東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Lung Epithelial Identity, Plasticity...

Weiner, Aaron I.

FindBook

Google Book

Amazon

博客來

Lung Epithelial Identity, Plasticity, and Fate Choice During Injury Repair and Regeneration.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Lung Epithelial Identity, Plasticity, and Fate Choice During Injury Repair and Regeneration./
作者:	Weiner, Aaron I.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2023,
面頁冊數:	192 p.
附註:	Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
Contained By:	Dissertations Abstracts International84-12B.
標題:	Cellular biology. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30318912
ISBN:	9798379756598

Lung Epithelial Identity, Plasticity, and Fate Choice During Injury Repair and Regeneration.
Weiner, Aaron I.

Lung Epithelial Identity, Plasticity, and Fate Choice During Injury Repair and Regeneration. - Ann Arbor : ProQuest Dissertations & Theses, 2023 - 192 p.

Source: Dissertations Abstracts International, Volume: 84-12, Section: B.

Thesis (Ph.D.)--University of Pennsylvania, 2023.

The lung is unique among mammalian vital organs; while it exhibits low cellular turnover during homeostasis, the lung can regenerate through the widespread proliferation and differentiation of resident progenitor cell populations upon injury. Epithelial insults and respiratory infections such as influenza can disrupt the gas exchange units of the lung, the alveoli, destroying alveolar epithelial type 2 (AT2) and type 1 (AT1) populations. Following such injuries, quiescent lung-resident epithelial progenitors become activated and participate in two distinct reparative pathways: functionally beneficial regeneration via injury-surviving AT2 proliferation and differentiation (which gives rise to more AT2s along with gas-exchanging AT1s), and dysplastic tissue remodeling via intrapulmonary airway-resident basal p63+ progenitors (which generates ectopic airway cell types in the alveolar space that are not thought to perform gas exchange). Utilizing in vivo genetic deletion and lineage tracing, orthotopic progenitor transplantation, in vitro organoid assays, and genome- and epigenome-wide sequencing, this work aims to understand the cellular plasticity inherent to both cell types and elucidate factors that convey their identity in order to promote functional regeneration of the alveolar epithelium and restore the oxygen-exchanging capacity of the damaged lung. We find that AT2 expansion ex vivo for their potential use as an exogenous source of regenerative alveolar progenitors fundamentally alters their identity, causing them to aberrantly form dysplastic tissue when transplanted. However, primary uncultured AT2 transplants retain their lineage fidelity and perform functionally beneficial regeneration as expected, ultimately enhancing lung function. We additionally find that the basal cell transcription factor ΔNp63 is required for intrapulmonary basal progenitors to participate in dysplastic alveolar remodeling following injury and that ΔNp63 restricts the plasticity of intrapulmonary basal progenitors by maintaining their epigenetic landscape. Following loss of ΔNp63, intrapulmonary basal progenitors are capable of either airway or alveolar differentiation depending on their surrounding environment both in vitro and in vivo, in the latter scenario converting into AT2s capable of generating AT1s. These orthogonal approaches elucidate critical regulatory mechanisms of fate choice and identity in these important players in lung repair, highlighting potential therapeutic targets to promote functional alveolar regeneration following severe lung injuries.

ISBN: 9798379756598Subjects--Topical Terms:

3172791
Cellular biology.
Subjects--Index Terms:

Epithelial dysplasia

Lung Epithelial Identity, Plasticity, and Fate Choice During Injury Repair and Regeneration.
LDR:03763nmm a2200385 4500 001 2401049
005 20241015112506.5
006 m o d
007 cr#unu||||||||
008 251215s2023 ||||||||||||||||| ||eng d
020 $a 9798379756598
035 $a (MiAaPQ)AAI30318912
035 $a AAI30318912
035 $a 2401049
040 $a MiAaPQ $c MiAaPQ
100 1 $a Weiner, Aaron I. $3 3771110
245 1 0 $a Lung Epithelial Identity, Plasticity, and Fate Choice During Injury Repair and Regeneration.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2023
300 $a 192 p.
500 $a Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
500 $a Advisor: Vaughan, Andrew E.
502 $a Thesis (Ph.D.)--University of Pennsylvania, 2023.
520 $a The lung is unique among mammalian vital organs; while it exhibits low cellular turnover during homeostasis, the lung can regenerate through the widespread proliferation and differentiation of resident progenitor cell populations upon injury. Epithelial insults and respiratory infections such as influenza can disrupt the gas exchange units of the lung, the alveoli, destroying alveolar epithelial type 2 (AT2) and type 1 (AT1) populations. Following such injuries, quiescent lung-resident epithelial progenitors become activated and participate in two distinct reparative pathways: functionally beneficial regeneration via injury-surviving AT2 proliferation and differentiation (which gives rise to more AT2s along with gas-exchanging AT1s), and dysplastic tissue remodeling via intrapulmonary airway-resident basal p63+ progenitors (which generates ectopic airway cell types in the alveolar space that are not thought to perform gas exchange). Utilizing in vivo genetic deletion and lineage tracing, orthotopic progenitor transplantation, in vitro organoid assays, and genome- and epigenome-wide sequencing, this work aims to understand the cellular plasticity inherent to both cell types and elucidate factors that convey their identity in order to promote functional regeneration of the alveolar epithelium and restore the oxygen-exchanging capacity of the damaged lung. We find that AT2 expansion ex vivo for their potential use as an exogenous source of regenerative alveolar progenitors fundamentally alters their identity, causing them to aberrantly form dysplastic tissue when transplanted. However, primary uncultured AT2 transplants retain their lineage fidelity and perform functionally beneficial regeneration as expected, ultimately enhancing lung function. We additionally find that the basal cell transcription factor ΔNp63 is required for intrapulmonary basal progenitors to participate in dysplastic alveolar remodeling following injury and that ΔNp63 restricts the plasticity of intrapulmonary basal progenitors by maintaining their epigenetic landscape. Following loss of ΔNp63, intrapulmonary basal progenitors are capable of either airway or alveolar differentiation depending on their surrounding environment both in vitro and in vivo, in the latter scenario converting into AT2s capable of generating AT1s. These orthogonal approaches elucidate critical regulatory mechanisms of fate choice and identity in these important players in lung repair, highlighting potential therapeutic targets to promote functional alveolar regeneration following severe lung injuries.
590 $a School code: 0175.
650 4 $a Cellular biology. $3 3172791
650 4 $a Molecular biology. $3 517296
650 4 $a Physiology. $3 518431
653 $a Epithelial dysplasia
653 $a Lung injury
653 $a Lung regeneration
653 $a Lung stem cell biology
690 $a 0379
690 $a 0307
690 $a 0719
710 2 $a University of Pennsylvania. $b Cell and Molecular Biology. $3 2101694
773 0 $t Dissertations Abstracts International $g 84-12B.
790 $a 0175
791 $a Ph.D.
792 $a 2023
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30318912