Dental Pulp Cells: An in vitro Study.
LDR:03728nmm a2200313 4500 001 2404311
005 20241209114557.5
006 m o d
007 cr#unu||||||||
008 251215s2024 ||||||||||||||||| ||eng d
020 $a 9798368425368
035 $a (MiAaPQ)AAI30242746
035 $a AAI30242746
040 $a MiAaPQ $c MiAaPQ
100 1 $a Salem, Rania. $0 (orcid)0000-0002-3614-0035 $3 3774619
245 1 0 $a The Dentinogenic Effects of Magnesium Chloride and Magnesium Oxide on Human Dental Pulp Cells: An in vitro Study.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2024
300 $a 696 p.
500 $a Source: Dissertations Abstracts International, Volume: 84-07, Section: B.
500 $a Advisor: Chou, Lasheing.
502 $a Thesis (D.Sc.D.)--Boston University, 2024.
520 $a Background: Magnesium-based biomaterials might provide an innovative therapeutic potential to substantially enhance regeneration of dental tissues. Magnesium (Mg2+) has been considered for its potential ability to accelerate proliferation and differentiation of human osteoblasts. However, to date, the dentinogenic effect of magnesium chloride (MgCl2) and magnesium oxide (MgO) on human dental pulp cells (HDPCs) has not been investigated. Purpose: This study was designed to compare the stimulatory effect of different concentrations of MgCl2 and MgO on dentinogenesis of HDPCs and to explore associated cellular signaling pathways. Methods: HDPCs were cultured with 0.5 mM,1 mM, 2 mM, 4 mM, 8 mM concentrations of supplemental MgCl2 and MgO, 0 mM as negative control group, lignin sulfonic acid sodium salt and xanthan gum as vehicle control groups. Crystal violet staining was used to determine cell attachment and proliferation rate. Cell viability was investigated by MTT assay. Odontogenic differentiation was assessed by evaluating alkaline phosphatase (ALP) activity, expression of dentin sialoprotein (DSP), dentin matrix protein1(DMP-1), dentin sialophosphoprotein (DSPP), type I collagen (COL-I), and mineralization. Expression of bone morphogenic protein (BMP-2), phosphorylated SMADs 1/5/9, p-p38, p38, p-JNK, JNK, p-ERK1/2, ERK1/2 mitogen activated protein kinase (MAPK) were also investigated. Statistical analysis was applied using multi-way ANOVA with Wilks' lambda test. Results: 0.5 mM-2 mM MgCl2 elicited the highest stimulatory effect on attachment, proliferation rate, ALP activity, expression of dentinogenic proteins (DSP, DMP-1, DSPP, COL-I), expression of cellular signaling proteins (BMP-2, phosphorylated SMADs1/5/9, p-p38, p-JNK) mineralization, and down regulation of p-ERK 1/2 compared to negative control (P < 0.0001). 0.5 mM supplemental MgO showed higher attachment, proliferation, cell viability, ALP activity, expression of dentinogenic proteins (DSP, DMP-1, DSPP, COL-I), cellular signaling proteins (BMP-2, phosphorylated SMADs1/5/9) and mineralization, compared to negative control (P < 0.001). Conclusion: This study demonstrated the significant benefit imparted by optimum concentrations of MgCl2 and MgO on HDPCs evidenced by upregulated cell attachment, proliferation, cell viability, ALP activity, mineralization, expression of odontogenic proteins and cellular signaling proteins. Compared with MgO, MgCl2 yielded a wider range of effective concentrations (0.5 mM-2 mM MgCl2 vs. 0.5 mM MgO) for upregulating the dentinogenic effect of HDPCs. MgCl2 at optimal concentrations could be a potential novel material for dentin repair in regenerative endodontics.
590 $a School code: 0017.
650 4 $a Dentistry. $3 828971
653 $a Magnesium-based biomaterials
690 $a 0567
710 2 $a Boston University. $b Restorative Sciences & Biomaterials GSDM. $3 3691633
773 0 $t Dissertations Abstracts International $g 84-07B.
790 $a 0017
791 $a D.Sc.D.
792 $a 2024
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30242746