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Development and Validation of the Hu...

Lundin, Brady F.

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Development and Validation of the Human Rosette Array for Neural Tube Defect Risk Screening.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development and Validation of the Human Rosette Array for Neural Tube Defect Risk Screening./
Author:	Lundin, Brady F.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2024,
Description:	98 p.
Notes:	Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
Contained By:	Dissertations Abstracts International85-11B.
Subject:	Bioengineering. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31239092
ISBN:	9798382404998

Development and Validation of the Human Rosette Array for Neural Tube Defect Risk Screening.
Lundin, Brady F.

Development and Validation of the Human Rosette Array for Neural Tube Defect Risk Screening. - Ann Arbor : ProQuest Dissertations & Theses, 2024 - 98 p.

Source: Dissertations Abstracts International, Volume: 85-11, Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2024.

Neural tube defects are the second most common congenital malformation and inflict significant morbidity and mortality upon affected patients. The mechanisms by with they form are unclear and traditional methods of studying the nascent central nervous system have significant limitations. Neural organoids have revolutionized how human neurodevelopmental disorders are studied. Yet, their utility for screening complex neural tube defect etiologies and potential prophylactics is limited by a lack of scalable and quantifiable derivation formats. Here, a brief introduction to neural tube formation and an overview of the known causes of neural tube defects is presented. Then a survey of the rapidly developing field of neural organoids is provided. After which, we describe the Rosette Array platform's ability to be used as an off-the-shelf, 96-well plate assay that standardizes incipient forebrain and spinal cord organoid morphogenesis as micropatterned, 3-D, singularly polarized neural rosette tissues. Rosette Arrays are seeded from cryopreserved human pluripotent stem cells, cultured over 6-8 days, and immunostained images can be quantified using artificial intelligence-based software. We demonstrate the platform's suitability for screening genetic and environmental factors known to cause neural tube defect risk. Lastly, the discussion closes by considering the implications of the platform's development and screening results and future research directions regarding understanding and preventing neural tube defect formation.

ISBN: 9798382404998Subjects--Topical Terms:

657580
Bioengineering.
Subjects--Index Terms:

Disease modeling

Development and Validation of the Human Rosette Array for Neural Tube Defect Risk Screening.
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260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2024
300 $a 98 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
500 $a Advisor: Ashton, Randolph S.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2024.
520 $a Neural tube defects are the second most common congenital malformation and inflict significant morbidity and mortality upon affected patients. The mechanisms by with they form are unclear and traditional methods of studying the nascent central nervous system have significant limitations. Neural organoids have revolutionized how human neurodevelopmental disorders are studied. Yet, their utility for screening complex neural tube defect etiologies and potential prophylactics is limited by a lack of scalable and quantifiable derivation formats. Here, a brief introduction to neural tube formation and an overview of the known causes of neural tube defects is presented. Then a survey of the rapidly developing field of neural organoids is provided. After which, we describe the Rosette Array platform's ability to be used as an off-the-shelf, 96-well plate assay that standardizes incipient forebrain and spinal cord organoid morphogenesis as micropatterned, 3-D, singularly polarized neural rosette tissues. Rosette Arrays are seeded from cryopreserved human pluripotent stem cells, cultured over 6-8 days, and immunostained images can be quantified using artificial intelligence-based software. We demonstrate the platform's suitability for screening genetic and environmental factors known to cause neural tube defect risk. Lastly, the discussion closes by considering the implications of the platform's development and screening results and future research directions regarding understanding and preventing neural tube defect formation.
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650 4 $a Neurosciences. $3 588700
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650 4 $a Biomedical engineering. $3 535387
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