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Vascular Cell and Membrane Permeabil...

Younger, Scott.

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Vascular Cell and Membrane Permeability: Biophysical Mechanisms and Experimental Techniques.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Vascular Cell and Membrane Permeability: Biophysical Mechanisms and Experimental Techniques./
Author:	Younger, Scott.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
Description:	139 p.
Notes:	Source: Dissertations Abstracts International, Volume: 85-05, Section: B.
Contained By:	Dissertations Abstracts International85-05B.
Subject:	Biomedical engineering. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30636159
ISBN:	9798380623964

Vascular Cell and Membrane Permeability: Biophysical Mechanisms and Experimental Techniques.
Younger, Scott.

Vascular Cell and Membrane Permeability: Biophysical Mechanisms and Experimental Techniques. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 139 p.

Source: Dissertations Abstracts International, Volume: 85-05, Section: B.

Thesis (Ph.D.)--The University of Arizona, 2023.

Semipermeable cellular barriers play fundamental roles in human physiology and pathobiology. These include the plasma membrane which separates the cellular cytoplasm from the extracellular space and the endothelial cell barrier which separates the internal components of tissue from the circulating blood. The loss of plasma membranes barrier integrity leads to loss of ionic homeostasis, increased permeability through paracellular gaps and to potentially irreversible organ dysfunction. This dissertation describes the biophysical mechanisms and techniques employed toinvestigate mechanisms of permeability regulation involving: A) the amyloid protein, Medin, in pore formation in lipid bilayers which leads to increased ionic permeability, and; B) key cytoskeletal regulator proteins in paracellular gap formation/restoration and modulation of vascular endothelial cell (EC) stiffness including the key cytoskeletal regulator protein, EVL, a member of the ENA-VASP family of proteins. These were tested using electrophysiology, optical microscopy, atomic force microscopy (AFM), and other biophysical techniques. The combination of optical, electrical, and force measurements provided unique insights into the mechanisms underpinning barrier dysfunction and recovery. The tools and techniques created during this project, including polymer-based constructs for the formation of paracellular gaps to mimic tissue injury, a force measurement device to determine lamellipodial protrusion forces based on combining AFM and optical microscopy components together, were designed to be adaptable to study similar barrier-effectors. Thus, the findings described here, as well as the experimental and analysis techniques developed during this work, have the potential to substantially contribute to the understanding of vascular and plasma membrane barrier regulation that influence the severity of multiple inflammatory disorders.

ISBN: 9798380623964Subjects--Topical Terms:

535387
Biomedical engineering.
Subjects--Index Terms:

Actin effector

Vascular Cell and Membrane Permeability: Biophysical Mechanisms and Experimental Techniques.
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300 $a 139 p.
500 $a Source: Dissertations Abstracts International, Volume: 85-05, Section: B.
500 $a Advisor: Garcia, Joe G. N.
502 $a Thesis (Ph.D.)--The University of Arizona, 2023.
520 $a Semipermeable cellular barriers play fundamental roles in human physiology and pathobiology. These include the plasma membrane which separates the cellular cytoplasm from the extracellular space and the endothelial cell barrier which separates the internal components of tissue from the circulating blood. The loss of plasma membranes barrier integrity leads to loss of ionic homeostasis, increased permeability through paracellular gaps and to potentially irreversible organ dysfunction. This dissertation describes the biophysical mechanisms and techniques employed toinvestigate mechanisms of permeability regulation involving: A) the amyloid protein, Medin, in pore formation in lipid bilayers which leads to increased ionic permeability, and; B) key cytoskeletal regulator proteins in paracellular gap formation/restoration and modulation of vascular endothelial cell (EC) stiffness including the key cytoskeletal regulator protein, EVL, a member of the ENA-VASP family of proteins. These were tested using electrophysiology, optical microscopy, atomic force microscopy (AFM), and other biophysical techniques. The combination of optical, electrical, and force measurements provided unique insights into the mechanisms underpinning barrier dysfunction and recovery. The tools and techniques created during this project, including polymer-based constructs for the formation of paracellular gaps to mimic tissue injury, a force measurement device to determine lamellipodial protrusion forces based on combining AFM and optical microscopy components together, were designed to be adaptable to study similar barrier-effectors. Thus, the findings described here, as well as the experimental and analysis techniques developed during this work, have the potential to substantially contribute to the understanding of vascular and plasma membrane barrier regulation that influence the severity of multiple inflammatory disorders.
590 $a School code: 0009.
650 4 $a Biomedical engineering. $3 535387
650 4 $a Physiology. $3 518431
650 4 $a Pathology. $3 643180
650 4 $a Molecular biology. $3 517296
650 4 $a Biophysics. $3 518360
653 $a Actin effector
653 $a Amyloid
653 $a Barrier restoration
653 $a Cytoskeleton
653 $a Electrophysiology
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710 2 $a The University of Arizona. $b Biomedical Engineering. $3 2092025
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791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30636159