東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Characterizing Memory Trace Reactiva...

Wang, Lijing.

Linked to FindBook

Google Book

Amazon

博客來

Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus./
Author:	Wang, Lijing.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
Description:	122 p.
Notes:	Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
Contained By:	Dissertations Abstracts International85-03B.
Subject:	Neurosciences. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30748423
ISBN:	9798380374408

Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus.
Wang, Lijing.

Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 122 p.

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

Thesis (Ph.D.)--University of Michigan, 2023.

Over a century of research in both animal models and human subjects shows that sleep may improve, while sleep loss may disrupt, memory task performance. One prevailing hypothesis, active systems consolidation (ASC), posits that during sleep, neurons encoding newly acquired memories are reactivated, and the connections within the network get strengthened, resulting in the formation of a more persistent long-term memory. My thesis tests this hypothesis by quantifying the reactivation of memory traces in the mouse hippocampus during post-learning sleep. I also characterize learning- and sleep-induced biosynthetic changes in different subregions of the hippocampus to clarify the molecular mechanisms associated with this reactivation, in the context of sleep-dependent contextual fear memory consolidation.Using the targeted recombination in active populations (TRAP) mouse line, I quantified reactivation of hippocampal context-activated engram neurons following contextual fear conditioning (CFC) and subsequent sleep or sleep deprivation (SD). I find that compared with SD, post-CFC sleep promoted reactivation of more engram cells in the hippocampal dentate gyrus (DG). Interestingly, this reactivation had a subregion-specific pattern, with significantly higher proportion of sleep-dependent reactivation in the inferior blade of DG compared with the superior blade.To characterize learning- and sleep-induced biosynthetic changes in the hippocampus, I used spatial transcriptomics and protein profiling to measure expression changes in the hippocampal DG superior blade, inferior blade, hilus, CA1, and CA3 areas. I found that SD differentially altered the expression of genes in each hippocampal subregion, and hence, differentially impacted various biological pathways in these subregions. I also found that several hours after learning, CFC-induced transcriptomic changes are restricted to the hippocampal DG granule cell layers, and are particularly affecting the synaptic components of neurons. Together, the results identify spatially divergent effects of learning and sleep in hippocampal subregions, which clarify the cellular- and network-level processes altered by sleep disruption.

ISBN: 9798380374408Subjects--Topical Terms:

588700
Neurosciences.
Subjects--Index Terms:

Sleep

Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus.
LDR:03448nmm a2200409 4500 001 2403883
005 20241122094133.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798380374408
035 $a (MiAaPQ)AAI30748423
035 $a (MiAaPQ)umichrackham005114
035 $a AAI30748423
040 $a MiAaPQ $c MiAaPQ
100 1 $a Wang, Lijing. $3 3774166
245 1 0 $a Characterizing Memory Trace Reactivation and Biosynthetic Changes During Post-learning Sleep in the Hippocampus.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 122 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
500 $a Advisor: Aton, Sara Jo.
502 $a Thesis (Ph.D.)--University of Michigan, 2023.
520 $a Over a century of research in both animal models and human subjects shows that sleep may improve, while sleep loss may disrupt, memory task performance. One prevailing hypothesis, active systems consolidation (ASC), posits that during sleep, neurons encoding newly acquired memories are reactivated, and the connections within the network get strengthened, resulting in the formation of a more persistent long-term memory. My thesis tests this hypothesis by quantifying the reactivation of memory traces in the mouse hippocampus during post-learning sleep. I also characterize learning- and sleep-induced biosynthetic changes in different subregions of the hippocampus to clarify the molecular mechanisms associated with this reactivation, in the context of sleep-dependent contextual fear memory consolidation.Using the targeted recombination in active populations (TRAP) mouse line, I quantified reactivation of hippocampal context-activated engram neurons following contextual fear conditioning (CFC) and subsequent sleep or sleep deprivation (SD). I find that compared with SD, post-CFC sleep promoted reactivation of more engram cells in the hippocampal dentate gyrus (DG). Interestingly, this reactivation had a subregion-specific pattern, with significantly higher proportion of sleep-dependent reactivation in the inferior blade of DG compared with the superior blade.To characterize learning- and sleep-induced biosynthetic changes in the hippocampus, I used spatial transcriptomics and protein profiling to measure expression changes in the hippocampal DG superior blade, inferior blade, hilus, CA1, and CA3 areas. I found that SD differentially altered the expression of genes in each hippocampal subregion, and hence, differentially impacted various biological pathways in these subregions. I also found that several hours after learning, CFC-induced transcriptomic changes are restricted to the hippocampal DG granule cell layers, and are particularly affecting the synaptic components of neurons. Together, the results identify spatially divergent effects of learning and sleep in hippocampal subregions, which clarify the cellular- and network-level processes altered by sleep disruption.
590 $a School code: 0127.
650 4 $a Neurosciences. $3 588700
650 4 $a Psychobiology. $3 555678
650 4 $a Molecular biology. $3 517296
650 4 $a Cognitive psychology. $3 523881
653 $a Sleep
653 $a Long-term memory
653 $a Hippocampus
653 $a Active systems consolidation
653 $a Sleep disruption
690 $a 0317
690 $a 0349
690 $a 0633
690 $a 0307
710 2 $a University of Michigan. $b Molecular, Cellular, and Developmental Biology. $3 3347360
773 0 $t Dissertations Abstracts International $g 85-03B.
790 $a 0127
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30748423