東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

CRISPR-Cas Enabled DNA Nanotechnolog...

Kachwala, Mahera J.

Linked to FindBook

Google Book

Amazon

博客來

CRISPR-Cas Enabled DNA Nanotechnology for Advanced Pathogen Detection.

Record Type:	Electronic resources : Monograph/item
Title/Author:	CRISPR-Cas Enabled DNA Nanotechnology for Advanced Pathogen Detection./
Author:	Kachwala, Mahera J.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
Description:	166 p.
Notes:	Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
Contained By:	Dissertations Abstracts International85-06B.
Subject:	Biochemistry. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30818032
ISBN:	9798381170566

CRISPR-Cas Enabled DNA Nanotechnology for Advanced Pathogen Detection.
Kachwala, Mahera J.

CRISPR-Cas Enabled DNA Nanotechnology for Advanced Pathogen Detection. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 166 p.

Source: Dissertations Abstracts International, Volume: 85-06, Section: B.

Thesis (Ph.D.)--State University of New York at Albany, 2023.

The ever-growing list of carcinogens, toxicants, pathogens, and other disease-causing agents necessitates the development of new and improved mechanisms of disease detection. A better understanding of events leading up to the diseased state and early diagnosis is imperative for the timely management and targeted treatment of diseases affecting the biological world. Recent achievements in DNA nanotechnology have led to the prominence of DNA-based technologies for multiplexed biological sensing, high-throughput analysis, and improved diagnostics. Typical methods of disease detection employ complex multi-step procedures and advanced equipment, which significantly reduces their accessibility while elevating their cost. DNA functionalized sensing systems that examine the molecular level changes taking place during disease progression thus serve as good alternatives for diagnostic tests and biomarker detection.{A0}Here, we have developed three distinct methods for pathogen and disease detection. The first method utilizes a cost-efficient gel electrophoresis-based approach capable of detecting as little as 1.5 femtomoles of two conserved viral DNA sequences - tobacco curly shoot virus (TCSV) and hepatitis B virus (HBV). The second method is a three-tiered isothermal diagnostic called 'recombinase amplified CRISPR enhanced chain reaction', abbreviated as 'RACECAR', which enables whole-genome detection and can quantify up to 40 copies of the genome using a fluorescence signal output. Notably, RACECAR can directly detect viral genomes in serum samples without the need for an extraction step. Finally, the third project involves the development of a paper-based sensor for colorimetric and visual detection of Salmonella. This paper diagnostic incorporates principles of synthetic biology by employing toehold switch riboregulators as the signal transducers for cell-free protein translation on paper, along with CRISPR-Cas12a for modular recognition of target nucleic acids.

ISBN: 9798381170566Subjects--Topical Terms:

518028
Biochemistry.
Subjects--Index Terms:

Carcinogens

CRISPR-Cas Enabled DNA Nanotechnology for Advanced Pathogen Detection.
LDR:03192nmm a2200409 4500 001 2401333
005 20241022112138.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798381170566
035 $a (MiAaPQ)AAI30818032
035 $a AAI30818032
035 $a 2401333
040 $a MiAaPQ $c MiAaPQ
100 1 $a Kachwala, Mahera J. $0 (orcid)0000-0001-7157-5563 $3 3771424
245 1 0 $a CRISPR-Cas Enabled DNA Nanotechnology for Advanced Pathogen Detection.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 166 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
500 $a Advisor: Yigit, Mehmet.
502 $a Thesis (Ph.D.)--State University of New York at Albany, 2023.
520 $a The ever-growing list of carcinogens, toxicants, pathogens, and other disease-causing agents necessitates the development of new and improved mechanisms of disease detection. A better understanding of events leading up to the diseased state and early diagnosis is imperative for the timely management and targeted treatment of diseases affecting the biological world. Recent achievements in DNA nanotechnology have led to the prominence of DNA-based technologies for multiplexed biological sensing, high-throughput analysis, and improved diagnostics. Typical methods of disease detection employ complex multi-step procedures and advanced equipment, which significantly reduces their accessibility while elevating their cost. DNA functionalized sensing systems that examine the molecular level changes taking place during disease progression thus serve as good alternatives for diagnostic tests and biomarker detection.{A0}Here, we have developed three distinct methods for pathogen and disease detection. The first method utilizes a cost-efficient gel electrophoresis-based approach capable of detecting as little as 1.5 femtomoles of two conserved viral DNA sequences - tobacco curly shoot virus (TCSV) and hepatitis B virus (HBV). The second method is a three-tiered isothermal diagnostic called 'recombinase amplified CRISPR enhanced chain reaction', abbreviated as 'RACECAR', which enables whole-genome detection and can quantify up to 40 copies of the genome using a fluorescence signal output. Notably, RACECAR can directly detect viral genomes in serum samples without the need for an extraction step. Finally, the third project involves the development of a paper-based sensor for colorimetric and visual detection of Salmonella. This paper diagnostic incorporates principles of synthetic biology by employing toehold switch riboregulators as the signal transducers for cell-free protein translation on paper, along with CRISPR-Cas12a for modular recognition of target nucleic acids.
590 $a School code: 0668.
650 4 $a Biochemistry. $3 518028
650 4 $a Chemistry. $3 516420
650 4 $a Molecular biology. $3 517296
650 4 $a Pathology. $3 643180
653 $a Carcinogens
653 $a Hepatitis B virus
653 $a Riboregulators
653 $a Tobacco curly shoot virus
653 $a Pathogens
690 $a 0487
690 $a 0485
690 $a 0307
690 $a 0571
710 2 $a State University of New York at Albany. $b Chemistry. $3 1064619
773 0 $t Dissertations Abstracts International $g 85-06B.
790 $a 0668
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30818032