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Integrated circuit/microfluidic chip...

Hunt, Thomas.

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Integrated circuit/microfluidic chips for dielectric manipulation.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Integrated circuit/microfluidic chips for dielectric manipulation./
Author:	Hunt, Thomas.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2007,
Description:	140 p.
Notes:	Source: Dissertations Abstracts International, Volume: 69-03, Section: B.
Contained By:	Dissertations Abstracts International69-03B.
Subject:	Biomedical research. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3264986
ISBN:	9780549037378

Integrated circuit/microfluidic chips for dielectric manipulation.
Hunt, Thomas.

Integrated circuit/microfluidic chips for dielectric manipulation. - Ann Arbor : ProQuest Dissertations & Theses, 2007 - 140 p.

Source: Dissertations Abstracts International, Volume: 69-03, Section: B.

Thesis (Ph.D.)--Harvard University, 2007.

.

This thesis describes the development of integrated circuit/microfluidic chips to move individual living cells and chemical droplets along programmable paths with dielectrophoresis (DEP). The complexity of cellular and molecular biology demands powerful research techniques. Integrated circuits (ICs) offer high bandwidth, massive parallelism, submicron features, built in programmability and superb control of local electric fields. At the same time, microfluidics provide a suitable environment for both living cells and biochemistry. Our IC/microfluidic chip is an exciting technology capable of simultaneously and independently controlling the location of thousands of objects such as cells and chemical droplets. Dielectrophoresis is the movement of a particle in a non-uniform electric field due to the induced dipole moment of the particle relative to the surrounding medium. By applying an appropriate local electric field, any particle with a dielectric constant different than the surrounding medium can be manipulated with DEP. We initially fabricated an array of microscale post-shaped electrodes to provide an inhomogeneous electric field at the bottom of a microfluidic channel. The voltage on each electrode was independently controlled by a computer to trap and move cells and particles in fluid above the micropost electrodes. We subsequently designed an IC chip consisting of 256 x 128 metal pixels, 11 μm square, with built in control circuits for energizing each pixel to 5 V at frequencies from DC to 1.8 MHz. The chip was built in a commercial foundry and we fabricated a microfluidic channel on its top surface. By shifting the location of energized pixels, electric fields were generated to move yeast and mammalian cells through the microfluidic channel at 30 μm per second. Complex electric field patterns were generated to simultaneously move thousands of individual cells. The chip was capable of translating, splitting, and mixing pL water droplets in oil. In addition to the IC/microfluidic system, we have developed a PDMS microfluidic device that sorts pL drops of water in oil at 1.6 kHz with DEP and a device for separating magnetically tagged bacteria from whole blood at 10k cells per second. We have also built a DEP trap integrated into the tip of a scanning probe for cell and nanoparticle manipulation.

ISBN: 9780549037378Subjects--Topical Terms:

3433833
Biomedical research.
Subjects--Index Terms:

Dielectric manipulation

Integrated circuit/microfluidic chips for dielectric manipulation.
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502 $a Thesis (Ph.D.)--Harvard University, 2007.
506 $a .
520 $a This thesis describes the development of integrated circuit/microfluidic chips to move individual living cells and chemical droplets along programmable paths with dielectrophoresis (DEP). The complexity of cellular and molecular biology demands powerful research techniques. Integrated circuits (ICs) offer high bandwidth, massive parallelism, submicron features, built in programmability and superb control of local electric fields. At the same time, microfluidics provide a suitable environment for both living cells and biochemistry. Our IC/microfluidic chip is an exciting technology capable of simultaneously and independently controlling the location of thousands of objects such as cells and chemical droplets. Dielectrophoresis is the movement of a particle in a non-uniform electric field due to the induced dipole moment of the particle relative to the surrounding medium. By applying an appropriate local electric field, any particle with a dielectric constant different than the surrounding medium can be manipulated with DEP. We initially fabricated an array of microscale post-shaped electrodes to provide an inhomogeneous electric field at the bottom of a microfluidic channel. The voltage on each electrode was independently controlled by a computer to trap and move cells and particles in fluid above the micropost electrodes. We subsequently designed an IC chip consisting of 256 x 128 metal pixels, 11 μm square, with built in control circuits for energizing each pixel to 5 V at frequencies from DC to 1.8 MHz. The chip was built in a commercial foundry and we fabricated a microfluidic channel on its top surface. By shifting the location of energized pixels, electric fields were generated to move yeast and mammalian cells through the microfluidic channel at 30 μm per second. Complex electric field patterns were generated to simultaneously move thousands of individual cells. The chip was capable of translating, splitting, and mixing pL water droplets in oil. In addition to the IC/microfluidic system, we have developed a PDMS microfluidic device that sorts pL drops of water in oil at 1.6 kHz with DEP and a device for separating magnetically tagged bacteria from whole blood at 10k cells per second. We have also built a DEP trap integrated into the tip of a scanning probe for cell and nanoparticle manipulation.
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